Cell No. : Cell Name
RCB0560 : F5-5.fl
update : 2024/01/17
|
| Comment | Friend leukemia. Differentiate to erythroblastoid cells with benzidine etc. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ikawa, Yoji
|
| Originator |
Ikawa, Yoji
|
| Year of deposit |
1991
|
| Original cell |
T-3-cl-2
|
| Animal |
_mouse
< Mammals
|
| Strain name |
DDD
|
| Gender |
Unknown
|
| Tissue |
spleen
|
| Classification |
cancer
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_4177
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
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Free
|
|
Passage method |
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dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
7
|
| User's Publication |
|
12678
Swain A, Kulkeaw K, Tanaka Y, Nakanishi Y, Shirasawa S, Sugiyama D.
DBA Lectin Binds to Highly Proliferative Mouse Erythroleukemia Cells
Anticancer Res
2016
36(7):3625-33
PubMed ID: 27354633
|
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7504
Izumi G, Koga K, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Ogawa K, Inoue S, Fujii T, Osuga Y.
Cyclic stretch augments production of neutrophil chemokines, matrix metalloproteinases, and activin a in human endometrial stromal cells.
Am. J. Reprod. Immunol.
2015
73:501-6
PubMed ID: 25605062
DOI: 10.1111/aji.12359
|
|
6107
Kulkeaw K, Inoue T, Mizuochi C, Horio Y, Ishihama Y, Sugiyama D.
Ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro.
Cell Biol Int
2012
36(2):195-202
PubMed ID: 21988615
DOI: 10.1042/CBI20110169
|
|
7958
Uehara T, Kondo C, Yamate J, Torii M, Maruyama T.
A toxicogenomic approach for identifying biomarkers for myelosuppressive anemia in rats.
Toxicology
2011
282:139-45
PubMed ID: 21296123
DOI: 10.1016/j.tox.2011.01.027
|
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19141
Ogawa K, Funaba M, Tsujimoto M.
A dual role of activin A in regulating immunoglobulin production of B cells
J Leukoc Biol
2008
83(6):1451-8
PubMed ID: 18353928
DOI: 10.1189/jlb.1007710
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6253
Machida H, Ogawa K, Funaba M, Mizutani T, Tsujimoto M.
mRNA expression of type I and type II receptors for activin, transforming growth factor-beta, and bone morphogenetic protein in the murine erythroleukemic cell line, F5-5.fl.
Eur J Endocrinol.
2000
143(5):705-10
PubMed ID: 11078996
DOI: 10.1530/eje.0.1430705
|
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11032
K Kitamura, S i Aota, R Sakamoto, S I Yoshikawa, K Okazaki
Smad7 selectively interferes with different pathways of activin signaling and inhibits erythroid leukemia cell differentiation
Blood
2000
95(11):3371-9
PubMed ID: 10828018
|
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