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細胞番号 : 細胞名
RCB0555 : GCIY  update : 2021/02/26
細胞特性(Comment:英)Stomach cancer (Borrmann IV). Mucin, CA19-9, CEA, alphafetoprotein positive.
細胞特性(日)胃癌(Borrmann IV型)。ムチン産生性。CA19-9, CEA, alphafetoprotein陽性。
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Nozue, Mutsumi
樹立者 Nozue, Mutsumi
寄託日 1990
元の細胞 TKG0405
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Female
採取組織 stomach
病名 adenocarcinoma (Borrmann IV)
転移 Yes
細胞分類 cancer
細胞寿命 infinite
細胞形態 epithelial-like
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 15% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 56-122(50) : /59(4),60(11),61(19),62(3),63(2),66(1)/
文献情報 Reference(英) 3件
Reference(日) 1件
利用者成果(英) 50件
利用者成果(日) 0件

4757  Nozue M, Nishida M, Todoroki T, Fukao K, Tanaka M.  Selection of three out of 24 anti-cancer agents in poorly-differentiated gastric cancer cell lines, evaluated by the AUC/delta IC50 ratio.  Anticancer Drugs  1995  6(2):291-302  PubMed ID: 7795277
4758  Nozue M, Todoroki T, Fukao K.  5-fu dose-dependency in mtx/s-fu sequential therapy assessed by in-vitro assay using gastric-cancer cell-lines.  Oncol Rep  1995  2(5):737-40  PubMed ID: 21597807
1324  Nozue M, Nishida M, Todoroki T, Iwasaki Y  [Establishment and characterization of a human scirrhus type gastric cancer cell line, GCIY, producing CA19-9]  Hum Cell  1991  4:71-5  PubMed ID: 1653599

1706  野末睦, 西田正人, 轟健, 岩崎洋治  ヒト胃癌 : GCIY  生体の科学  1992  43:374 

13281  Kajioka H, Kagawa S, Ito A, Yoshimoto M, Sakamoto S, Kikuchi S, Kuroda S, Yoshida R, Umeda Y, Noma K, Tazawa H, Fujiwara T.  Targeting neutrophil extracellular traps with thrombomodulin prevents pancreatic cancer metastasis.  Cancer Lett  2021    PubMed ID: 33065249
20028  Ito A, Kagawa S, Sakamoto S, Kuwada K, Kajioka H, Yoshimoto M, Kikuchi S, Kuroda S, Yoshida R, Tazawa H, Fujiwara T.  Extracellular vesicles shed from gastric cancer mediate protumor macrophage differentiation.  BMC Cancer  2021  21(1):102  PubMed ID: 33509150
13337  Kim S, Ahn JM, Bae WJ, Han JH, Lee D.  Quantitation of ligand is critical for ligand-dependent MET signalling activation and determines MET-targeted therapeutic response in gastric cancer.  Gastric Cancer  2020    PubMed ID: 33164142
13504  Baatar S, Bai T, Yokobori T, Gombodorj N, Nakazawa N, Ubukata Y, Kimura A, Kogure N, Sano A, Sohda M, Sakai M, Tumenjargal A, Ogata K, Kuwano H, Shirabe K, Saeki H.  High RAD18 Expression is Associated with Disease Progression and Poor Prognosis in Patients with Gastric Cancer  Ann Surg Oncol  2020  27(11):4360-4368  PubMed ID: 32356270
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826
12415  Sakamoto S, Kagawa S, Kuwada K, Ito A, Kajioka H, Kakiuchi Y, Watanabe M, Kagawa T, Yoshida R, Kikuchi S, Kuroda S, Tazawa H, Fujiwara T.  Intraperitoneal cancer-immune microenvironment promotes peritoneal dissemination of gastric cancer  Oncoimmunology  2019  8(12):e1671760  PubMed ID: 31741772
10662  Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.  Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.  Biochem. Biophys. Res. Commun.  2018  495:1942-1947  PubMed ID: 29247652
4049  Taichi Yoshida, Takashi Maruyama, Masatomo Miura, Masahiro Inoue, Koji Fukuda, Kazuhiro Shimazu, Daiki Taguchi, Hiroaki Kanda, Masanobu Oshima, Yoshiharu Iwabuchi, Hiroyuki Shibata  Dietary intake of pyrolyzed deketene curcumin inhibits gastric carcinogenesis  Journal of Functional Foods  2018  50:192-200 
