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Cell No. : Cell Name
RCB0544 : MBT-2  update : 2022/03/17
CommentA mouse cell line derived from bladder cancer.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Akaza, Hideyuki
Year of deposit 1990
Animal mouse < Mammals
Tissue bladder
Disease name transitional cell carcinoma
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4660
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 8
User's Publication 9


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Reference
4756  Matzkin H, Rangel MC, Soloway MS.  In vitro study of the effect of hyperthermia on normal bladder cell line and on five different transitional cell carcinoma cell lines.  J Urol  1992  147(6):1671-4  PubMed ID: 1593718   DOI: 10.1016/s0022-5347(17)37675-9
4755  Brausi M1, Blatnik A, Soloway MS.  Dose response relationship of methotrexate in combination with cisplatin in murine bladder cancer.  Urology  1990  35(3):253-6  PubMed ID: 2316090   DOI: 10.1016/0090-4295(90)80044-n
4754  Hickey DP1, Brausi M, Blatnik AF, Soloway MS.  Comparison of single agent cisplatin and cisplatin containing combinations in treatment of murine bladder cancer.  Urology  1987  30(5):484-7  PubMed ID: 3118551   DOI: 10.1016/0090-4295(87)90388-8
4753  Niell HB, Herrod HG, Blatnik AF, Soloway MS.  In vitro and in vivo activity of two second generation platinum analogs in murine bladder cancer.  J Urol  1985  134(2):399-402  PubMed ID: 3894702   DOI: 10.1016/s0022-5347(17)47186-2
4752  Nag S, Blatnik A, Soloway M.  Enhancement of high intensity Iodine-125 brachytherapy by cis-platinum in a murine bladder tumor model.  J Urol  1984  131(6):1225-8  PubMed ID: 6539386   DOI: 10.1016/s0022-5347(17)50877-0
4751  Crabtree WN, Soloway MS, Matheny RB Jr, Murphy WM.  Metastatic characteristics of four FANFT-induced murine bladder tumors.  Urology  1983  22(5):529-31  PubMed ID: 6649210   DOI: 10.1016/0090-4295(83)90235-2
4750  Mickey DD, Mickey GH, Murphy WM, Niell HB, Soloway MS.  In vitro characterization of four N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT) induced mouse bladder tumors.  J Urol  1982  127(6):1233-7  PubMed ID: 7087044   DOI: 10.1016/s0022-5347(17)54305-0
1443  Soloway, M S  Intravesical and systemic chemotherapy of murine bladder cancer.  Cancer Res  1977  37:2918-29  PubMed ID: 406041  

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User's Publication
13186  Huetter J, Gritzan U, Gutcher I, Doecke WD, Luetke-Eversloh MV, Golfier S, Roider HG, Frisk AL, Hunter J, Pow A, Drake A, Levine Z, Levy O, Azulay M, Barbiro I, Cojocaru G, Vaknin I, Kreft B, Roese L.  Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2).  Cancer Immunol Res  2020    PubMed ID: 32312711   DOI: 10.1158/2326-6066.CIR-19-0321
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979   DOI: 10.1128/MCB.00154-19
14361  Sen T, Della Corte CM, Milutinovic S, Cardnell RJ, Diao L, Ramkumar K, Gay CM, Stewart CA, Fan Y, Shen L, Hansen RJ, Strouse B, Hedrick MP, Hassig CA, Heymach JV, Wang J, Byers LA.  Combination Treatment of the Oral CHK1 Inhibitor, SRA737, and Low-Dose Gemcitabine Enhances the Effect of Programmed Death Ligand 1 Blockade by Modulating the Immune Microenvironment in SCLC  J Thorac Oncol  2019  14(12):2152-2163  PubMed ID: 31470128   DOI: 10.1016/j.jtho.2019.08.009
7121  Kato S, Mori S, Kodama T.  A Novel Treatment Method for Lymph Node Metastasis Using a Lymphatic Drug Delivery System with Nano/Microbubbles and Ultrasound.  J Cancer  2015  6:1282-94  PubMed ID: 26640589   DOI: 10.7150/jca.13028
15665  Nakamura T, Fukiage M, Higuchi M, Nakaya A, Yano I, Miyazaki J, Nishiyama H, Akaza H, Ito T, Hosokawa H, Nakayama T, Harashima H.  Nanoparticulation of BCG-CWS for application to bladder cancer therapy  J Control Release  2014  176:44-53  PubMed ID: 24389133   DOI: 10.1016/j.jconrel.2013.12.027
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014
8911  FAN You-ming, GAO Yu-qi, HUANG Jian, LIAO Wei-gong, CAI Ming-chun  Screening and identification of differentially expressed genes in hippocampus of mice during hypobaric hypoxic delayed preconditioning  Chinese Journal of Pathophysiology  2006  22:342-346   
9046  Shin-ichi Kohno 1, Chenhong Luo, Fumi Goshima, Yukihiro Nishiyama, Tetsutaro Sata, Yoshinari Ono  Herpes Simplex Virus Type 1 Mutant HF10 Oncolytic Viral Therapy for Bladder Cancer  Urology  2005  66 (5), 1116-21  PubMed ID: 16286150   DOI: 10.1016/j.urology.2005.05.041
2669  Shibata MA, Horiguchi T, Morimoto J, Otsuki Y  Massive apoptotic cell death in chemically induced rat urinary bladder carcinomas following in situ HSVtk electrogene transfer.  J Gene Med  2003  5:219-31  PubMed ID: 12666188   DOI: 10.1002/jgm.335


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