Cell No. : Cell Name
RCB0531 : TtT/M-87
update : 2022/03/17
|
| Comment | Mouse cell line derived from pituitary tumor (thyrotropin-producing tumor). Macrophage-like cells. Dependent on conditioned medium of L929 cells. Conditioned medium. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Inoue, Kinji
|
| Originator |
Inoue, Kinji
|
| Year of deposit |
1990
|
| Animal |
_mouse
< Mammals
|
| Gender |
Unknown
|
| Tissue |
pituitary
|
| Disease name |
thyrotropic pituitary tumor
|
| Classification |
cancer
|
| Year of origin |
1985
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_U804
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 15% FBS + 20% (RCB1451 L929 conditioned medium)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
4577
Mitsuishi T, Akuzawa Y, Sato S, Rui J, Kodama K, Inoue K, Kurashige S.
Immunological properties of TtT/M-87 cell line established from murine pituitary tumor-associated macrophages.
Microbiol Immunol
1993
37(12):943-51
PubMed ID: 8133800
DOI: 10.1111/j.1348-0421.1993.tb01728.x
|
|
1290
Inoue, K., Ito, A., Kubota, Y. and Kurashige, S.
Establishment of a Tumor-Associated Macrophage Cell Line (TtT/M-87) Form Mouse Thyrotropic Pituitary Tumor
Biomedical Research
1988
9:367-373
DOI: 10.2220/biomedres.9.367
|
Back
Top
CELL BANK Website