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Cell No. : Cell Name
RCB0549 : Cle-H3  update : 2020/09/24
CommentNIH3T3 transformant expressing human activated k-ras-2.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Takiguchi, Yuichi
Originator Miyamoto, Tadaaki
Year of deposit 1990
Another name H-3, Cl-e
Original cell NIH3T3
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene MA cDNA
Year of origin 1986
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
2398  Takahashi Y, Takiguchi Y, Kuriyama T, Miyamoto T  Lattices of type I collagen select invasive variants of K-ras oncogene-transfected NIH3T3 with less cellular fibronectin.  Clin Exp Metastasis  1998  16:149-57  PubMed ID: 9514096
2397  Takiguchi Y, Kuriyama T, Miyamoto T  Evaluation of metastatic ability at specific times during primary tumor growth: a novel spontaneous metastasis assay.  Clin Exp Metastasis  1995  13:184-90  PubMed ID: 7750205
2396  Takiguchi Y, Takahashi Y, Kuriyama T, Miyamoto T  NIH3T3 transfectant containing human K-ras oncogene shows enhanced metastatic activity after in vivo tumor growth or co-culture with fibroblasts.  Clin Exp Metastasis  1992  10:351-60  PubMed ID: 1505125

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User's Publication
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041



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