Cell No. : Cell Name
RCB0554 : IA-LM
update : 2024/10/01
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Comment | Human cell line derived from lung cancer. Large cell carcinoma. Derived from skin metastasis. Transplantable in immuno-deficient mouse, and metastasis to lymph nodes can be observed. IA-5 (RCB0548) and IA-LM (RCB0554) were derived from the same patient. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Takiguchi, Yuichi
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Originator |
Miyamoto, Tadaaki & Takiguchi,Yuichi
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Year of deposit |
1990
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Animal |
_human
< Mammals
|
Genus |
Homo
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Species |
sapiens
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Race |
Japanese
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Gender |
Male
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Age at sampling |
68 years
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Tissue |
lung, skin meta
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Disease name |
lung carcinoma
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Metastatic ability |
Yes
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Metastatic tissue |
skin, pancreas
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Classification |
cancer
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Year of origin |
1989
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Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
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Morphology |
epithelial-like
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Cellosaurus(Expasy) |
CVCL_1302
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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HamF10 + 10% FBS
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Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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Virus (HIV) |
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(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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42-100(50) : /47(2),48(8),49(10),50(8),51(3),52(3)/
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STR(human) |
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OK
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Images |
deposit info | lot info |
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Reference information |
Reference |
0
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User's Publication |
5
|
User's Publication |
13773
Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.
Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites
Cancer Sci
2020
111(2):667-678
PubMed ID: 31828882
DOI: 10.1111/cas.14278
|
15895
Hallin J, Engstrom LD, Hargis L, Calinisan A, Aranda R, Briere DM, Sudhakar N, Bowcut V, Baer BR, Ballard JA, Burkard MR, Fell JB, Fischer JP, Vigers GP, Xue Y, Gatto S, Fernandez-Banet J, Pavlicek A, Velastagui K, Chao RC, Barton J, Pierobon M, Baldelli E, Patricoin EF 3rd, Cassidy DP, Marx MA, Rybkin II, Johnson ML, Ou SI, Lito P, Papadopoulos KP, Jänne PA, Olson P, Christensen JG.
The KRAS G12C Inhibitor MRTX849 Provides Insight toward Therapeutic Susceptibility of KRAS-Mutant Cancers in Mouse Models and Patients
Cancer Discov
2020
10(1):54-71
PubMed ID: 31658955
DOI: 10.1158/2159-8290.CD-19-1167
|
9581
Shimobaba S, Taga S, Akizuki R, Hichino A, Endo S, Matsunaga T, Watanabe R, Yamaguchi M, Yamazaki Y, Sugatani J, Ikari A.
Claudin-18 inhibits cell proliferation and motility mediated by inhibition of phosphorylation of PDK1 and Akt in human lung adenocarcinoma A549 cells.
Biochim. Biophys. Acta
2016
1863:1170-8
PubMed ID: 26919807
DOI: 10.1016/j.bbamcr.2016.02.015
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12718
Ikari A, Sato T, Watanabe R, Yamazaki Y, Sugatani J.
Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells
Biochim Biophys Acta
2012
1823(6):1110-8
PubMed ID: 22546605
DOI: 10.1016/j.bbamcr.2012.04.005
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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