Cell No. : Cell Name RCB0548 : IA-5  update : 2023/01/25
Comment			Human cell line derived from lung cancer. Large cell carcinoma. Derived from skin metastasis. Transplantable in immuno-deficient mouse, but metastasis to lymph nodes is rarely observed. IA-5 (RCB0548) and IA-LM (RCB0554) were derived from the same patient.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takiguchi, Yuichi
		Originator	Miyamoto, Tadaaki, Takiguchi, Yuichi
		Year of deposit	1990
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	68 years
		Tissue	lung, skin meta
		Disease name	lung carcinoma
		Metastatic ability	Yes
		Metastatic tissue	skin, pancreas
		Classification	cancer
		Year of origin	1984
		Memo_1	Mycoplasma was eliminated with MC-210.
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_6658
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF10 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		42-82(50) : /44(4),45(5),46(9),47(11),48(5),49(5)/
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	3

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Reference

267  Hironori Ashinuma, Yuichi Takiguchi, Satoru Kitazono, Miyako Kitazono-Saitoh, Atsushi Kitamura, Yuji Tada, Katsushi Kurosu, Emiko Sakaida, Ikuo Sekine, Nobuhiro Tanabe, Koichiro Tatsumi  Abstract 2737: Anti-proliferative action of metformin on various types of human lung cancer cell lines  Cancer Research  2012  72:2737-2737    DOI: 10.1158/1538-7445.am2012-2737

17471  Ashinuma H, Takiguchi Y, Kitazono S, Kitazono-Saitoh M, Kitamura A, Chiba T, Tada Y, Kurosu K, Sakaida E, Sekine I, Tanabe N, Iwama A, Yokosuka O, Tatsumi K.  Antiproliferative action of metformin in human lung cancer cell lines  Oncol Rep  2012  28(1):8-14  PubMed ID: 22576795   DOI: 10.3892/or.2012.1763

17470  Takiguchi Y, Miyamoto T, Nagao K, Kuriyama T.  Assessment of the homogeneous efficacy of carbon ions in the spread-out Bragg peak for human lung cancer cell lines  Radiat Med  2007  25(6):272-7  PubMed ID: 17634880   DOI: 10.1007/s11604-007-0134-6

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User's Publication

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

3113  Yoshimasu, Tatsuya, Sakurai, Teruhisa, Oura, Shoji, Hirai, Issei, Tanino, Hirokazu, Kokawa, Yozo, Naito, Yasuaki, Okamura, Yoshitaka, Ota, Ichiro, Tani, Naoyuki, Matsuura, Nariaki  Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.  Cancer Sci  2004  95:142-8  PubMed ID: 14965364   DOI: 10.1111/j.1349-7006.2004.tb03195.x

3134  Yoshimasu, T. et al  Integrins α2β1,α5β1 and αvβ5 are related to tumor growth and metastasis of non-small cell cancer   Japanese Journal of Lung Cancer  2001  41:111-115    DOI: 10.2482/haigan.41.111