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Cell No. : Cell Name
RCB0545 : OHTA  update : 2022/03/17
CommentMouse embryonic carcinoma
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamada, Takeshi
Originator Ohta, K.
Year of deposit 1990
Animal mouse < Mammals
Strain name ICR
Age at sampling embryo
Tissue embryo/fetus, whole
Disease name embryonic carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4665
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 39-41(50) : 39(2),40(41),41(7)
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
8854  Maccarrone G, Kolb N, Teplytska L, Birg I, Zollinger R, Holsboer F, Turck CW.  Phosphopeptide enrichment by IEF.  Electrophoresis  2006  27:4585-95  PubMed ID: 17066382   DOI: 10.1002/elps.200600145



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