4188  Suzuki M, Yokobori T, Gombodorj N, Yashiro M, Turtoi A, Handa T, Ogata K, Oyama T, Shirabe K, Kuwano H.  High stromal transforming growth factor β-induced expression is a novel marker of progression and poor prognosis in gastric cancer.  J Surg Oncol  2018    PubMed ID: 30260476
4380  Yoshioka T, Shien K, Namba K, Torigoe H, Sato H, Tomida S, Yamamoto H, Asano H, Soh J, Tsukuda K, Nagasaka T, Fujiwara T, Toyooka S.  Antitumor activity of pan-HER inhibitors in HER2-positive gastric cancer.  Cancer Sci.  2018  109(4):1166-1176  PubMed ID: 29465762
10277  Masuda T, Song W, Nakanishi H, Lei W, Noor AM, Arai F.  Rare cell isolation and recovery on open-channel microfluidic chip.  PLoS ONE  2017  12:e0174937  PubMed ID: 28426707
9594  Shibahara-Sone H, Gomi A, Iino T, Kano M, Nonaka C, Watanabe O, Miyazaki K, Ohkusa T.  Living cells of probiotic Bifidobacterium bifidum YIT 10347 detected on gastric mucosa in humans.  Benef Microbes  2016  7:319-26  PubMed ID: 26925600
9928  Diaz-Lagares A, Crujeiras AB, Lopez-Serra P, Soler M, Setien F, Goyal A, Sandoval J, Hashimoto Y, Martinez-Cardús A, Gomez A, Heyn H, Moutinho C, Espada J, Vidal A, Paúles M, Galán M, Sala N, Akiyama Y, Martínez-Iniesta M, Farré L, Villanueva A, Gross M, Diederichs S, Guil S, Esteller M.  Epigenetic inactivation of the p53-induced long noncoding RNA TP53 target 1 in human cancer.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:E7535-E7544  PubMed ID: 27821766
3733  Nishikawaji T, Akiyama Y, Shimada S, Kojima K, Kawano T, Eishi Y, Yuasa Y, Tanaka S.  Oncogenic roles of the SETDB2 histone methyltransferase in gastric cancer.  Oncotarget  2016  7(41):67251-67265  PubMed ID: 27572307
5811  Maejima R et al  Enhanced expression of semaphorin 3E is involved in the gastric cancer development.  Int J Oncol  2016  49(3):887-94  PubMed ID: 27572291
5812  Hirashita Y et al  Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.  Cancer Sci  2016  107(12):1919-1928  PubMed ID: 27699948
12639  Yamashita K, Kuno A, Matsuda A, Ikehata Y, Katada N, Hirabayashi J, Narimatsu H, Watanabe M.  Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer  Gastric Cancer  2016  19(2):531-54  PubMed ID: 25840959
7357  Yamamoto S, Fei J, Okochi M, Shimizu K, Yusa A, Kondo N, Iwata H, Nakanishi H, Honda H.  Efficient capturing of circulating tumor cells using a magnetic capture column and a size-selective filter.  Bioprocess Biosyst Eng  2015  38:1693-704  PubMed ID: 25964183
5825  Ito A, Ito Y, Matsushima S, Tsuchida D, Ogasawara M, Hasegawa J, Misawa K, Kondo E, Kaneda N, Nakanishi H.  New whole-body multimodality imaging of gastric cancer peritoneal metastasis combining fluorescence imaging with ICG-labeled antibody and MRI in mice.  Gastric Cancer  2014  17(3):497-507  PubMed ID: 24288123
5302  Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.  Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.  Cancer Sci  2014  105(5):528-36  PubMed ID: 24612061
5845  Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.  Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.  PLoS One  2013  8(2):e56766  PubMed ID: 23451082
5847  Zhao L, Yasumoto K, Kawashima A, Nakagawa T, Takeuchi S, Yamada T, Matsumoto K, Yonekura K, Yoshie O, Yano S.  Paracrine activation of MET promotes peritoneal carcinomatosis in scirrhous gastric cancer.  Cancer Sci  2013  104(12):1640-6  PubMed ID: 24118504
