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Cell No. : Cell Name
RCB0537 : JM  update : 2019/12/11
CommentHuman T cell line with the ability to grow HIV, the same patient as Jurkat. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86010201 prior to order.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86010201 prior to order. In addition, regarding commercial use and use for patent filing the users of this cell line must contact RIKEN BRC prior to order.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue blood
Case history acute T cell leukemia
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 6-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-93(50) : 45(7),46(29),47(8),90(1),92(4),93(1)
Surface antigen CD3(+), CD4(+), CD8(+)
Images
deposit info
lot info
Reference information Reference 3
User's Publication 3


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Reference
5071  Drexler HG, Gaedicke G, Minowada J.  T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.  Blut  1987  54(2):79-87  PubMed ID: 3493047
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
902  Schneider U, Schwenk HU, Bornkamm G.  Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.  Int J Cancer  1977  19(5):621-6  PubMed ID: 68013

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User's Publication
6957  Iino T, Okano K, Lee SW, Yamakawa T, Hagihara H1, Hong ZY, Maeno T, Kasai Y, Sakuma S, Hayakawa T, Arai F, Ozeki Y, Goda K, Hosokawa Y.  High-speed microparticle isolation unlimited by Poisson statistics.  Lab Chip  2019  19(16):2669-2677  PubMed ID: 31332412
10788  Koshizuka T, Kobayashi T, Ishioka K, Suzutani T.  Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases.  Sci Rep  2018  8:4447  PubMed ID: 29535361
2431  Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y  Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.  Infect Immun  1996  64:3093-100  PubMed ID: 8757839



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