Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0529 : MtT/Se  update : 2022/03/17
CommentRat cell line derived from pituitary tumor. Estrogen-induced tumor. Estrogen-dependent cell line. Producing a small amount of growth hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1990
Animal rat < Mammals
Strain name Fischer
Gender Female
Tissue pituitary
Disease name mammotropic pituitary tumor
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_H714
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO) + 10^(-9)M Estrogen
Antibiotics Free
Passage method 0.25% Trypsin or pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 3


To topTop
Reference
3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767   DOI: 10.1111/j.1349-7006.1991.tb01817.x
1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014  

To topTop
User's Publication
10797  Wang Z, Kanda S, Shimono T, Enkh-Undraa D, Nishiyama T.  The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them.  BMC Complement Altern Med  2018  18:107  PubMed ID: 29566679   DOI: 10.1186/s12906-018-2170-4
12576  Mari Manabe, Seiji Kanda, Kenji Fukunaga, Airo Tsubura, Toshimasa Nishiyama  Evaluation of the estrogenic activities of some pesticides and their combinations using MtT/Se cell proliferation assay  Int J Hyg Environ Health  2006  ;209(5):413-21  PubMed ID: 16781191   DOI: 10.1016/j.ijheh.2006.04.004
12581  H Takahashi, S Nakagawa  Effects of estrogen on cell growth and fibroblast growth factor receptor induction in MtT/Se cells  Endocr Res  1997  23(1-2):95-104  PubMed ID: 918754   DOI: 10.1080/07435809709031845



Back Back Return Top Page