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細胞番号 : 細胞名
RCB0558 : LLC  update : 2021/01/08
細胞特性(Comment:英)Highly metastatic and drug-resistant mouse tumor. Application consideration
細胞特性(日)転移能が非常に高いマウス肺癌。各種の制癌剤に抵抗性があり in vivoで継代していたものを培養化した。Lewis lung carcinomaが本来の名称である。培養開始にあたっての注意
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Sugimoto, Yoshikazu
樹立者 Sugimoto, Y. (in vitro)
寄託日 1990
別名 Lewis lung carcinoma
動物種 _mouse < Mammals
採取組織 lung
転移 Yes
細胞分類 cancer
細胞寿命 infinite
細胞形態 other
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 4-6 split
継代・培地交換頻度 Subculture : 1-2 times/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 39件
利用者成果(日) 1件

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Reference(英)
1371  Bertram, J S, Janik P  Establishment of a cloned line of Lewis Lung Carcinoma cells adapted to cell culture.  Cancer Lett  1980  11:63-73  PubMed ID: 7226139
4767  Janik P, Assaf A, Bertram JS.  Inhibition of growth of primary and metastatic Lewis lung carcinoma cells by the phosphodiesterase inhibitor isobutylmethylxanthine.  Cancer Res  1980  40(6):1950-4  PubMed ID: 6154533

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Reference(日)

