Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0557 : B16 melanoma 4A5  update : 2021/02/26
CommentMelanin pigment producing mouse melanoma. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugimoto, Yoshinori
Year of deposit 1990
Original cell B16 melanoma
Animal _mouse < Mammals
Strain name C57BL/6
Tissue skin
Disease name melanoma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 40


To topTop
Reference
587  Silagi S  Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance.  J Cell Biol  1969  43:263-74  PubMed ID: 4981070
3488  HU F, LESNEY PF.  THE ISOLATION AND CYTOLOGY OF TWO PIGMENT CELL STRAINS FROM B-16 MOUSE MELANOMAS  Cancer Res  1964  24:1634-43.  PubMed ID: 14234007
547  Riley,V.  Enzymatic determination of transmissible replicating factors associated with mouse tumors.  Ann N Y Acad Sci  1963  100:762-790  PubMed ID: 13973915

To topTop
User's Publication
10006  Tomohiro Itoh, Kanako Fukatani, Ayaka Nakashima, Kengo Suzuki  MicroRNA-141-3p and microRNA-200a-3p Regulate α-Melanocyte Stimulating Hormone-Stimulated Melanogenesis by Directly Targeting Microphthalmia-Associated Transcription Factor  Sci Rep  2020  10(1):2149  PubMed ID: 32034251
12162  Kazuhiro Kagotani, Hiroko Nakayama, Liqing Zang, Yuki Fujimoto, Akihito Hayashi, Ryoji Sono, Norihiro Nishimura, and Yasuhito Shimada  Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide  Molecules  2020    PubMed ID: 32244349
12172  Kosuke Nishi, Mizuki Mori, Daisuke Nakayama, Junna Sato, In-Hae Kim, Minju Kim, Songmun Kim & Takuya Sugahara  Anti-melanogenic activity of methanolic extract from leaves of Sorbaria sorbifolia var. stellipila Max. on α-MSH-stimulated B16 melanoma 4A5 cells  Biomedical Dermatology  2020   
11365  Kawasaki T, Sato A, Tominaga Y, Suzuki Y, Oyama T, Tadokoro M, Tsukiyama K, Nokihara K, Zen H.  Photo-Modification of Melanin by a Mid-infrared Free-electron Laser.  Photochem. Photobiol.  2019    PubMed ID: 30613988
11374  Wang W, Nakashima S, Nakamura S, Oda Y, Matsuda H.  Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42-Rac1-RhoA signaling protein levels.  J Nat Med  2019    PubMed ID: 30627935
11473  Wang W, Zhang Y, Nakashima S, Nakamura S, Wang T, Yoshikawa M, Matsuda H.  Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds.  J Nat Med  2019    PubMed ID: 30847755
5791  Takuya Seko, Shintaro Imamura, Kenji Ishihara, Yumiko Yamashita, Michiaki Yamashita  Selenoneine suppresses melanin synthesis by inhibiting tyrosinase in murine B16 melanoma cells and 3D-cultured human melanocytes  Fisheries Science   2019  1–9 
10603  Taira N, Katsuyama Y, Yoshioka M, Okano Y, Masaki H.  3-O-Glyceryl-2-O-hexyl ascorbate suppresses melanogenesis by interfering with intracellular melanosome transport and suppressing tyrosinase protein synthesis.  J Cosmet Dermatol  2018  17(6):1209-1215  PubMed ID: 29115012
10830  Katsuyama Y, Taira N, Yoshioka M, Okano Y, Masaki H.  3-O-Glyceryl-2-O-hexyl Ascorbate Suppresses Melanogenesis through Activation of the Autophagy System.  Biol. Pharm. Bull.  2018  41:824-827  PubMed ID: 29709921
11067    Compositions and melanogenesis-inhibitory activities of the extracts of defatted shea (Vitellaria paradoxa) kernels from seven African countries  Journal of Food Composition and Analysis  2018  70, 89-97 
10675  Manse Y, Ninomiya K, Nishi R, Hashimoto Y, Chaipech S, Muraoka O, Morikawa T.  Labdane-Type Diterpenes, Galangalditerpenes A-C, with Melanogenesis Inhibitory Activity from the Fruit of Alpinia galanga.  Molecules  2017  22:  PubMed ID: 29261124
