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Cell No. : Cell Name
RCB0531 : TtT/M-87  update : 2022/03/17
CommentMouse cell line derived from pituitary tumor (thyrotropin-producing tumor). Macrophage-like cells. Dependent on conditioned medium of L929 cells. Conditioned medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1990
Animal mouse < Mammals
Gender Unknown
Tissue pituitary
Disease name thyrotropic pituitary tumor
Classification cancer
Year of origin 1985
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U804
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 15% FBS + 20% (RCB1451 L929 conditioned medium)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
4577  Mitsuishi T, Akuzawa Y, Sato S, Rui J, Kodama K, Inoue K, Kurashige S.  Immunological properties of TtT/M-87 cell line established from murine pituitary tumor-associated macrophages.  Microbiol Immunol  1993  37(12):943-51  PubMed ID: 8133800   DOI: 10.1111/j.1348-0421.1993.tb01728.x
1290  Inoue, K., Ito, A., Kubota, Y. and Kurashige, S.  Establishment of a Tumor-Associated Macrophage Cell Line (TtT/M-87) Form Mouse Thyrotropic Pituitary Tumor  Biomedical Research  1988  9:367-373    DOI: 10.2220/biomedres.9.367

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