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Cell No. : Cell Name
RCB0556 : NIAS-PX-58  update : 2024/01/17
CommentPupal ovary hemocyte-like.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Year of deposit 1990
Animal _butterfly < Insect
Strain name swallow t.
Genus Papilio
Species xuthus Linne
Tissue pupal ovary
Classification other
Year of origin 1970
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z132
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
Temperature 28 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Reference information Reference 6
User's Publication 0


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Reference
1341  Hlromlchl MORIKAWA, Jun MITSUHASHI, and Yasuyuki YA  Interkingdom Electrofusion between Tobacco Mesophyll Protoplasts and Cultured Butterfly Cells   Plant Tissue Culture Letters  1988  5(2):90-92   
1045  Jun MITSUHASHI, Hajime INOUE  Obtainment of a Continuous Cell Line from the Larval Fat Bodies of the Mulberry Tiger Moth, Spilosoma imparilis (Lepidoptera:Arctiidae)  Applied Entomology and Zoology  1988  23:488-490    DOI: 10.1303/aez.23.488
1364  Mitsuhashi Jun  Fusion of Insect Cells  Biotechnology in Invertebrate Pathology and cell Culture  1987  387-400   
1346  Jun MITSUHASHI  Continuous Cultures of Insect Cell Lines in Media Free of Sera  Appl Ent Zool  1982  17:575-581   
1363  Mitsuhashi Jun  Requirement of amino acids by insect cell lines  Invertebrate Systems In vitro  1980  47-58   
1335  Jun Mitsuhashi  Utilization and requirements of amino acids by a cell line derived from the butterfly Papilio xuthus  Journal of Insect Physiology 2  1976  2:397-40    DOI: 10.1016/0022-1910(76)90009-3

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