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Cell No. : Cell Name
RCB0528 : MtT/S  update : 2022/11/18
CommentRat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing growth hormone. Able to induce the cells producing prolactin. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1990
Animal rat < Mammals
Strain name Fischer
Gender Female
Tissue pituitary
Disease name mammotropic pituitary tumor
Classification cancer
Year of origin 1987
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_H713
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 17
User's Publication 8

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3423  Risa Kawashima, Kazuya Ikematsu, Yuki Abe, Mai Sato, Shinichiro Tsuruya, Ichiro Nakasono, Hiroyuki Fukushima, Kinji Inoue, Takashi Tsuboi  Effect of glucocorticoid on the biosynthesis of growth hormone-containing secretory granules in pituitary cells  Biochem Biophys Res Commun  2010  400(2):225-9  PubMed ID: 20723537
3424  Sukumar Devnath, Taro Kataoka, Kaoru Miura, Mie Kusuda, Kunio Kitamura, Yuko Kumada, Akikazu Mochiduki, Kei Kaneko, Akihito Adachi, Kinji Inoue  Cgr11 encodes a secretory protein involved in cell adhesion  Eur J Cell Biol  2009  88(9):521-9  PubMed ID: 19473726
3422  Kotaro Yokoyama, Chihiro Mogi, Kaoru Miura, Keisuke Kuroda, Kinji Inoue  Somatotropes maintain their immature cells through Insulin-like growth factor I (IGF-I)  Endocr Pathol . Fall 2007;18(3):174-81  2007  18(3):174-81  PubMed ID: 18058266
3426  Haruo Nogami, Yoshiki Hiraoka, Kinji Inoue, Sadakazu Aiso, Setsuji Hisano  Regulation of 5'-promoter activity of the rat growth hormone and growth hormone-releasing hormone receptor genes in the MtT/S and MtT/E cells  Neuroendocrinology  2006  84(1):31-41  PubMed ID: 17090972
3425  C Mogi, H Goda, K Mogi, A Takaki, K Yokoyama, M Tomida, K Inoue  Multistep differentiation of GH-producing cells from their immature cells  J Endocrinol   2005  184(1):41-50  PubMed ID: 15642781
3421  Yasumasa Iwasaki, Minako Morishita, Masato Asai, Akiko Onishi, Masanori Yoshida, Yutaka Oiso, Kinji Inoue  Effects of hormones targeting nuclear receptors on transcriptional regulation of the growth hormone gene in the MtT/S rat somatotrope cell line  Neuroendocrinology  2004  79(5):229-36  PubMed ID: 15240998
3315  M Morishita, Y Iwasaki, A Onishi, M Asai, N Mutsuga, M Yoshida, Y Oiso, K Inoue, T Murohara  The effects of GH-releasing hormone/somatostatin on the 5'-promoter activity of the GH gene in vitro  J Mol Endocrinol  2003  31(3):441-8  PubMed ID: 14664705
3406  A Niiori-Onishi, Y Iwasaki, N Mutsuga, Y Oiso, K Inoue, H Saito  Molecular mechanisms of the negative effect of insulin-like growth factor-I on growth hormone gene expression in MtT/S somatotroph cells  Endocrinology  1999  140(1):344-9  PubMed ID: 9886844
3394  H Goda, T Sakai, M Kurosumi, K Inoue  Prolactin-producing cells differentiate from G0/G1-arrested somatotrophs in vitro: an analysis of cell cycle phases and mammotroph differentiation  Endocr J  1998  45(6):725-35  PubMed ID: 10395227
3420  Chiaki Koyama, Hirokazu Matsumoto, Takafumi Sakai, Katsumi Wakabayashi, Akihiro Ito, Ernest F. Couch, Kinji Inoue  Pituitary Folliculo-Stellate-Like Cells Stimulate Somatotroic Pituitary Tumor Growth in Nude Mice  Endocr Pathol  1995  6(1):67-75  PubMed ID: 12114691
3316  S Nagata, T Uehara, K Inoue, T Senshu  Increased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line, MtT/S  J Cell Physiol   1992  150(2):426-32  PubMed ID: 1734040
3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767
3317  K Inoue, T Sakai  Conversion of growth hormone-secreting cells into prolactin-secreting cells and its promotion by insulin and insulin-like growth factor-1 in vitro  Exp Cell Res  1991  195(1):53-8  PubMed ID: 2055276
3319  S Nagata, K Inoue, T Senshu  Estrogen-insulin synergism in induction of prolactin in growth hormone-producing cells  Mol Cell Endocrinol  1991  82(2-3):215-20  PubMed ID: 1794611
3321  K Inoue, T Sakai, M Hattori  The cell-adhesive effect of basic fibroblast growth factor on pituitary cells in vitro  J Endocrinol  1991  130(3):381-6  PubMed ID: 1940714
1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014
3318  S Nagata, M Yamagiwa, K Inoue, T Senshu  Estrogen regulates peptidylarginine deiminase levels in a rat pituitary cell line in culture  J Cell Physiol  1990  145(2):333-9  PubMed ID: 2246332

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User's Publication
12573  Dimiter Avtanski, Horacio J Novaira, Sheng Wu, Christopher J Romero, Rhonda Kineman, Raul M Luque, Fredric Wondisford, Sally Radovick  Both estrogen receptor α and β stimulate pituitary GH gene expression  Mol Endocrinol  2014  28(1):40-52  PubMed ID: 24284820
12570  Chikari Takeo, Kazuhiro Ikeda, Kuniko Horie-Inoue, Satoshi Inoue  Identification of Igf2, Igfbp2 and Enpp2 as estrogen-responsive genes in rat hippocampus  Endocr J  2009  56(1):113-20  PubMed ID: 18946176
12567  Feng-Ying Gong, Jie-Ying Deng, Yi-Fan Shi  Mek and p38 MAPK-dependant pathways are involoved in the positive effect of interleukin-6 on human growth hormone gene expression in rat MtT/S somatotroph cells  Chin Med Sci J   2008  23(2):73-80  PubMed ID: 18686625
12568  Feng-ying Gong, Jie-ying Deng, Yi-fan Shi  Effect of interleukin-1 beta on growth hormone gene expression and its possible molecular mechanism in rat MtT/S somatotroph cells  Chin Med Sci J  2008  23(4):193-201  PubMed ID: 19180878
12566  T C Voss, L R Goldman, S L Seek, T L Miller, K E Mayo, A Somogyvari-Vigh, A Arimura, D L Hurley  GH mRNA levels are elevated by forskolin but not GH releasing hormone in GHRH receptor-expressing MtT/S somatotroph cell line  Mol Cell Endocrinol  2001  172(1-2):125-34  PubMed ID: 11165046
12569  T C Voss, T M Mangin, D L Hurley  Insulin-like growth factor-1 causes a switch-like reduction of endogenous growth hormone mRNA in rat MtT/S somatotroph cells  Endocrine  2000  13(1):71-9  PubMed ID: 11051049
9617  K Yoshimoto, C Tanaka, M Moritani, E Shimizu, T Yamaoka, S Yamada, T Sano, M Itakura  Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-Derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas  ndocr J   1999  46(1):199-207  PubMed ID: 10426588
12571  Y Hayashi, M Yamamoto, S Ohmori, T Kamijo, M Ogawa, H Seo  Inhibition of growth hormone (GH) secretion by a mutant GH-I gene product in neuroendocrine cells containing secretory granules: an implication for isolated GH deficiency inherited in an autosomal dominant manner  J Clin Endocrinol Metab  1999  84(6):2134-9  PubMed ID: 10372722

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