Cell No. : Cell Name
RCB0538 : PU5-18
update : 2023/06/30
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Comment | Mouse lymphoid tumor, macrophage like. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85051501 prior to order. |
Comment from the depositor | |
Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85051501 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
Year of deposit |
1990
|
Animal |
_mouse
< Mammals
|
Tissue |
lymphoid tumor
|
Classification |
cancer
|
History |
overseas, ECACC
|
|
Lifespan |
infinite
|
Morphology |
other
|
Cellosaurus(Expasy) |
CVCL_2696
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
RPMI1640 + 10% FBS
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
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Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma |
|
(-)
|
SSLP(mouse) |
|
OK
|
Isozyme |
|
LD, NP
|
Chromosome mode |
|
39-81(50) : 39(3),40(16),41(16),42(10),43(2),78(1)/
|
Images |
deposit info | lot info |
|
|
Reference information |
Reference |
3
|
User's Publication |
5
|
User's Publication |
15028
Inaba H, Yoshigai E, Okuyama T, Murakoshi M, Sugiyama K, Nishino H, Nishizawa M.
Antipyretic analgesic drugs have different mechanisms for regulation of the expression of inducible nitric oxide synthase in hepatocytes and macrophages
Nitric Oxide
2015
44:61-70
PubMed ID: 25499030
DOI: 10.1016/j.niox.2014.12.001
|
2637
Kasahara M, Watanabe Y, Sumasu M, Nagata T
A family of MHC class I-like genes located in the vicinity of the mouse leukocyte receptor complex.
Proc Natl Acad Sci U S A
2002
99:13687-92
PubMed ID: 12370446
DOI: 10.1073/pnas.212375299
|
5393
Harada M, Imai J, Okuno S, Suzuki T.
Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.
J Control Release
2000
69(3):389-97
PubMed ID: 11102679
DOI: 10.1016/s0168-3659(00)00320-5
|
15405
Keiichiro Yoshida, Michio Ono, Hajime Sawada
Lipopolysaccharide-induced vacuoles in macrophages: Their origin is plasma membrane-derived organelles and endoplasmic reticulum, but not lysosomes
Journal of Endotoxin Research
1999
5:127-137
DOI: 10.1177/09680519990050030601
|
1948
Tomita Mitsuko
Estimation and Analysis of Relative Amounts of Murine Attachmin Expressed in Cell Membranes of a Monocyte/Macrophage Lineage
Bioiamges
1995
3:107-112
|