Cell No. : Cell Name
RCB0559 : K-1.fl
update : 2024/01/17
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Comment | SFFV producing Friend leukemia cell line. Non-differentiating. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ikawa, Yoji
|
Originator |
Ikawa, Yoji
|
Year of deposit |
1991
|
Animal |
_mouse
< Mammals
|
Strain name |
DDD
|
Gender |
Unknown
|
Tissue |
spleen
|
Classification |
cancer
|
Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
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|
Lifespan |
infinite
|
Morphology |
lymphocyte-like
|
Cellosaurus(Expasy) |
CVCL_F499
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
|
Culture medium |
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RPMI1640 + 10% FBS
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Antibiotics |
|
Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
|
5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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SSLP(mouse) |
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OK
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Isozyme |
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LD, NP
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Reference information |
Reference |
8
|
User's Publication |
1
|
Reference |
4782
Aisaki K, Kanno H, Oyaizu N, Hara Y, Miwa S, Ikawa Y.
Apoptotic changes precede mitochondrial dysfunction in red cell-type pyruvate kinase mutant mouse erythroleukemia cell lines.
Jpn J Cancer Res
1999
90(2):171-9
PubMed ID: 10189887
DOI: 10.1111/j.1349-7006.1999.tb00730.x
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4781
Xu X, Yamamura Y, Tsukada T, Yoshida MA, Senda H, Nagayoshi M, Ikeuchi T, Ikawa Y.
A mouse erythroleukemia cell line possessing friend spleen focus-forming virus gp55 transgene and temperature-sensitive mutant p53 gene.
Jpn J Cancer Res
1995
86(3):284-91
PubMed ID: 7744699
DOI: 10.1111/j.1349-7006.1995.tb03052.x
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4780
Amanuma H, Watanabe N, Nishi M, Ikawa Y.
Requirement of the single base insertion at the 3' end of the env-related gene of Friend spleen focus-forming virus for pathogenic activity and its effect on localization of the glycoprotein product (gp55).
J Virol
1989
63(11):4824-33
PubMed ID: 2552155
DOI: 10.1128/JVI.63.11.4824-4833.1989
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3684
Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.
Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.
Biochim Biophys Acta
1988
943(2):326-30
PubMed ID: 2840960
DOI: 10.1016/0005-2736(88)90564-0
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3685
Ikawa Y, Obata M, Sagata N, Amanuma H.
Modified env gene in Friend spleen focus forming virus--structure, origin, and its role in leukemogenesis
Gan To Kagaku Ryoho
1985
12(3 Pt 2):622-32
PubMed ID: 3857023
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4778
Obata M, Amanuma H, Harada Y, Sagata N, Ikawa Y.
env-Related leukemogenic genes (gp55 genes) of two closely related polycythemic strains of Friend spleen focus-forming virus possess different recombination points with an endogenous mink cell focus-forming virus env gene.
Virology
1984
136(2):435-8
PubMed ID: 6464355
DOI: 10.1016/0042-6822(84)90179-x
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4768
Matsugi T, Amanuma H, Ikawa Y.
Presence of a novel spleen focus-forming virus-specific glycoprotein (gp51) in a Friend leukemia cell line and its decrease during erythrodifferentiation.
Gan
1983
74(4):509-16
PubMed ID: 6628903
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1372
Ikawa Y, Aida M, Inoue Y
Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.
Gann
1976
67:767-70
PubMed ID: 1071084
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