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Cell No. : Cell Name
RCB0536 : STO  update : 2019/12/16
CommentTioguanine- and ouabain-resistant and HAT-sensitive fibroblasts. Suitable for ES cell feeder layer. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 86032003 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86032003 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1990
Animal mouse < Mammals
Age at sampling embryo
Tissue embryo
Classification mutant
History overseas, ECACC
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 50-108(50) : 50(5),51(2),52(11),53(13),54(13),55(1)/
deposit info
lot info
Reference information Reference 4
User's Publication 9

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2202  Talbot, N C, Rexroad, C E Jr, Powell, A M, Pursel, V G, Caperna, T J, Ogg, S L, Nel, N D  A continuous culture of pluripotent fetal hepatocytes derived from the 8-day epiblast of the pig.  In Vitro Cell Dev Biol Anim  1994  30A:843-50  PubMed ID: 7534591
4737  Martin GR, Wiley LM, Damjanov I.  The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells.  Dev Biol  1977  61(2):230-44  PubMed ID: 590624
1293  Martin, G R, Evans, M J  Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.  Proc Natl Acad Sci U S A  1975  72:1441-5  PubMed ID: 1055416
4736  Gail R.Martin, Martin J.Evans  Multiple differentiation of clonal teratocarcinoma stem cells following embryoid body formation in vitro  Cell  1975  6:467-474 

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User's Publication
11425  Sekai M, Wang J, Minato N, Hamazaki Y.  An improved clonogenic culture method for thymic epithelial cells.  J. Immunol. Methods  2019    PubMed ID: 30738040
11739  Sekai M, Wang J, Hamazaki Y.  Clonogenic Culture of Mouse Thymic Epithelial Cells.  Methods Mol. Biol.  2019    PubMed ID: 31396938
10040  Kim N, Minami N, Yamada M, Imai H.  Immobilized pH in culture reveals an optimal condition for somatic cell reprogramming and differentiation of pluripotent stem cells.  Reprod. Med. Biol.  2017  16:58-66  PubMed ID: 29259452
8613  Makoto Sakuragi1, Saki Tsuzuki1, Hirokazu Hasuda2, Akira Wada1, Kenji Matoba1,3, Izumi Kubo3, Yoshihiro Ito1,2,*  Synthesis of a photoimmobilizable histidine polymer for surface modification  J Applied Polymer Science  2009  112:315-319 
8657  Ito A, Jitsunobu H, Kawabe Y, Ijima H, Kamihira M.  Magnetic separation of cells in coculture systems using magnetite cationic liposomes.  Tissue Eng Part C Methods  2009  15:413-23  PubMed ID: 19196153
8033  Takahashi J, Takagi Y, Saiki H.  Transplantation of embryonic stem cell-derived dopaminergic neurons in MPTP-treated monkeys  Methods Mol Biol  2009  482:199-212  PubMed ID: 19089358
3402  Tsutsui M, Ogawa S, Inada Y, Tomioka E, Kamiyoshi A, Tanaka S, Kishida T, Nishiyama M, Murakami M, Kuroda J, Hashikura Y, Miyagawa S, Satoh F, Shibata N, Tagawa Y.  Characterization of cytochrome P450 expression in murine embryonic stem cell-derived hepatic tissue system.  Drug Metab. Dispos.  2006  34:696-701  PubMed ID: 16415121
6984  Takahashi M, Haruta M.  Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro.  Methods Mol Biol  2006  330:417-29  PubMed ID: 16846040
3189  Ogawa S, Tagawa Y, Kamiyoshi A, Suzuki A, Nakayama J, Hashikura Y, Miyagawa S.  Crucial roles of mesodermal cell lineages in a murine embryonic stem cell-derived in vitro liver organogenesis system.  Stem Cells  2005  23:903-13  PubMed ID: 16043458

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