Cell No. : Cell Name
RCB0549 : Cle-H3
update : 2024/01/24
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| Comment | NIH3T3 transformant expressing human activated k-ras-2. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takiguchi, Yuichi
|
| Originator |
Miyamoto, Tadaaki
|
| Year of deposit |
1990
|
| Another name |
H-3, Cl-e
|
| Original cell |
NIH3T3
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NIH Swiss
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
MA cDNA
|
| Year of origin |
1986
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_F498
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
2
|
| Reference |
|
2398
Takahashi Y, Takiguchi Y, Kuriyama T, Miyamoto T
Lattices of type I collagen select invasive variants of K-ras oncogene-transfected NIH3T3 with less cellular fibronectin.
Clin Exp Metastasis
1998
16:149-57
PubMed ID: 9514096
DOI: 10.1023/a:1021937102996
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2397
Takiguchi Y, Kuriyama T, Miyamoto T
Evaluation of metastatic ability at specific times during primary tumor growth: a novel spontaneous metastasis assay.
Clin Exp Metastasis
1995
13:184-90
PubMed ID: 7750205
DOI: 10.1007/BF00132206
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2396
Takiguchi Y, Takahashi Y, Kuriyama T, Miyamoto T
NIH3T3 transfectant containing human K-ras oncogene shows enhanced metastatic activity after in vivo tumor growth or co-culture with fibroblasts.
Clin Exp Metastasis
1992
10:351-60
PubMed ID: 1505125
DOI: 10.1007/BF00058175
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