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Cell No. : Cell Name
RCB0527 : Jyg-MC(B)  update : 2022/03/17
CommentMurine mammary tumor cell lines. Higher incidence in lung metastasis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Morimoto, Junji
Originator Morimoto, Junji
Year of deposit 1990
Animal mouse < Mammals
Strain name JYG
Gender Female
Tissue mammary
Disease name mammary tumor
Metastastatic ability Yes
Metastatsd tissue lung,liver, spleen
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H217
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 38-160(50) : /39(5),40(25),41(3),75(1),76(1),77(1)/
deposit info
lot info
Reference information Reference 1
User's Publication 6

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1432  Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L  New murine mammary tumor cell lines.  In Vitro Cell Dev Biol  1991  27A:349-51  PubMed ID: 2071538  

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User's Publication
14659  Okita Y, Kimura M, Xie R, Chen C, Shen LT, Kojima Y, Suzuki H, Muratani M, Saitoh M, Semba K, Heldin CH, Kato M.  The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB  Sci Signal  2017  10(474):eaak9397  PubMed ID: 28400538   DOI: 10.1126/scisignal.aak9397
8167  Takekoshi T, Tada Y, Watanabe T, Sugaya M, Hoashi T, Komine M, Kawashima T, Shimizu T, Hau CS, Asahina A, Yokomizo T, Sato S, Tamaki K.  Identification of a novel marker for dendritic cell maturation, mouse transmembrane protein 123.  J. Biol. Chem.  2010  285:31876-84  PubMed ID: 20685650   DOI: 10.1074/jbc.M110.118877
6302  Cornils K, Lange C, Schambach A, Brugman MH, Nowak R, Lioznov M, Baum C, Fehse B.  Stem cell marking with promotor-deprived self-inactivating retroviral vectors does not lead to induced clonal imbalance.  Mol Ther  2009  17(1):131-43  PubMed ID: 19002163   DOI: 10.1038/mt.2008.238
16430  Montani L, Gerrits B, Gehrig P, Kempf A, Dimou L, Wollscheid B, Schwab ME.  Neuronal Nogo-A modulates growth cone motility via Rho-GTP/LIMK1/cofilin in the unlesioned adult nervous system  J Biol Chem  2009  284(16):10793-807  PubMed ID: 19208621   DOI: 10.1074/jbc.M808297200
8849  Beisel KW, Rocha-Sanchez SM, Ziegenbein SJ, Morris KA, Kai C, Kawai J, Carninci P, Hayashizaki Y, Davis RL.  Diversity of Ca2+-activated K+ channel transcripts in inner ear hair cells.  Gene  2007  386:11-23  PubMed ID: 17097837   DOI: 10.1016/j.gene.2006.07.023
9058  Galliano MF, Roccasecca RM, Descargues P, Micheloni A, Levy E, Zambruno G, D'Alessio M, Hovnanian A.  Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome.  Genomics  2005  85:483-92  PubMed ID: 15780751   DOI: 10.1016/j.ygeno.2005.01.001

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