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Cell No. : Cell Name
RCB0549 : Cle-H3  update : 2024/01/24
CommentNIH3T3 transformant expressing human activated k-ras-2.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takiguchi, Yuichi
Originator Miyamoto, Tadaaki
Year of deposit 1990
Another name H-3, Cl-e
Original cell NIH3T3
Animal _mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene MA cDNA
Year of origin 1986
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_F498
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 2

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2398  Takahashi Y, Takiguchi Y, Kuriyama T, Miyamoto T  Lattices of type I collagen select invasive variants of K-ras oncogene-transfected NIH3T3 with less cellular fibronectin.  Clin Exp Metastasis  1998  16:149-57  PubMed ID: 9514096   DOI: 10.1023/a:1021937102996
2397  Takiguchi Y, Kuriyama T, Miyamoto T  Evaluation of metastatic ability at specific times during primary tumor growth: a novel spontaneous metastasis assay.  Clin Exp Metastasis  1995  13:184-90  PubMed ID: 7750205   DOI: 10.1007/BF00132206
2396  Takiguchi Y, Takahashi Y, Kuriyama T, Miyamoto T  NIH3T3 transfectant containing human K-ras oncogene shows enhanced metastatic activity after in vivo tumor growth or co-culture with fibroblasts.  Clin Exp Metastasis  1992  10:351-60  PubMed ID: 1505125   DOI: 10.1007/BF00058175

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User's Publication
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041   DOI: 10.2116/analsci.18P170
18003  Shrestha P, Sumitomo S, Lee CH, Nagahara K, Kamegai A, Yamanaka T, Takeuchi H, Kusakabe M, Mori M.  Tenascin: growth and adhesion modulation--extracellular matrix degrading function: an in vitro study  Eur J Cancer B Oral Oncol  1996  32B(2):106-13  PubMed ID: 8736172   DOI: 10.1016/0964-1955(95)00074-7

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