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Cell No. : Cell Name
RCB0527 : Jyg-MC(B)  update : 2022/03/17
CommentMurine mammary tumor cell lines. Higher incidence in lung metastasis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Morimoto, Junji
Originator Morimoto, Junji
Year of deposit 1990
Animal _mouse < Mammals
Strain name JYG
Gender Female
Tissue mammary
Disease name mammary tumor
Metastatic ability Yes
Metastatic tissue lung,liver, spleen
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H217
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 38-160(50) : /39(5),40(25),41(3),75(1),76(1),77(1)/
Images
deposit info
lot info
Reference information Reference 9
User's Publication 6


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Reference
979  Shibata MA, Ambati J, Shibata E, Albuquerque RJ, Morimoto J, Ito Y, Otsuki Y.  The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model  BMC Med  2010  8:69  PubMed ID: 21047425  
986  Shibata MA, Shibata E, Morimoto J, Eid NA, Tanaka Y, Watanabe M, Otsuki Y.  An immunocompetent murine model of metastatic mammary cancer accessible to bioluminescence imaging  Anticancer Res  2009  29(11):4389-95  PubMed ID: 20032383  
978  Shibata MA, Morimoto J, Shibata E, Otsuki Y.  Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model  Cancer Gene Ther  2008  15(12):776-86  PubMed ID: 18654613   DOI: 10.1038/cgt.2008.43
974  Shibata MA, Morimoto J, Akamatsu K, Otsuki Y.  Antimetastatic effect of suicide gene therapy for mouse mammary cancers requires T-cell-mediated immune responses  Med Mol Morphol  2008  41(1):34-43  PubMed ID: 18470679   DOI: 10.1007/s00795-007-0388-1
975  Shibata MA, Morimoto J, Doi H, Morishima S, Naka M, Otsuki Y.  Electrogene therapy using endostatin, with or without suicide gene therapy, suppresses murine mammary tumor growth and metastasis  Cancer Gene Ther  2007  14(3):268-78  PubMed ID: 17096028   DOI: 10.1038/sj.cgt.7701009
1004  Shibata MA, Morimoto J, Ito Y, Kusakabe K, Otsuki Y.  Experimental gene therapy in mammary and urinary bladder cancer using electrogene transfer  Med Electron Microsc  2004  37(4):216-24  PubMed ID: 15614446   DOI: 10.1007/s00795-004-0247-2
1001  Shibata MA, Morimoto J, Otsuki Y.  Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation  Cancer Gene Ther  2002  9(1):16-27  PubMed ID: 11916241   DOI: 10.1038/sj.cgt.7700415
1432  Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L  New murine mammary tumor cell lines.  In Vitro Cell Dev Biol  1991  27A:349-51  PubMed ID: 2071538   DOI: 10.1007/BF02630952
1003  Kiyozuka Y, Asai A, Senzaki H, Uemura Y, Nakashima A, Morimoto J, Matsuzawa A, Tsubura A.  Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression  Int J Mol Med  1988  2(4):437-44  PubMed ID: 9857231   DOI: 10.3892/ijmm.2.4.437

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User's Publication
14659  Okita Y, Kimura M, Xie R, Chen C, Shen LT, Kojima Y, Suzuki H, Muratani M, Saitoh M, Semba K, Heldin CH, Kato M.  The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB  Sci Signal  2017  10(474):eaak9397  PubMed ID: 28400538   DOI: 10.1126/scisignal.aak9397
8167  Takekoshi T, Tada Y, Watanabe T, Sugaya M, Hoashi T, Komine M, Kawashima T, Shimizu T, Hau CS, Asahina A, Yokomizo T, Sato S, Tamaki K.  Identification of a novel marker for dendritic cell maturation, mouse transmembrane protein 123.  J. Biol. Chem.  2010  285:31876-84  PubMed ID: 20685650   DOI: 10.1074/jbc.M110.118877
6302  Cornils K, Lange C, Schambach A, Brugman MH, Nowak R, Lioznov M, Baum C, Fehse B.  Stem cell marking with promotor-deprived self-inactivating retroviral vectors does not lead to induced clonal imbalance.  Mol Ther  2009  17(1):131-43  PubMed ID: 19002163   DOI: 10.1038/mt.2008.238
16430  Montani L, Gerrits B, Gehrig P, Kempf A, Dimou L, Wollscheid B, Schwab ME.  Neuronal Nogo-A modulates growth cone motility via Rho-GTP/LIMK1/cofilin in the unlesioned adult nervous system  J Biol Chem  2009  284(16):10793-807  PubMed ID: 19208621   DOI: 10.1074/jbc.M808297200
8849  Beisel KW, Rocha-Sanchez SM, Ziegenbein SJ, Morris KA, Kai C, Kawai J, Carninci P, Hayashizaki Y, Davis RL.  Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells.  Gene  2007  386:11-23  PubMed ID: 17097837   DOI: 10.1016/j.gene.2006.07.023
9058  Galliano MF, Roccasecca RM, Descargues P, Micheloni A, Levy E, Zambruno G, D'Alessio M, Hovnanian A.  Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome.  Genomics  2005  85:483-92  PubMed ID: 15780751   DOI: 10.1016/j.ygeno.2005.01.001


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