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Cell No. : Cell Name
RCB0559 : K-1.fl  update : 2022/03/17
CommentSFFV producing Friend leukemia cell line. Non-differentiating.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ikawa, Yoji
Originator Ikawa, Yoji
Year of deposit 1991
Animal _mouse < Mammals
Strain name DDD
Gender Unknown
Tissue spleen
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_F499
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 8
User's Publication 1

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4782  Aisaki K, Kanno H, Oyaizu N, Hara Y, Miwa S, Ikawa Y.  Apoptotic changes precede mitochondrial dysfunction in red cell-type pyruvate kinase mutant mouse erythroleukemia cell lines.  Jpn J Cancer Res  1999  90(2):171-9  PubMed ID: 10189887   DOI: 10.1111/j.1349-7006.1999.tb00730.x
4781  Xu X, Yamamura Y, Tsukada T, Yoshida MA, Senda H, Nagayoshi M, Ikeuchi T, Ikawa Y.  A mouse erythroleukemia cell line possessing friend spleen focus-forming virus gp55 transgene and temperature-sensitive mutant p53 gene.  Jpn J Cancer Res  1995  86(3):284-91  PubMed ID: 7744699   DOI: 10.1111/j.1349-7006.1995.tb03052.x
4780  Amanuma H, Watanabe N, Nishi M, Ikawa Y.  Requirement of the single base insertion at the 3' end of the env-related gene of Friend spleen focus-forming virus for pathogenic activity and its effect on localization of the glycoprotein product (gp55).  J Virol  1989  63(11):4824-33  PubMed ID: 2552155   DOI: 10.1128/JVI.63.11.4824-4833.1989
3684  Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.  Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.  Biochim Biophys Acta  1988  943(2):326-30  PubMed ID: 2840960   DOI: 10.1016/0005-2736(88)90564-0
3685  Ikawa Y, Obata M, Sagata N, Amanuma H.  Modified env gene in Friend spleen focus forming virus--structure, origin, and its role in leukemogenesis  Gan To Kagaku Ryoho  1985  12(3 Pt 2):622-32  PubMed ID: 3857023  
4778  Obata M, Amanuma H, Harada Y, Sagata N, Ikawa Y.  env-Related leukemogenic genes (gp55 genes) of two closely related polycythemic strains of Friend spleen focus-forming virus possess different recombination points with an endogenous mink cell focus-forming virus env gene.  Virology  1984  136(2):435-8  PubMed ID: 6464355   DOI: 10.1016/0042-6822(84)90179-x
4768  Matsugi T, Amanuma H, Ikawa Y.  Presence of a novel spleen focus-forming virus-specific glycoprotein (gp51) in a Friend leukemia cell line and its decrease during erythrodifferentiation.  Gan  1983  74(4):509-16  PubMed ID: 6628903  
1372  Ikawa Y, Aida M, Inoue Y  Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.  Gann  1976  67:767-70  PubMed ID: 1071084  

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User's Publication
6346  Oka M, Mura S, Yamada K, Sangel P, Hirata S, Maehara K, Kawakami K, Tachibana T, Ohkawa Y, Kimura H, Yoneda Y.  Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes.  Elife  2016  5:e09540  PubMed ID: 26740045   DOI: 10.7554/eLife.09540

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