Cell No. : Cell Name RCB0530 : MtT/SM  update : 2024/01/10
Comment			Rat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing prolactin and growth hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously. Application consideration.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Inoue, Kinji
		Originator	Inoue, Kinji
		Year of deposit	1990
		Animal	_rat < Mammals
		Strain name	Fischer
		Gender	Female
		Tissue	pituitary
		Disease name	mammotropic pituitary tumor
		Classification	cancer
		Year of origin	1987
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_H715
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent/Suspension cells
		Culture medium		DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Animal PCR		OK
		Isozyme		LD, NP
		Others		This cells were mainly spherical and formed floating or weakly adherent clusters.
Reference information		Reference	5
		User's Publication	1

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Reference

3323  Kotaro Yokoyama, Mahoko Hayashi, Chihiro Mogi, Yoshihiro Sasakawa, Gen Watanabe, Kazuyoshi Taya, Sukumar Devnath, Kinji Inoue  Dose-dependent effects of a glucocorticoid on prolactin production  Endocr J  2008  55(2):405-14  PubMed ID: 18379123   DOI: 10.1507/endocrj.k07e-063

3322  M Tomida, U Yoshida, C Mogi, M Maruyama, H Goda, Y Hatta, K Inoue  Leukaemia inhibitory factor and interleukin 6 inhibit secretion of prolactin and growth hormone by rat pituitary MtT/SM cells  Cytokine  2001  14(4):202-7  PubMed ID: 11448119   DOI: 10.1006/cyto.2001.0874

32  Kurotani R, Tahara S, Sanno N, Teramoto A, Mellon PL, Inoue K, Yoshimura S, Osamura RY.  Expression of Ptx1 in the adult rat pituitary glands and pituitary cell lines: hormone-secreting cells and folliculo-stellate cells  Cell Tissue Res  1999  298(1):55-61  PubMed ID: 10555539   DOI: 10.1007/pl00008809

3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767   DOI: 10.1111/j.1349-7006.1991.tb01817.x

1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014   DOI: 10.1210/endo-126-5-2313

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User's Publication

9617  K Yoshimoto, C Tanaka, M Moritani, E Shimizu, T Yamaoka, S Yamada, T Sano, M Itakura  Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-Derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas  ndocr J   1999  46(1):199-207  PubMed ID: 10426588   DOI: 10.1507/endocrj.46.199