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Cell No. : Cell Name
RCB0530 : MtT/SM  update : 2021/02/26
CommentRat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing prolactin and growth hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously. Application consideration.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1990
Animal _rat < Mammals
Strain name Fischer
Gender Female
Tissue pituitary
Disease name mammotropic pituitary tumor
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Animal PCR OK
Isozyme LD, NP
Others This cells were mainly spherical and formed floating or weakly adherent clusters.
Reference information Reference 4
User's Publication 1


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Reference
3323  Kotaro Yokoyama, Mahoko Hayashi, Chihiro Mogi, Yoshihiro Sasakawa, Gen Watanabe, Kazuyoshi Taya, Sukumar Devnath, Kinji Inoue  Dose-dependent effects of a glucocorticoid on prolactin production  Endocr J  2008  55(2):405-14  PubMed ID: 18379123
3322  M Tomida, U Yoshida, C Mogi, M Maruyama, H Goda, Y Hatta, K Inoue  Leukaemia inhibitory factor and interleukin 6 inhibit secretion of prolactin and growth hormone by rat pituitary MtT/SM cells  Cytokine  2001  14(4):202-7  PubMed ID: 11448119
3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767
1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014

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User's Publication
9617  K Yoshimoto, C Tanaka, M Moritani, E Shimizu, T Yamaoka, S Yamada, T Sano, M Itakura  Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-Derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas  ndocr J   1999  46(1):199-207  PubMed ID: 10426588



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