6075  Murakami H, Nakanishi H, Tanaka H, Ito S, Misawa K, Ito Y, Ikehara Y, Kondo E, Kodera Y.  Establishment and characterization of novel gastric signet-ring cell and non signet-ring cell poorly differentiated adenocarcinoma cell lines with low and high malignant potential.  Gastric Cancer  2013  16(1):74-83  PubMed ID: 22450770
13662  Murakami H, Ito S, Tanaka H, Kondo E, Kodera Y, Nakanishi H.  Establishment of new intraperitoneal paclitaxel-resistant gastric cancer cell lines and comprehensive gene expression analysis  Anticancer Res  2013  33(10):4299-307  PubMed ID: 24122996
5853  Nakanishi H, Ito S, Matsui M, Ito Y, Misawa K, Kodera Y.  Noninvasive and real-time fluorescence imaging of peritoneal metastasis in nude mice.  Methods Mol Biol  2012  872:85-95  PubMed ID: 22700405
5856  Sato Y, Morimoto K, Kubo T, Yanagihara K, Seyama T.  High mannose-binding antiviral lectin PFL from Pseudomonas fluorescens Pf0-1 promotes cell death of gastric cancer cell MKN28 via interaction with α2-integrin.  PLoS One  2012  7(9):e45922  PubMed ID: 23029318
8118  Shirasawa Y, Shibahara-Sone H, Iino T, Ishikawa F.  Bifidobacterium bifidum BF-1 suppresses Helicobacter pylori-induced genes in human epithelial cells.  J. Dairy Sci.  2010  93:4526-34  PubMed ID: 20854986
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
5893  Hara M, Nakanishi H, Jun Q, Kanemitsu Y, Ito S, Mochizuki Y, Yamamura Y, Kodera Y, Tatematsu M, Hirai T, Kato T.  Comparative analysis of intraperitoneal minimal free cancer cells between colorectal and gastric cancer patients using quantitative RT-PCR: possible reason for rare peritoneal recurrence in colorectal cancer.  Clin Exp Metastasis  2007  24(3):179-89  PubMed ID: 17487561
5899  Yokoyama H, Ikehara Y, Kodera Y, Ikehara S, Yatabe Y, Mochizuki Y, Koike M, Fujiwara M, Nakao A, Tatematsu M, Nakanishi H.  Molecular basis for sensitivity and acquired resistance to gefitinib in HER2-overexpressing human gastric cancer cell lines derived from liver metastasis.  Br J Cancer  2006  95(11):1504-13  PubMed ID: 17088902
5906  Imura K, Ueda Y, Hayashi T, Itoh T, Shimizu K, Tamai H, Yano Y, Naito K, Kohara J, Nakane K, Matsuura Y, Takeda A, Takeda T, Kawai K, Yamagishi H.  Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique.  Int J Oncol  2006  29(3):531-9 
13874  Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique  Imura K, Ueda Y, Hayashi T, Itoh T, Shimizu K, Tamai H, Yano Y, Naito K, Kohara J, Nakane K, Matsuura Y, Takeda A, Takeda T, Kawai K, Yamagishi H.  Int J Oncol  2006  29(3):531-9  PubMed ID: 16865268
3077  Nakatsu N, Yoshida Y, Yamazaki K, Nakamura T, Dan S, Fukui Y, Yamori T  Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.  Mol Cancer Ther  2005  4:399-412  PubMed ID: 15767549
5910  Nakanishi H, Ito S, Mochizuki Y, Tatematsu M.  Evaluation of chemosensitivity of micrometastases with green fluorescent protein gene-tagged tumor models in mice.  Methods Mol Med  2005  2005;111:351-62  PubMed ID: 15911990
2807  Shida D, Kitayama J, Yamaguchi H, Hama K, Aoki J, Arai H, Yamashita H, Mori K, Sako A, Konishi T, Watanabe T, Sakai T, Suzuki R, Ohta H, Takuwa Y, Nagawa H  Dual mode regulation of migration by lysophosphatidic acid in human gastric cancer cells.  Exp Cell Res  2004  301:168-78  PubMed ID: 15530853
5397  Mochizuki Y, Nakanishi H, Kodera Y, Ito S, Yamamura Y, Kato T, Hibi K, Akiyama S, Nakao A, Tatematsu M.  TNF-alpha promotes progression of peritoneal metastasis as demonstrated using a green fluorescence protein (GFP)-tagged human gastric cancer cell line.  Clin Exp Metastasis  2004  21(1):39-47.  PubMed ID: 15065601