7101  Ninomiya K, Matsumoto T, Chaipech S, Miyake S, Katsuyama Y, Tsuboyama A, Pongpiriyadacha Y, Hayakawa T, Muraoka O, Morikawa T.  Simultaneous quantitative analysis of 12 methoxyflavones with melanogenesis inhibitory activity from the rhizomes of Kaempferia parviflora.  J Nat Med  2016  70:179-89  PubMed ID: 26711832
12210  Amal Sallam, Amira Mira, Ahmed Ashour, Kuniyoshi Shimizu  Acetylcholine esterase inhibitors and melanin synthesis inhibitors from Salvia officinalis  Phytomedicine  2016  23(10):1005-11  PubMed ID: 27444345
13684  Horiba H, Nakagawa T, Zhu Q, Ashour A, Watanabe A, Shimizu K.  Biological Activities of Extracts from Different Parts of Cryptomeria japonica  Nat Prod Commun  2016  11(9):1337-1342  PubMed ID: 30807038
7182  Inada M, Takita M, Yokoyama S, Watanabe K, Tominari T, Matsumoto C, Hirata M, Maru Y, Maruyama T, Sugimoto Y, Narumiya S, Uematsu S, Akira S, Murphy G, Nagase H, Miyaura C.  Direct Melanoma Cell Contact Induces Stromal Cell Autocrine Prostaglandin E2-EP4 Receptor Signaling That Drives Tumor Growth, Angiogenesis, and Metastasis.  J. Biol. Chem.  2015  290:29781-93  PubMed ID: 26475855
7574  Yuji SHIBATA, Katsuhisa YAMADA, Chiaki IMADA, Takeshi KOBAYASHI, Takeshi TERAHARA  Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan.  Deep Ocean Water Research  2015  15:117-124 
5330  Nakamura S, Nakashima S, Oda Y, Yokota N, Fujimoto K, Matsumoto T, Ohta T, Ogawa K, Maeda S, Nishida S, Matsuda H, Yoshikawa M.  Alkaloids from Sri Lankan curry-leaf (Murraya koenigii) display melanogenesis inhibitory activity: structures of karapinchamines A and B.  Bioorg Med Chem  2013  21(5):1043-9  PubMed ID: 23376010
6320  Shono M, Yoshioka R, Chatani Y, Hirai Y.  Ectopic expression of syntaxin3 affects behaviors of B16 melanoma by controlling actin dynamics.  Cell Struct Funct  2013  38(1):97-107  PubMed ID: 23546178
5627  Nakamura S, Moriura T, Park S, Fujimoto K, Matsumoto T, Ohta T, Matsuda H, Yoshikawa M.  Melanogenesis inhibitory and fibroblast proliferation accelerating effects of noroleanane- and oleanane-type triterpene oligoglycosides from the flower buds of Camellia japonica.  J Nat Prod  2012  75(8):1425-30  PubMed ID: 22834923
7618  Yoshioka S, Terashita T, Yoshizumi H, Shirasaka N.  Inhibitory effects of whisky polyphenols on melanogenesis in mouse B16 melanoma cells.  Biosci. Biotechnol. Biochem.  2011  75:2278-82  PubMed ID: 22146709
8108  Fujita S, Ninomiya M, Efdi M, Ohguchi K, Nozawa Y, Koketsu M.  Isolation of chemical constituents from Enicosanthum cupulare (King) Airy-Shaw.  Nat. Prod. Res.  2010  24:1630-6  PubMed ID: 20954090
8400  Ohguchi K, Nakajima C, Oyama M, Iinuma M, Itoh T, Akao Y, Nozawa Y, Ito M.  Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki 'Fuyu') on melanin biosynthesis.  Biol. Pharm. Bull.  2010  33:122-4  PubMed ID: 20045948
8513  Matsuyama K, Villareal MO, El Omri A, Han J, Kchouk ME, Isoda H.  Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells.  J Nat Med  2009  63:468-72  PubMed ID: 19685105
8661  Ohguchi K, Ito M, Yokoyama K, Iinuma M, Itoh T, Nozawa Y, Akao Y.  Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells.  Biol. Pharm. Bull.  2009  32:308-10  PubMed ID: 19182396
8715  Kobori M, Ohnishi-Kameyama M, Akimoto Y, Yukizaki C, Yoshida M.  Alpha-eleostearic acid and its dihydroxy derivative are major apoptosis-inducing components of bitter gourd.  J. Agric. Food Chem.  2008  56:10515-20  PubMed ID: 18959405