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利用者成果(英)
20055  Watanabe R, Esaki T, Ohashi R, Kuroda M, Kawashima H, Komura H, Natsume-Kitatani Y, Mizuguchi K.  Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration.  J Med Chem  2021    PubMed ID: 33619967
13056  Yoshizawa K, Tabuchi M, Ukai S, Suzuki H, Kawano Y, Takasawa R.  The Putative Glyoxalase 1 Inhibitor Piceatannol Exhibits Both Anxiolytic-like and Antitumor Effects in Mice.  Anticancer Res  2020    PubMed ID: 32487622
4047  Naito H, Iba T, Wakabayashi T, Tai-Nagara I, Suehiro JI, Jia W, Eino D, Sakimoto S, Muramatsu F, Kidoya H, Sakurai H, Satoh T, Akira S, Kubota Y, Takakura N.  TAK1 Prevents Endothelial Apoptosis and Maintains Vascular Integrity  Dev Cell  2019  48(2):151-166  PubMed ID: 30639056
11311  Umakoshi M, Takahashi S, Itoh G, Kuriyama S, Sasaki Y, Yanagihara K, Yashiro M, Maeda D, Goto A, Tanaka M.  Macrophage-mediated transfer of cancer-derived components to stromal cells contributes to establishment of a pro-tumor microenvironment.  Oncogene  2019    PubMed ID: 30459356
11310  Wang Y, Zenkoh J, Gerelchuluun A, Sun L, Cai S, Li X, Tsuboi K.  Administration of Dendritic Cells and Anti-PD-1 Antibody Converts X-ray Irradiated Tumors Into Effective In situ Vaccines.  Int. J. Radiat. Oncol. Biol. Phys.  2019    PubMed ID: 30458232
12503  Yamasuge W, Yamamoto Y, Fujigaki H, Hoshi M, Nakamoto K, Kunisawa K, Mouri A, Nabeshima T, Saito K.  Indoleamine 2,3-dioxygenase 2 depletion suppresses tumor growth in a mouse model of Lewis lung carcinoma  Cancer Sci  2019  110(10):3061-3067  PubMed ID: 31444833
10609  Omori K, Morikawa T, Kunita A, Nakamura T, Aritake K, Urade Y, Fukayama M, Murata T.  Lipocalin-type prostaglandin D synthase-derived PGD2 attenuates malignant properties of tumor endothelial cells.  J. Pathol.  2018  244:84-96  PubMed ID: 29124765
10811  Yamakawa D, Jia W, Kidoya H, Hosojima S, Torigata M, Zhang L, Takakura N.  Visualization of Proliferative Vascular Endothelial Cells in Tumors in Vivo by Imaging Their Partner of Sld5-1 Promoter Activity.  Am. J. Pathol.  2018  188:1300-1314  PubMed ID: 29650228
10663  Okuma A, Hanyu A, Watanabe S, Hara E.  p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis.  Nat Commun  2017  8:2050  PubMed ID: 29234059
6349  Ponzio G, Rezzonico R, Bourget I, Allan R, Nottet N, Popa A, Magnone V, Rios G, Mari B, Barbry P.  A new long noncoding RNA (lncRNA) is induced in cutaneous squamous cell carcinoma and down-regulates several anticancer and cell differentiation genes in mouse.  J Biol Chem.   2017  292(30):12483-12495  PubMed ID: 28596382
7088  Mukai H, Muramatsu A, Mashud R, Kubouchi K, Tsujimoto S, Hongu T, Kanaho Y, Tsubaki M, Nishida S, Shioi G, Danno S, Mehruba M, Satoh R, Sugiura R.  PKN3 is the major regulator of angiogenesis and tumor metastasis in mice.  Sci Rep  2016  6:18979  PubMed ID: 26742562
9613  Wang X, Li X, Yoshiyuki K, Watanabe Y, Sogo Y, Ohno T, Tsuji NM, Ito A.  Comprehensive Mechanism Analysis of Mesoporous-Silica-Nanoparticle-Induced Cancer Immunotherapy.  Adv Healthc Mater  2016  5:1169-76  PubMed ID: 26987867
9643  Naito H, Wakabayashi T, Kidoya H, Muramatsu F, Takara K, Eino D, Yamane K, Iba T, Takakura N.  Endothelial Side Population Cells Contribute to Tumor Angiogenesis and Antiangiogenic Drug Resistance.  Cancer Res.  2016  76:3200-10  PubMed ID: 27197162
9904  Hanaoka Y, Yamaguchi Y, Yamamoto H, Ishii M, Nagase T, Kurihara H, Akishita M, Ouchi Y.  In Vitro and In Vivo Anticancer Activity of Human β-Defensin-3 and Its Mouse Homolog.  Anticancer Res.  2016  36:5999-6004  PubMed ID: 27793926
5392  Tsutsumi K, Kaname T1, Shiraishi H1, Kawashiri T1, Egashira N.  Polaprezinc reduces paclitaxel-induced peripheral neuropathy in rats without affecting anti-tumor activity.  J Pharmacol Sci  2016  131(2):146-9  PubMed ID: 27262900
6338  Yu R, Zheng Y, Lucas MB, Tong YG.  Elusive liver factor that causes pancreatic α cell hyperplasia: A review of literature.  World J Gastrointest Pathophysiol  2015  6(4):131-9  PubMed ID: 26600971
13661  Hasegawa H, Yamashita K, Otubo D, Fujii S, Kamigaki T, Kuroda D, Kakeji Y.  Allogeneic DCG promote lung NK cell activation and antitumor effect after invariant NKT cell activation  Anticancer Res  2014  34(7):3411-7.  PubMed ID: 24982348
5377  Mizumoto S, Takahashi J, Sugahara K.  Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.  J Biol Chem  2012  287(23):18985-94  PubMed ID: 22493510
7872  Hara K, Kasahara E, Takahashi N, Konishi M, Inoue J, Jikumaru M, Kubo S, Okamura H, Sato E, Inoue M.  Mitochondria determine the efficacy of anticancer agents that interact with DNA but not the cytoskeleton.  J. Pharmacol. Exp. Ther.  2011  337:838-45  PubMed ID: 21421738
8218  Yin X, Yan X, Yang Q, Cao H, Liang H.  Antitumor mechanism of recombinant murine interleukin-12 vaccine.  Cancer Biother. Radiopharm.  2010  25:263-8  PubMed ID: 20578831