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956
5931  Terashima M, Fujiwara H, Takagane A, Abe K, Irinoda T, Nakaya T, Yonezawa H, Oyama K, Saito K, Kanzaki N, Ohtani S, Nemoto T, Hoshino Y, Kogure M, Gotoh M.  Prediction of sensitivity to fluoropyrimidines by metabolic and target enzyme activities in gastric cancer.  Gastric Cancer  2003  6 Suppl 1:71-81.  PubMed ID: 12775024
5932  Fujiwara 1, Terashima M, Irinoda T, Takagane A, Abe K, Nakaya T, Yonezawa H, Oyama K, Takahashi M, Saito K, Takechi T, Fukushima M, Shirasaka T.  Superior antitumour activity of S-1 in tumours with a high dihydropyrimidine dehydrogenase activity.  Eur J Cancer  2003  39(16):2387-94  PubMed ID: 14556932
5935  Dan S, Shirakawa M, Mukai Y, Yoshida Y, Yamazaki K, Kawaguchi T, Matsuura M, Nakamura Y, Yamori T.  Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines.  Cancer Sci  2003  94(12):1074-82  PubMed ID: 14662023
5941  Fujiwara H, Terashima M, Irinoda T, Takagane A, Abe K, Kashiwaba M, Oyama K, Takahashi M, Maesawa C, Saito K, Takechi T, Fukushima M.  Quantitative measurement of thymidylate synthase and dihydropyrimidine dehydrogenase mRNA level in gastric cancer by real-time RT-PCR.  Jpn J Cancer Res  2002  93(12):1342-50  PubMed ID: 12495474
3763  Takaishi S, Sawada M, Morita Y, Seno H, Fukuzawa H, Chiba T.  Identification of a novel alternative splicing of human FGF receptor 4: soluble-form splice variant expressed in human gastrointestinal epithelial cells.  Biochem Biophys Res Commun  2000  19;267(2):658-62  PubMed ID: 10631118
6386  Oji Y, Ogawa H, Tamaki H, Oka Y, Tsuboi A, Kim EH, Soma T, Tatekawa T, Kawakami M, Asada M, Kishimoto T, Sugiyama H.  Expression of the Wilms' tumor gene WT1 in solid tumors and its involvement in tumor cell growth.  Jpn J Cancer Res  1999  90(2):194-204  PubMed ID: 10189890
6409  Hassan S, Kinoshita Y, Kawanami C, Kishi K, Matsushima Y, Ohashi A, Funasaka Y, Okada A, Maekawa T, He-Yao W, Chiba T.  Expression of protooncogene c-kit and its ligand stem cell factor (SCF) in gastric carcinoma cell lines.  Dig Dis Sci  1998  43(1):8-14  PubMed ID: 9508539
5973  Tamura G, Sakata K, Nishizuka S, Maesawa C, Suzuki Y, Iwaya T, Terashima M, Saito K, Satodate R.  Analysis of the fragile histidine triad gene in primary gastric carcinomas and gastric carcinoma cell lines.  Genes Chromosomes Cancer  1997  20(1):98-102  PubMed ID: 9290961
2062  Wang, D S, Ueno Y, Oyamada H, Kojima S  Accumulation of NCC-CO-411 monoclonal antibody in human colorectal adenocarcinoma through binding of the released carcinoembryonic antigen in nude mice.  Biol Pharm Bull  1996  19:915-21  PubMed ID: 8839961
2177  Tamura G, Sakata K, Nishizuka S, Maesawa C, Suzuki Y, Iwaya T, Terashima M, Saito K, Satodate R  Inactivation of the E-cadherin gene in primary gastric carcinomas and gastric carcinoma cell lines  Jpn J Cancer Res  1996  87:1153-1159  PubMed ID: 9045944
2063  Wang, D., S., Ueno, Y., Oyamada, H., and Kojima, S.  Radioimmunoimaging of Human Colorectal Adenocarcinoma with a Monoclonal Antibody, NCC-CO-411, Against a Human Carcinoembryonic Antigen  Antibody, Immunoconjugates, and Radiopharmaceuticals  1995  8:129-143 
6407  Matsushima Y, Kinoshita Y, Nakata H, Inomoto-Naribayashi Y, Asahara M, Kawanami C, Nakamura A, Ito M, Matsui T, Fujiwara T, et al.  Gastrin receptor gene expression in several human carcinomas.  Jpn J Cancer Res  1994  85(8):819-24  PubMed ID: 7928627


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