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Cell No. : Cell Name
RCB0527 : Jyg-MC(B)  update : 2018/07/06
CommentMurine mammary tumor cell lines. .Higher incidence in lung metastasis.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Morimoto, Junji
Originator Morimoto, Junji
Year of deposit 1990
Animal mouse < Mammals
Strain name JYG
Gender Female
Tissue mammary
Case history mammary tumor
Metastastatic ability Yes
Metastatsd tissue lung,liver, spleen
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 38-160(50) : /39(5),40(25),41(3),75(1),76(1),77(1)/
deposit info
lot info
Reference information Reference 1
User's Publication 4

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1432  Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L  New murine mammary tumor cell lines.  In Vitro Cell Dev Biol  1991  27A:349-51  PubMed ID: 2071538

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User's Publication
8167  Takekoshi T, Tada Y, Watanabe T, Sugaya M, Hoashi T, Komine M, Kawashima T, Shimizu T, Hau CS, Asahina A, Yokomizo T, Sato S, Tamaki K.  Identification of a novel marker for dendritic cell maturation, mouse transmembrane protein 123.  J. Biol. Chem.  2010  285:31876-84  PubMed ID: 20685650
6302  Cornils K, Lange C, Schambach A, Brugman MH, Nowak R, Lioznov M, Baum C, Fehse B.  Stem cell marking with promotor-deprived self-inactivating retroviral vectors does not lead to induced clonal imbalance.  Mol Ther  2009  17(1):131-43  PubMed ID: 19002163
8849  Beisel KW, Rocha-Sanchez SM, Ziegenbein SJ, Morris KA, Kai C, Kawai J, Carninci P, Hayashizaki Y, Davis RL.  Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells.  Gene  2006  386:11-23  PubMed ID: 17097837
9058  Galliano MF, Roccasecca RM, Descargues P, Micheloni A, Levy E, Zambruno G, D'Alessio M, Hovnanian A.  Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome.  Genomics  2005  85:483-92  PubMed ID: 15780751

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