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Cell No. : Cell Name
RCB0538 : PU5-18  update : 2023/06/30
CommentMouse lymphoid tumor, macrophage like. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85051501 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85051501 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 1990
Animal _mouse < Mammals
Tissue lymphoid tumor
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2696
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 39-81(50) : 39(3),40(16),41(16),42(10),43(2),78(1)/
deposit info
lot info
Reference information Reference 3
User's Publication 5

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1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922  
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528

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User's Publication
15028  Inaba H, Yoshigai E, Okuyama T, Murakoshi M, Sugiyama K, Nishino H, Nishizawa M.  Antipyretic analgesic drugs have different mechanisms for regulation of the expression of inducible nitric oxide synthase in hepatocytes and macrophages  Nitric Oxide  2015  44:61-70  PubMed ID: 25499030   DOI: 10.1016/j.niox.2014.12.001
2637  Kasahara M, Watanabe Y, Sumasu M, Nagata T  A family of MHC class I-like genes located in the vicinity of the mouse leukocyte receptor complex.  Proc Natl Acad Sci U S A  2002  99:13687-92  PubMed ID: 12370446   DOI: 10.1073/pnas.212375299
5393  Harada M, Imai J, Okuno S, Suzuki T.  Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.  J Control Release  2000  69(3):389-97  PubMed ID: 11102679   DOI: 10.1016/s0168-3659(00)00320-5
15405  Keiichiro Yoshida, Michio Ono, Hajime Sawada  Lipopolysaccharide-induced vacuoles in macrophages: Their origin is plasma membrane-derived organelles and endoplasmic reticulum, but not lysosomes  Journal of Endotoxin Research  1999  5:127-137    DOI: 10.1177/09680519990050030601
1948  Tomita Mitsuko  Estimation and Analysis of Relative Amounts of Murine Attachmin Expressed in Cell Membranes of a Monocyte/Macrophage Lineage  Bioiamges  1995  3:107-112   

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