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Cell No. : Cell Name
RCB0540 : HAL-01  update : 2022/03/17
CommentAcute lymphatic leukemia
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Tokyo Medical University

6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023 Japan
Fax. +81-3-5381-6651
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohyashiki, Kazuma
Originator Ohyashiki, Kazuma
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 17 years
Tissue blood
Disease name acute lymphatic leukemia
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1242
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 40-47(50) : /42(3),43(3),44(3),45(14),46(21),47(4)
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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1325  Ohyashiki K, Fujieda H, Miyauchi J, Ohyashiki, J H, Tauchi T, Saito M, Nakazawa S, Abe K, Yamamoto K, Clark, S C, et al.  Establishment of a novel heterotransplantable acute lymphoblastic leukemia cell line with a t(17;19) chromosomal translocation the growth of which is inhibited by interleukin-3.  Leukemia  1991  5:322-31  PubMed ID: 2027299  

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User's Publication
8398  Ikawa Y, Sugimoto N, Koizumi S, Yachie A, Saikawa Y.  Dense methylation of types 1 and 2 regulatory regions of the CD10 gene promoter in infant acute lymphoblastic leukemia with MLL/AF4 fusion gene.  J. Pediatr. Hematol. Oncol.  2010  32:4-10  PubMed ID: 20051780   DOI: 10.1097/MPH.0b013e3181c29c3c
2240  Matsuo,Y., Okochi, A., Iimura, E., Ohno, T.  Variable Numbers OF Tandem Repeat (VNTR) Amplification by Polymerase ChainReaction For Identification Of Cell Lines  3rd Internet World Congress on Biomedical Sciences in RIKEN 1996  1997     
2119  Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura and Tadao Ohno  Identification of Cell Lines with Variabel Numbers of Tandem Repeat(VNTR) Amplified by Polymerase Chain Reaction  Tiss Cult Res Commun  1996  15:211-219    DOI: 10.11418/jtca1981.15.4_21

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