Cell No. : Cell Name RCB0533 : Y1  update : 2026/06/19
Comment			Mouse steroid hormone secreting adrenal cortex cell line. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85062807 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85062807 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). Regarding commercial use or use related to patent applications, please first contact the RIKEN BRC at cellbank.brc@riken.jp.
	Basic information	Depositor	ECACC
		Year of deposit	1990
		Animal	_mouse < Mammals
		Gender	Male
		Tissue	adrenal cortex
		Classification	other
		History	overseas, ECACC
		Lifespan	infinite
		Morphology	epithelial-like
		SDS	SDS_DMSO(Japanese)
		Cellosaurus(Expasy)	CVCL_0585
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP, G6PD, PepB
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	5

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Reference

1734  Yasumura Y, Buonassisi V, Sato G  Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype.  Cancer Res  1966  26:529-35  PubMed ID: 5930699

33  BUONASSISI V, SATO G, COHEN AI.  Hormone-producing cultures of adrenal and pituitary tumor origin  Proc Natl Acad Sci U S A  1962  48(7):1184-90  PubMed ID: 13874682   DOI: 10.1073/pnas.48.7.1184

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User's Publication

13458  Kitaoka K, Kawata S, Yoshida T, Kadoriku F, Kitamura M.  Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line  PLoS One  2016  11(4):e0154167  PubMed ID: 27100201   DOI: 10.1371/journal.pone.0154167

17350  Sugawara T, Nomura E, Nakajima A, Sakuragi N.  Characterization of binding between SF-1 and Sp1: predominant interaction of SF-1 with the N-terminal region of Sp1  J Endocrinol Invest  2004  27(2):133-41  PubMed ID: 15129808   DOI: 10.1007/BF03346258

15621  Xu Y, Takeda S, Nakata T, Noda Y, Tanaka Y, Hirokawa N.  Role of KIFC3 motor protein in Golgi positioning and integration  J Cell Biol  2002  158(2):293-303  PubMed ID: 12135985   DOI: 10.1083/jcb.200202058

601  Sugawara T, Abe S, Sakuragi N, Fujimoto Y, Nomura E, Fujieda K, Saito M, Fujimoto S.  RIP 140 modulates transcription of the steroidogenic acute regulatory protein gene through interactions with both SF-1 and DAX-1  Endocrinology  2001  142(8):3570-7  PubMed ID: 11459805   DOI: 10.1210/endo.142.8.8309

617  Sugawara T, Nomura E, Sakuragi N, Fujimoto S.  The effect of the arylhydrocarbon receptor on the human steroidogenic acute regulatory gene promoter activity  J Steroid Biochem Mol Biol  2001  78(3):253-60  PubMed ID: 11595506   DOI: 10.1016/s0960-0760(01)00100-5