Back Back
Cell No. : Cell Name
RCB0560 : F5-5.fl  update : 2024/01/17
CommentFriend leukemia. Differentiate to erythroblastoid cells with benzidine etc.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ikawa, Yoji
Originator Ikawa, Yoji
Year of deposit 1991
Original cell T-3-cl-2
Animal _mouse < Mammals
Strain name DDD
Gender Unknown
Tissue spleen
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4177
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 7

To topTop
4768  Matsugi T, Amanuma H, Ikawa Y.  Presence of a novel spleen focus-forming virus-specific glycoprotein (gp51) in a Friend leukemia cell line and its decrease during erythrodifferentiation.  Gan  1983  74(4):509-16  PubMed ID: 6628903  
1372  Ikawa Y, Aida M, Inoue Y  Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.  Gann  1976  67:767-70  PubMed ID: 1071084  

To topTop
User's Publication
12678  Swain A, Kulkeaw K, Tanaka Y, Nakanishi Y, Shirasawa S, Sugiyama D.  DBA Lectin Binds to Highly Proliferative Mouse Erythroleukemia Cells  Anticancer Res  2016  36(7):3625-33  PubMed ID: 27354633  
7504  Izumi G, Koga K, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Ogawa K, Inoue S, Fujii T, Osuga Y.  Cyclic stretch augments production of neutrophil chemokines, matrix metalloproteinases, and activin a in human endometrial stromal cells.  Am. J. Reprod. Immunol.  2015  73:501-6  PubMed ID: 25605062   DOI: 10.1111/aji.12359
6107  Kulkeaw K, Inoue T, Mizuochi C, Horio Y, Ishihama Y, Sugiyama D.  Ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro.  Cell Biol Int  2012  36(2):195-202  PubMed ID: 21988615   DOI: 10.1042/CBI20110169
7958  Uehara T, Kondo C, Yamate J, Torii M, Maruyama T.  A toxicogenomic approach for identifying biomarkers for myelosuppressive anemia in rats.  Toxicology  2011  282:139-45  PubMed ID: 21296123   DOI: 10.1016/j.tox.2011.01.027
19141  Ogawa K, Funaba M, Tsujimoto M.  A dual role of activin A in regulating immunoglobulin production of B cells  J Leukoc Biol  2008  83(6):1451-8  PubMed ID: 18353928   DOI: 10.1189/jlb.1007710
6253  Machida H, Ogawa K, Funaba M, Mizutani T, Tsujimoto M.  mRNA expression of type I and type II receptors for activin, transforming growth factor-beta, and bone morphogenetic protein in the murine erythroleukemic cell line, F5-5.fl.  Eur J Endocrinol.  2000  143(5):705-10  PubMed ID: 11078996   DOI: 10.1530/eje.0.1430705
11032  K Kitamura, S i Aota, R Sakamoto, S I Yoshikawa, K Okazaki  Smad7 selectively interferes with different pathways of activin signaling and inhibits erythroid leukemia cell differentiation  Blood  2000  95(11):3371-9  PubMed ID: 10828018  

Back Back Return Top Page