細胞番号 : 細胞名
RCB0527 : Jyg-MC(B)
update : 2022/03/17
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| 細胞特性(Comment:英) | Murine mammary tumor cell lines. Higher incidence in lung metastasis. |
| 細胞特性(日) | マウス乳癌。Jyg-MC(A)と同様だが，Jyg-MC(A)よりも肺転移の頻度が高い。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Morimoto, Junji
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| 樹立者 |
Morimoto, Junji
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| 寄託日 |
1990
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
JYG
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| 性別 |
Female
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| 採取組織 |
mammary
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| 病名 |
mammary tumor
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| 転移 |
Yes
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| 転移巣 |
lung,liver, spleen
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| 細胞分類 |
cancer
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| 初代培養日 |
1987
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|
細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_H217
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM (low glucose) + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| マウス系統検査 |
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OK
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| アイソザイム検査 |
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LD, NP, G6PD, AST, MPI
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| 染色体検査 |
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38-160(50) : /39(5),40(25),41(3),75(1),76(1),77(1)/
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
9件
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| Reference(日) |
0件
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| 利用者成果(英) |
6件
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| 利用者成果(日) |
0件
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| Reference(英) |
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979
Shibata MA, Ambati J, Shibata E, Albuquerque RJ, Morimoto J, Ito Y, Otsuki Y.
The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model
BMC Med
2010
8:69
PubMed ID: 21047425
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986
Shibata MA, Shibata E, Morimoto J, Eid NA, Tanaka Y, Watanabe M, Otsuki Y.
An immunocompetent murine model of metastatic mammary cancer accessible to bioluminescence imaging
Anticancer Res
2009
29(11):4389-95
PubMed ID: 20032383
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978
Shibata MA, Morimoto J, Shibata E, Otsuki Y.
Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model
Cancer Gene Ther
2008
15(12):776-86
PubMed ID: 18654613
DOI: 10.1038/cgt.2008.43
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974
Shibata MA, Morimoto J, Akamatsu K, Otsuki Y.
Antimetastatic effect of suicide gene therapy for mouse mammary cancers requires T-cell-mediated immune responses
Med Mol Morphol
2008
41(1):34-43
PubMed ID: 18470679
DOI: 10.1007/s00795-007-0388-1
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975
Shibata MA, Morimoto J, Doi H, Morishima S, Naka M, Otsuki Y.
Electrogene therapy using endostatin, with or without suicide gene therapy, suppresses murine mammary tumor growth and metastasis
Cancer Gene Ther
2007
14(3):268-78
PubMed ID: 17096028
DOI: 10.1038/sj.cgt.7701009
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1004
Shibata MA, Morimoto J, Ito Y, Kusakabe K, Otsuki Y.
Experimental gene therapy in mammary and urinary bladder cancer using electrogene transfer
Med Electron Microsc
2004
37(4):216-24
PubMed ID: 15614446
DOI: 10.1007/s00795-004-0247-2
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1001
Shibata MA, Morimoto J, Otsuki Y.
Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation
Cancer Gene Ther
2002
9(1):16-27
PubMed ID: 11916241
DOI: 10.1038/sj.cgt.7700415
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1432
Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L
New murine mammary tumor cell lines.
In Vitro Cell Dev Biol
1991
27A:349-51
PubMed ID: 2071538
DOI: 10.1007/BF02630952
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1003
Kiyozuka Y, Asai A, Senzaki H, Uemura Y, Nakashima A, Morimoto J, Matsuzawa A, Tsubura A.
Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression
Int J Mol Med
1988
2(4):437-44
PubMed ID: 9857231
DOI: 10.3892/ijmm.2.4.437
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| 利用者成果(英) |
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14659
Okita Y, Kimura M, Xie R, Chen C, Shen LT, Kojima Y, Suzuki H, Muratani M, Saitoh M, Semba K, Heldin CH, Kato M.
The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB
Sci Signal
2017
10(474):eaak9397
PubMed ID: 28400538
DOI: 10.1126/scisignal.aak9397
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8167
Takekoshi T, Tada Y, Watanabe T, Sugaya M, Hoashi T, Komine M, Kawashima T, Shimizu T, Hau CS, Asahina A, Yokomizo T, Sato S, Tamaki K.
Identification of a novel marker for dendritic cell maturation, mouse transmembrane protein 123.
J. Biol. Chem.
2010
285:31876-84
PubMed ID: 20685650
DOI: 10.1074/jbc.M110.118877
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6302
Cornils K, Lange C, Schambach A, Brugman MH, Nowak R, Lioznov M, Baum C, Fehse B.
Stem cell marking with promotor-deprived self-inactivating retroviral vectors does not lead to induced clonal imbalance.
Mol Ther
2009
17(1):131-43
PubMed ID: 19002163
DOI: 10.1038/mt.2008.238
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16430
Montani L, Gerrits B, Gehrig P, Kempf A, Dimou L, Wollscheid B, Schwab ME.
Neuronal Nogo-A modulates growth cone motility via Rho-GTP/LIMK1/cofilin in the unlesioned adult nervous system
J Biol Chem
2009
284(16):10793-807
PubMed ID: 19208621
DOI: 10.1074/jbc.M808297200
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8849
Beisel KW, Rocha-Sanchez SM, Ziegenbein SJ, Morris KA, Kai C, Kawai J, Carninci P, Hayashizaki Y, Davis RL.
Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells.
Gene
2007
386:11-23
PubMed ID: 17097837
DOI: 10.1016/j.gene.2006.07.023
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9058
Galliano MF, Roccasecca RM, Descargues P, Micheloni A, Levy E, Zambruno G, D'Alessio M, Hovnanian A.
Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome.
Genomics
2005
85:483-92
PubMed ID: 15780751
DOI: 10.1016/j.ygeno.2005.01.001
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