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Cell No. : Cell Name
RCB0537 : JM  update : 2024/01/17
CommentHuman cell line derived from T-cell leukemia. Isolated from the same patient as “Jurkat”. Has the ability to grow HIV. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86010201 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86010201 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue blood
Disease name acute T cell leukemia
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0065
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 6-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-93(50) : 45(7),46(29),47(8),90(1),92(4),93(1)
Surface antigen CD3(+), CD4(+), CD8(+)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 4


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Reference
5071  Drexler HG, Gaedicke G, Minowada J.  T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.  Blut  1987  54(2):79-87  PubMed ID: 3493047   DOI: 10.1007/bf00321034
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
902  Schneider U, Schwenk HU, Bornkamm G.  Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.  Int J Cancer  1977  19(5):621-6  PubMed ID: 68013   DOI: 10.1002/ijc.2910190505

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User's Publication
21454  Liu S, Mochizuki M, Suzuki Y, Takemasa E, Yano A, Imai M, Mogi M.  Dietary leucine supplementation restores T-cell mitochondrial respiration and regulates T-lineage differentiation in denervation-induced sarcopenic mice.  J Nutr Biochem  2023  109508  PubMed ID: 37898392   DOI: 10.1016/j.jnutbio.2023.109508
6957  Iino T, Okano K, Lee SW, Yamakawa T, Hagihara H1, Hong ZY, Maeno T, Kasai Y, Sakuma S, Hayakawa T, Arai F, Ozeki Y, Goda K, Hosokawa Y.  High-speed microparticle isolation unlimited by Poisson statistics.  Lab Chip  2019  19(16):2669-2677  PubMed ID: 31332412   DOI: 10.1039/c9lc00324j
10788  Koshizuka T, Kobayashi T, Ishioka K, Suzutani T.  Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases.  Sci Rep  2018  8:4447  PubMed ID: 29535361   DOI: 10.1038/s41598-018-22682-2
2431  Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y  Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.  Infect Immun  1996  64:3093-100  PubMed ID: 8757839   DOI: 10.1128/IAI.64.8.3093-3100.1996



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