6287  Roy MK, Kobori M, Takenaka M, Nakahara K, Shinmoto H, Isobe S, Tsushida T.  Antiproliferative effect on human cancer cell lines after treatment with nimbolide extracted from an edible part of the neem tree (Azadirachta indica).  Phytother Res  2007  21(3):245-50  PubMed ID: 17163581
3313  Shoji, Toshihiko, Masumoto, Saeko, Moriichi, Nina, Kobori, Masuko, Kanda, Tomomasa, Shinmoto, Hiroshi, Tsushida, Tojiro  Procyanidin trimers to pentamers fractionated from apple inhibit melanogenesis in B16 mouse melanoma cells.  J Agric Food Chem  2005  53:6105-11  PubMed ID: 16029003
2846  Fukui M, Nakano-Hashimoto T, Okano K, Maruta Y, Suehiro Y, Hamanaka Y, Yamashita H, Imai K, Kawano MM, Hinoda Y  Therapeutic effect of dendritic cells loaded with a fusion mRNA encoding tyrosinase-related protein 2 and enhanced green fluorescence protein on B16 melanoma.  Tumour Biol  2004  25:252-7  PubMed ID: 15627888
3221  Ohguchi, Kenji, Banno, Yoshiko, Akao, Yukihiro, Nozawa, Yoshinori  Involvement of phospholipase D1 in melanogenesis of mouse B16 melanoma cells.  J Biol Chem  2004  279:3408-12  PubMed ID: 14615486
13484  Ohara M, Kobayashi M, Fujiwara H, Kitajima S, Mitsuoka C, Watanabe H.  Blue light inhibits melanin synthesis in B16 melanoma 4A5 cells and skin pigmentation induced by ultraviolet B in guinea-pigs  Photodermatol Photoimmunol Photomed  2004  20(2):86-92  PubMed ID: 15030593
13675  Kuniyoshi Shimizu, Xinglian Geng, Mika Hashiguchi, Hiroto Suhara, Syoko Fukunaga, Seiji Yasutake, Ryuichiro Kondo, Mototake Tsutsui, Ikuo Sato  Indole-3-carbaldehyde: a tyrosinase inhibitor from fungus YL185  Journal of Wood Science  2003  49:349-354 
6185  Matsui H, Takahashi T.  Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3.  Endocrinology  2001  142(11):4918-29  PubMed ID: 11606460
5066  Iwashita K, Kobori M, Yamaki K, Tsushida T.  Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells.  Biosci Biotechnol Biochem  2000  64(9):1813-20  PubMed ID: 11055382
2368  Imaizumi K, Kawabe T, Ichiyama S, Kikutani H, Yagita H, Shimokata K, Hasegawa Y  Enhancement of tumoricidal activity of alveolar macrophages via CD40-CD40 ligand interaction  Am J Physiol  1999  277(1 Pt 1):L49-57  PubMed ID: 10409230
5065  Kobori M, Iwashita K, Shinmoto H, Tsushida T.  Phloretin-induced apoptosis in B16 melanoma 4A5 cells and HL60 human leukemia cells.  Biosci Biotechnol Biochem  1999  63(4):719-25  PubMed ID: 10361685
3525  Zhao X1, Wakamatsu Y, Shibahara M, Nomura N, Geltinger C, Nakahara T, Murata T, Yokoyama KK.  Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture.  Cancer Res  1999  59(2):482-6  PubMed ID: 9927066
5064  Kobori M, Shinmoto H, Tsushida T, Shinohara K.  Phloretin-induced apoptosis in B16 melanoma 4A5 cells by inhibition of glucose transmembrane transport.  Cancer Lett  1997  119(2):207-12  PubMed ID: 9570373
13668  Hirano S.  In vitro and in vivo cytotoxic effects of nitric oxide on metastatic cells  Cancer Lett  1997  115(1):57-62  PubMed ID: 9097979
6255  Nakajima M, Shinoda I, Samejima Y, Miyauchi H, Fukuwatari Y, Hayasawa H.  Kappa-casein suppresses melanogenesis in cultured pigment cells.  Pigment Cell Res  1996  9(5):235-9  PubMed ID: 9014209
5063  Kobori M, Sasaki Y, Shinohara K.  Suppressive effect of spinach extract on the formation of melanin in B16 mouse melanoma cells.  Cytotechnology  1994  14(2):119-22  PubMed ID: 22358759



Back Back Return Top Page