細胞番号 : 細胞名
RCB0528 : MtT/S
update : 2022/11/18
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| 細胞特性(Comment:英) | Rat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing growth hormone. Able to induce the cells producing prolactin. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration. |
| 細胞特性(日) | ラット下垂体腫瘍由来細胞株。エストロジェン誘導性腫瘍。Growth hormoneを分泌する。プロラクチン産生細胞を分化誘導することができる。MtT/E, MtT/S, MtT/SM, MtT/Seの４株は同時に樹立された細胞株。培養開始にあたっての注意。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Inoue, Kinji
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| 樹立者 |
Inoue, Kinji
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| 寄託日 |
1990
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| 動物種 |
_rat
< Mammals
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| 動物系統名 |
Fischer
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| 性別 |
Female
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| 採取組織 |
pituitary
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| 病名 |
mammotropic pituitary tumor
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| 細胞分類 |
cancer
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| 初代培養日 |
1987
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| メモ_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
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細胞寿命 |
infinite
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| 細胞形態 |
other
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| Cellosaurus(Expasy) |
CVCL_H713
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent/Suspension cells
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| 培地 |
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DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture : once/2 weeks, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| 動物種PCR |
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OK
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| アイソザイム検査 |
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LD, NP
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
19件
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| Reference(日) |
1件
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| 利用者成果(英) |
9件
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| 利用者成果(日) |
0件
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| Reference(英) |
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3423
Risa Kawashima, Kazuya Ikematsu, Yuki Abe, Mai Sato, Shinichiro Tsuruya, Ichiro Nakasono, Hiroyuki Fukushima, Kinji Inoue, Takashi Tsuboi
Effect of glucocorticoid on the biosynthesis of growth hormone-containing secretory granules in pituitary cells
Biochem Biophys Res Commun
2010
400(2):225-9
PubMed ID: 20723537
DOI: 10.1016/j.bbrc.2010.08.044
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3424
Sukumar Devnath, Taro Kataoka, Kaoru Miura, Mie Kusuda, Kunio Kitamura, Yuko Kumada, Akikazu Mochiduki, Kei Kaneko, Akihito Adachi, Kinji Inoue
Cgr11 encodes a secretory protein involved in cell adhesion
Eur J Cell Biol
2009
88(9):521-9
PubMed ID: 19473726
DOI: 10.1016/j.ejcb.2009.04.003
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3422
Kotaro Yokoyama, Chihiro Mogi, Kaoru Miura, Keisuke Kuroda, Kinji Inoue
Somatotropes maintain their immature cells through Insulin-like growth factor I (IGF-I)
Endocr Pathol . Fall 2007;18(3):174-81
2007
18(3):174-81
PubMed ID: 18058266
DOI: 10.1007/s12022-007-0016-6
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3426
Haruo Nogami, Yoshiki Hiraoka, Kinji Inoue, Sadakazu Aiso, Setsuji Hisano
Regulation of 5'-promoter activity of the rat growth hormone and growth hormone-releasing hormone receptor genes in the MtT/S and MtT/E cells
Neuroendocrinology
2006
84(1):31-41
PubMed ID: 17090972
DOI: 10.1159/000096826
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3425
C Mogi, H Goda, K Mogi, A Takaki, K Yokoyama, M Tomida, K Inoue
Multistep differentiation of GH-producing cells from their immature cells
J Endocrinol
2005
184(1):41-50
PubMed ID: 15642781
DOI: 10.1677/joe.1.05870
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3421
Yasumasa Iwasaki, Minako Morishita, Masato Asai, Akiko Onishi, Masanori Yoshida, Yutaka Oiso, Kinji Inoue
Effects of hormones targeting nuclear receptors on transcriptional regulation of the growth hormone gene in the MtT/S rat somatotrope cell line
Neuroendocrinology
2004
79(5):229-36
PubMed ID: 15240998
DOI: 10.1159/000078787
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3315
M Morishita, Y Iwasaki, A Onishi, M Asai, N Mutsuga, M Yoshida, Y Oiso, K Inoue, T Murohara
The effects of GH-releasing hormone/somatostatin on the 5'-promoter activity of the GH gene in vitro
J Mol Endocrinol
2003
31(3):441-8
PubMed ID: 14664705
DOI: 10.1677/jme.0.0310441
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3406
A Niiori-Onishi, Y Iwasaki, N Mutsuga, Y Oiso, K Inoue, H Saito
Molecular mechanisms of the negative effect of insulin-like growth factor-I on growth hormone gene expression in MtT/S somatotroph cells
Endocrinology
1999
140(1):344-9
PubMed ID: 9886844
DOI: 10.1210/endo.140.1.6405
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976
Nogami H, Inoue K, Moriya H, Ishida A, Kobayashi S, Hisano S, Katayama M, Kawamura K.
Regulation of growth hormone-releasing hormone receptor messenger ribonucleic acid expression by glucocorticoids in MtT-S cells and in the pituitary gland of fetal rats
Endocrinology
1999
140(6):2763-70
PubMed ID: 10342867
DOI: 10.1210/endo.140.6.6787
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960
Inoue K, Couch EF, Takano K, Ogawa S.
