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Cell No. : Cell Name
RCB0550 : Cle-H3^(neo+)  update : 2022/03/17
CommentpSV2neo-introduced G418-resistant Cle-H3 mutant.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takiguchi, Yuichi
Originator Miyamoto, Tadaaki
Year of deposit 1990
Another name H-3^(neo+)
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene MA CDNA, pSV2neo
Year of origin 1989
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T378
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS + 500μg/ml G418
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0

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2396  Takiguchi Y, Takahashi Y, Kuriyama T, Miyamoto T  NIH3T3 transfectant containing human K-ras oncogene shows enhanced metastatic activity after in vivo tumor growth or co-culture with fibroblasts.  Clin Exp Metastasis  1992  10:351-60  PubMed ID: 1505125

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