Cell No. : Cell Name
RCB0548 : IA-5
update : 2023/01/25
|
| Comment | Human cell line derived from lung cancer. Large cell carcinoma. Derived from skin metastasis. Transplantable in immuno-deficient mouse, but metastasis to lymph nodes is rarely observed. IA-5 (RCB0548) and IA-LM (RCB0554) were derived from the same patient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takiguchi, Yuichi
|
| Originator |
Miyamoto, Tadaaki, Takiguchi, Yuichi
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
68 years
|
| Tissue |
lung, skin meta
|
| Disease name |
lung carcinoma
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
skin, pancreas
|
| Classification |
cancer
|
| Year of origin |
1984
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_6658
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF10 + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
42-82(50) : /44(4),45(5),46(9),47(11),48(5),49(5)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
4
|
| Reference |
|
267
Hironori Ashinuma, Yuichi Takiguchi, Satoru Kitazono, Miyako Kitazono-Saitoh, Atsushi Kitamura, Yuji Tada, Katsushi Kurosu, Emiko Sakaida, Ikuo Sekine, Nobuhiro Tanabe, Koichiro Tatsumi
Abstract 2737: Anti-proliferative action of metformin on various types of human lung cancer cell lines
Cancer Research
2012
72:2737-2737
DOI: 10.1158/1538-7445.am2012-2737
|
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17471
Ashinuma H, Takiguchi Y, Kitazono S, Kitazono-Saitoh M, Kitamura A, Chiba T, Tada Y, Kurosu K, Sakaida E, Sekine I, Tanabe N, Iwama A, Yokosuka O, Tatsumi K.
Antiproliferative action of metformin in human lung cancer cell lines
Oncol Rep
2012
28(1):8-14
PubMed ID: 22576795
DOI: 10.3892/or.2012.1763
|
|
17470
Takiguchi Y, Miyamoto T, Nagao K, Kuriyama T.
Assessment of the homogeneous efficacy of carbon ions in the spread-out Bragg peak for human lung cancer cell lines
Radiat Med
2007
25(6):272-7
PubMed ID: 17634880
DOI: 10.1007/s11604-007-0134-6
|
| User's Publication |
|
19029
Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.
LRRN4 and UPK3B are markers of primary mesothelial cells
PLoS One
2011
6(10):e25391
PubMed ID: 21984916
DOI: 10.1371/journal.pone.0025391
|
|
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
|
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3113
Yoshimasu, Tatsuya, Sakurai, Teruhisa, Oura, Shoji, Hirai, Issei, Tanino, Hirokazu, Kokawa, Yozo, Naito, Yasuaki, Okamura, Yoshitaka, Ota, Ichiro, Tani, Naoyuki, Matsuura, Nariaki
Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.
Cancer Sci
2004
95:142-8
PubMed ID: 14965364
DOI: 10.1111/j.1349-7006.2004.tb03195.x
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3134
Yoshimasu, T. et al
Integrins α2β1,α5β1 and αvβ5 are related to tumor growth and metastasis of non-small cell cancer
Japanese Journal of Lung Cancer
2001
41:111-115
DOI: 10.2482/haigan.41.111
|
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