The structure and function of folliculo-stellate cells in the anterior pituitary gland
Arch Histol Cytol
1999
62(3):205-18
PubMed ID: 10495875
DOI: 10.1679/aohc.62.205
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3394
H Goda, T Sakai, M Kurosumi, K Inoue
Prolactin-producing cells differentiate from G0/G1-arrested somatotrophs in vitro: an analysis of cell cycle phases and mammotroph differentiation
Endocr J
1998
45(6):725-35
PubMed ID: 10395227
DOI: 10.1507/endocrj.45.725
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3420
Chiaki Koyama, Hirokazu Matsumoto, Takafumi Sakai, Katsumi Wakabayashi, Akihiro Ito, Ernest F. Couch, Kinji Inoue
Pituitary Folliculo-Stellate-Like Cells Stimulate Somatotroic Pituitary Tumor Growth in Nude Mice
Endocr Pathol
1995
6(1):67-75
PubMed ID: 12114691
DOI: 10.1007/BF02914990
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3316
S Nagata, T Uehara, K Inoue, T Senshu
Increased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line, MtT/S
J Cell Physiol
1992
150(2):426-32
PubMed ID: 1734040
DOI: 10.1002/jcp.1041500227
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3401
N Fujimoto, H Watanabe, A Ito, K Inoue
Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats
Jpn J Cancer Res
1991
82(12):1436-41
PubMed ID: 1778767
DOI: 10.1111/j.1349-7006.1991.tb01817.x
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3317
K Inoue, T Sakai
Conversion of growth hormone-secreting cells into prolactin-secreting cells and its promotion by insulin and insulin-like growth factor-1 in vitro
Exp Cell Res
1991
195(1):53-8
PubMed ID: 2055276
DOI: 10.1016/0014-4827(91)90499-k
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3319
S Nagata, K Inoue, T Senshu
Estrogen-insulin synergism in induction of prolactin in growth hormone-producing cells
Mol Cell Endocrinol
1991
82(2-3):215-20
PubMed ID: 1794611
DOI: 10.1016/0303-7207(91)90034-p
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3321
K Inoue, T Sakai, M Hattori
The cell-adhesive effect of basic fibroblast growth factor on pituitary cells in vitro
J Endocrinol
1991
130(3):381-6
PubMed ID: 1940714
DOI: 10.1677/joe.0.1300381
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1289
Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A
Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.
Endocrinology
1990
126:2313-20
PubMed ID: 2184014
DOI: 10.1210/endo-126-5-2313
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3318
S Nagata, M Yamagiwa, K Inoue, T Senshu
Estrogen regulates peptidylarginine deiminase levels in a rat pituitary cell line in culture
J Cell Physiol
1990
145(2):333-9
PubMed ID: 2246332
DOI: 10.1002/jcp.1041450219
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| 利用者成果(英) |
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12573
Dimiter Avtanski, Horacio J Novaira, Sheng Wu, Christopher J Romero, Rhonda Kineman, Raul M Luque, Fredric Wondisford, Sally Radovick
Both estrogen receptor α and β stimulate pituitary GH gene expression
Mol Endocrinol
2014
28(1):40-52
PubMed ID: 24284820
DOI: 10.1210/me.2013-1245
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12570
Chikari Takeo, Kazuhiro Ikeda, Kuniko Horie-Inoue, Satoshi Inoue
Identification of Igf2, Igfbp2 and Enpp2 as estrogen-responsive genes in rat hippocampus
Endocr J
2009
56(1):113-20
PubMed ID: 18946176
DOI: 10.1507/endocrj.k08e-220
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12567
Feng-Ying Gong, Jie-Ying Deng, Yi-Fan Shi
Mek and p38 MAPK-dependant pathways are involoved in the positive effect of interleukin-6 on human growth hormone gene expression in rat MtT/S somatotroph cells
Chin Med Sci J
2008
23(2):73-80
PubMed ID: 18686625
DOI: 10.1016/s1001-9294(09)60016-x
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12568
Feng-ying Gong, Jie-ying Deng, Yi-fan Shi
Effect of interleukin-1 beta on growth hormone gene expression and its possible molecular mechanism in rat MtT/S somatotroph cells
Chin Med Sci J
2008
23(4):193-201
PubMed ID: 19180878
DOI: 10.1016/s1001-9294(09)60038-9
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12566
T C Voss, L R Goldman, S L Seek, T L Miller, K E Mayo, A Somogyvari-Vigh, A Arimura, D L Hurley
GH mRNA levels are elevated by forskolin but not GH releasing hormone in GHRH receptor-expressing MtT/S somatotroph cell line
Mol Cell Endocrinol
2001
172(1-2):125-34
PubMed ID: 11165046
DOI: 10.1016/s0303-7207(00)00376-2
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18652
Ohkawa K, Takada K, Asakura T, Hashizume Y, Okawa Y, Tashiro K, Ueda J, Itoh Y, Hibi N.
Calpain inhibitor inhibits secretory granule maturation and secretion of GH
Neuroreport
2000
11(18):4007-11
PubMed ID: 11192619
DOI: 10.1097/00001756-200012180-00021
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12569
T C Voss, T M Mangin, D L Hurley
Insulin-like growth factor-1 causes a switch-like reduction of endogenous growth hormone mRNA in rat MtT/S somatotroph cells
Endocrine
2000
13(1):71-9
PubMed ID: 11051049
DOI: 10.1385/ENDO:13:1:71
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9617
K Yoshimoto, C Tanaka, M Moritani, E Shimizu, T Yamaoka, S Yamada, T Sano, M Itakura
Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-Derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas
ndocr J
1999
46(1):199-207
PubMed ID: 10426588
DOI: 10.1507/endocrj.46.199
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12571
Y Hayashi, M Yamamoto, S Ohmori, T Kamijo, M Ogawa, H Seo
Inhibition of growth hormone (GH) secretion by a mutant GH-I gene product in neuroendocrine cells containing secretory granules: an implication for isolated GH deficiency inherited in an autosomal dominant manner
J Clin Endocrinol Metab
1999
84(6):2134-9
PubMed ID: 10372722
DOI: 10.1210/jcem.84.6.5736
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