8326  Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata R.  Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. [corrected]  Transgenic Res.  2010  19:1109-17  PubMed ID: 20333465
8438  Yin XL, Yan X, Wen M, Peng ZP, Li SL.  Synergistic antitumor effects of 131I-LC-1 IgM and IL-12 vaccine on Lewis lung carcinoma.  Int. Immunopharmacol.  2010  10:284-9  PubMed ID: 19951748
5373  Kawashiri T, Egashira N, Itoh Y, Shimazoe T, Ikegami Y, Yano T, Yoshimura M, Oishi R.  Neurotropin reverses paclitaxel-induced neuropathy without affecting anti-tumour efficacy.  Eur J Cancer  2009  45(1):154-63  PubMed ID: 19027286
8763  Jung YK, Jin JS, Jeong JH, Kim HN, Park NR, Choi JY.  DICAM, a novel dual immunoglobulin domain containing cell adhesion molecule interacts with alphavbeta3 integrin.  J. Cell. Physiol.  2008  216:603-14  PubMed ID: 18366072
8812  Yamamura H, Hirano N, Koyama H, Nishizawa Y, Takahashi K.  Loss of smooth muscle calponin results in impaired blood vessel maturation in the tumor-host microenvironment.  Cancer Sci.  2007  98:757-63  PubMed ID: 17391313
3567  Yoshikawa N, Nakamura K, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M.  Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin, an adenosine deaminase inhibitor.  In Vivo  2007  21(2):291-5  PubMed ID: 17436579
6293  Imai N, Hashimoto T, Kihara M, Yoshida S, Kawana I, Yazawa T, Kitamura H, Umemura S.  Roles for host and tumor angiotensin II type 1 receptor in tumor growth and tumor-associated angiogenesis.  Lab Invest  2007  87(2):189-98  PubMed ID: 17318197
3290  Nakamura, Kazuki, Yoshikawa, Noriko, Yamaguchi, Yu, Kagota, Satomi, Shinozuka, Kazumasa, Kunitomo, Masaru  Antitumor effect of cordycepin (3'-deoxyadenosine) on mouse melanoma and lung carcinoma cells involves adenosine A3 receptor stimulation.  Anticancer Res  2006  26:43-7  PubMed ID: 16475677
13654  Kushida S, Ohmae H, Kamma H, Totsuka R, Matsumura M, Takeuchi A, Saiki I, Yanagawa T, Onizawa K, Ishii T, Ohn T.  Artificial cytokine storm combined with hyperthermia induces significant anti-tumor effect in mice inoculated with lewis lung carcinoma and B16 melanoma cells  Int J Hyperthermia  2006  22(8):699-712  PubMed ID: 17390999
3336  Kuang M, Liu, S Q, Saijo K, Uchimura E, Huang L, Leong, K W, Lu, M D, Huang, J F, Ohno T  Microwave tumour coagulation plus in situ treatment with cytokine-microparticles: induction of potent anti-residual tumour immunity.  Int J Hyperthermia  2005  21:247-57  PubMed ID: 16019851
9035  Li-Quan Wang, Byung-Sun Min, Yan Li, Norio Nakamura, Guo-Wei Qin, Can-Jun Li, Masao Hattori  Annonaceous Acetogenins From the Leaves of Annona Montana  Bioorg Med Chem  2002  10 (3), 561-5  PubMed ID: 11814843
2636  Fujisawa H  Inhibitory role of neutrophils on influenza virus multiplication in the lungs of mice.  Microbiol Immunol  2001  45:679-88  PubMed ID: 11762750
5370  Hamada K, Mikasa K, Yunou Y, Kurioka T, Majima T, Narita N, Kita E.  Adjuvant effect of clarithromycin on chemotherapy for murine lung cancer.  Chemotherapy  2000  46(1):49-61  PubMed ID: 10601798
6266  Matsui H, Kimura A, Yamashiki N, Moriyama A, Kaya M, Yoshida I, Takagi N, Takahashi T.  Molecular and biochemical characterization of a serine proteinase predominantly expressed in the medulla oblongata and cerebellar white matter of mouse brain.  J Biol Chem  2000  275(15):11050-7  PubMed ID: 10753908
2355  Nakamura K, Yamaguchi Y, Kagota S, Kwon, Y M, Shinozuka K, Kunitomo M  Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis of Lewis lung carcinoma and B16 melanoma cells in syngeneic mice.  Jpn J Pharmacol  1999  79:335-41  PubMed ID: 10230862
2270  Sumimoto H, Tani K, Nakazaki Y, Tanabe T, Hibino H, Wu, M S, Izawa K, Hamada H, Asano S  Superiority of interleukin-12 taransduced murine lung cancer cells to GM-CSF or B7-1 (CD80) transfectants for therapeutic antitumor immunity in syngeneic immunocompetent mice  Cancer Gene Therapy  1998  5:29-37  PubMed ID: 9476964
3952  Akatsu T, Ono K, Katayama Y, Tamura T, Nishikawa M, Kugai N, Yamamoto M, Nagata N.  The mouse mammary tumor cell line, MMT060562, produces prostaglandin E2 and leukemia inhibitory factor and supports osteoclast formation in vitro via a stromal cell-dependent pathway.  J Bone Miner Res  1998  13(3):400-8  PubMed ID: 9525340
6261  Matsui H, Ohnishi J, Takahashi T.  Proteolytic activation of tissue-type plasminogen activator by the culture media of mouse cancer cells.  Zoolog Sci  1998  15(4):499-505  PubMed ID: 18462028
2199  Sumimoto H, Tani K, Nakazaki Y, Tanabe T, Hibino H, Hamada H, Azuma M, Asano S  GM-CSF and B7-1 (CD80) co-stimulatory signals co-operate in the induction of effective anti-tumor immunity in syngeneic mice.  Int J Cancer  1997  73:556-61  PubMed ID: 9389572

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利用者成果(日)
10980  前花 祥太郎 他  マウス肺がんに対する可溶性VEGFR-3による細胞治療の試み  北里医学  2017  47: 11-19  



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