Cell No. : Cell Name RCB0529 : MtT/Se  update : 2022/03/17
Comment			Rat cell line derived from pituitary tumor. Estrogen-induced tumor. Estrogen-dependent cell line. Producing a small amount of growth hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Inoue, Kinji
		Originator	Inoue, Kinji
		Year of deposit	1990
		Animal	_rat < Mammals
		Strain name	Fischer
		Gender	Female
		Tissue	pituitary
		Disease name	mammotropic pituitary tumor
		Classification	cancer
		Year of origin	1987
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_H714
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO) + 10 -9M Estrogen
		Antibiotics		Free
		Passage method		0.25% Trypsin or pipetting
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	2
		User's Publication	3

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Reference

3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767   DOI: 10.1111/j.1349-7006.1991.tb01817.x

1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014   DOI: 10.1210/endo-126-5-2313

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User's Publication

10797  Wang Z, Kanda S, Shimono T, Enkh-Undraa D, Nishiyama T.  The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them.  BMC Complement Altern Med  2018  18:107  PubMed ID: 29566679   DOI: 10.1186/s12906-018-2170-4

12576  Mari Manabe, Seiji Kanda, Kenji Fukunaga, Airo Tsubura, Toshimasa Nishiyama  Evaluation of the estrogenic activities of some pesticides and their combinations using MtT/Se cell proliferation assay  Int J Hyg Environ Health  2006  ;209(5):413-21  PubMed ID: 16781191   DOI: 10.1016/j.ijheh.2006.04.004

12581  H Takahashi, S Nakagawa  Effects of estrogen on cell growth and fibroblast growth factor receptor induction in MtT/Se cells  Endocr Res  1997  23(1-2):95-104  PubMed ID: 9187541   DOI: 10.1080/07435809709031845