Cell No. : Cell Name
RCB0536 : STO
update : 2023/05/25
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| Comment | Tioguanine- and ouabain-resistant and HAT-sensitive fibroblasts. Suitable for ES cell feeder layer. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86032003 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86032003 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
| Year of deposit |
1990
|
| Animal |
_mouse
< Mammals
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
mutant
|
| History |
overseas, ECACC
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3420
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS + 0.1mM NEAA
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
50-108(50) : 50(5),51(2),52(11),53(13),54(13),55(1)/
|
| Images |
deposit info | lot info |
|
|
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| Reference information |
Reference |
4
|
| User's Publication |
10
|
| Reference |
|
2202
Talbot, N C, Rexroad, C E Jr, Powell, A M, Pursel, V G, Caperna, T J, Ogg, S L, Nel, N D
A continuous culture of pluripotent fetal hepatocytes derived from the 8-day epiblast of the pig.
In Vitro Cell Dev Biol Anim
1994
30A:843-50
PubMed ID: 7534591
DOI: 10.1007/BF02639394
|
|
4737
Martin GR, Wiley LM, Damjanov I.
The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells.
Dev Biol
1977
61(2):230-44
PubMed ID: 590624
DOI: 10.1016/0012-1606(77)90294-9
|
|
1293
Martin, G R, Evans, M J
Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.
Proc Natl Acad Sci U S A
1975
72:1441-5
PubMed ID: 1055416
DOI: 10.1073/pnas.72.4.1441
|
|
4736
Gail R.Martin, Martin J.Evans
Multiple differentiation of clonal teratocarcinoma stem cells following embryoid body formation in vitro
Cell
1975
6:467-474
DOI: 10.1016/0092-8674(75)90035-5
|
| User's Publication |
|
11425
Sekai M, Wang J, Minato N, Hamazaki Y.
An improved clonogenic culture method for thymic epithelial cells.
J. Immunol. Methods
2019
PubMed ID: 30738040
DOI: 10.1016/j.jim.2019.02.003
|
|
11739
Sekai M, Wang J, Hamazaki Y.
Clonogenic Culture of Mouse Thymic Epithelial Cells.
Methods Mol. Biol.
2019
PubMed ID: 31396938
DOI: 10.1007/978-1-4939-9728-2_15
|
|
10040
Kim N, Minami N, Yamada M, Imai H.
Immobilized pH in culture reveals an optimal condition for somatic cell reprogramming and differentiation of pluripotent stem cells.
Reprod. Med. Biol.
2017
16:58-66
PubMed ID: 29259452
DOI: 10.1002/rmb2.12011
|
|
15693
Sakuragi M, Tsuzuki S, Obuse S, Wada A, Matoba K, Kubo I, Ito Y.
A photoimmobilizable sulfobetaine-based polymer for a nonbiofouling surface
Mater Sci Eng C Mater Biol Appl
2010
30(2):316-322
PubMed ID: 30011623
DOI: 10.1016/j.msec.2009.11.006
|
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8613
Makoto Sakuragi1, Saki Tsuzuki1, Hirokazu Hasuda2, Akira Wada1, Kenji Matoba1,3, Izumi Kubo3, Yoshihiro Ito1,2,*
Synthesis of a photoimmobilizable histidine polymer for surface modification
J Applied Polymer Science
2009
112:315-319
DOI: 10.1002/app.29427
|
|
8657
Ito A, Jitsunobu H, Kawabe Y, Ijima H, Kamihira M.
Magnetic separation of cells in coculture systems using magnetite cationic liposomes.
Tissue Eng Part C Methods
2009
15:413-23
PubMed ID: 19196153
DOI: 10.1089/ten.tec.2008.0496
|
|
8033
Takahashi J, Takagi Y, Saiki H.
Transplantation of embryonic stem cell-derived dopaminergic neurons in MPTP-treated monkeys
Methods Mol Biol
2009
482:199-212
PubMed ID: 19089358
DOI: 10.1007/978-1-59745-060-7_13
|
|
3402
Tsutsui M, Ogawa S, Inada Y, Tomioka E, Kamiyoshi A, Tanaka S, Kishida T, Nishiyama M, Murakami M, Kuroda J, Hashikura Y, Miyagawa S, Satoh F, Shibata N, Tagawa Y.
Characterization of cytochrome P450 expression in murine embryonic stem cell-derived hepatic tissue system.
Drug Metab. Dispos.
2006
34:696-701
PubMed ID: 16415121
DOI: 10.1124/dmd.105.007674
|
|
6984
Takahashi M, Haruta M.
Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro.
Methods Mol Biol
2006
330:417-29
PubMed ID: 16846040
DOI: 10.1385/1-59745-036-7:417
|
|
3189
Ogawa S, Tagawa Y, Kamiyoshi A, Suzuki A, Nakayama J, Hashikura Y, Miyagawa S.
Crucial roles of mesodermal cell lineages in a murine embryonic stem cell-derived in vitro liver organogenesis system.
Stem Cells
2005
23:903-13
PubMed ID: 16043458
DOI: 10.1634/stemcells.2004-0295
|
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