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Cell No. : Cell Name
RCB0533 : Y1  update : 2022/08/02
CommentMouse steroid hormone secreting adrenal cortex cell line. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85062807 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85062807 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1990
Animal mouse < Mammals
Gender Male
Tissue adrenal cortex
Classification other
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0585
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD, PepB
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
1734  Yasumura Y, Buonassisi V, Sato G  Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype.  Cancer Res  1966  26:529-35  PubMed ID: 5930699  

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User's Publication
13458  Kitaoka K, Kawata S, Yoshida T, Kadoriku F, Kitamura M.  Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line  PLoS One  2016  11(4):e0154167  PubMed ID: 27100201   DOI: 10.1371/journal.pone.0154167
15621  Xu Y, Takeda S, Nakata T, Noda Y, Tanaka Y, Hirokawa N.  Role of KIFC3 motor protein in Golgi positioning and integration  J Cell Biol  2002  158(2):293-303  PubMed ID: 12135985   DOI: 10.1083/jcb.200202058



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