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Cell No. : Cell Name
RCB0526 : Jyg-MC(A)  update : 2022/12/21
Commentmurine mammary tumor cell lines. High level MMTV producer line.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Morimoto, Junji
Originator Morimoto, Junji
Year of deposit 1990
Animal _mouse < Mammals
Strain name JYG
Gender Female
Tissue mammary
Disease name mammary tumor
Metastatic ability Yes
Metastatic tissue lung, liver,spleen
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H216
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Chromosome mode 38-126(50) : /39(7),40(11),41(4),55(1),58(2),59(1)/
Images
deposit info
lot info
Reference information Reference 9
User's Publication 8


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Reference
979  Shibata MA, Ambati J, Shibata E, Albuquerque RJ, Morimoto J, Ito Y, Otsuki Y.  The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model  BMC Med  2010  8:69  PubMed ID: 21047425  
986  Shibata MA, Shibata E, Morimoto J, Eid NA, Tanaka Y, Watanabe M, Otsuki Y.  An immunocompetent murine model of metastatic mammary cancer accessible to bioluminescence imaging  Anticancer Res  2009  29(11):4389-95  PubMed ID: 20032383  
978  Shibata MA, Morimoto J, Shibata E, Otsuki Y.  Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model  Cancer Gene Ther  2008  15(12):776-86  PubMed ID: 18654613   DOI: 10.1038/cgt.2008.43
974  Shibata MA, Morimoto J, Akamatsu K, Otsuki Y.  Antimetastatic effect of suicide gene therapy for mouse mammary cancers requires T-cell-mediated immune responses  Med Mol Morphol  2008  41(1):34-43  PubMed ID: 18470679   DOI: 10.1007/s00795-007-0388-1
975  Shibata MA, Morimoto J, Doi H, Morishima S, Naka M, Otsuki Y.  Electrogene therapy using endostatin, with or without suicide gene therapy, suppresses murine mammary tumor growth and metastasis  Cancer Gene Ther  2007  14(3):268-78  PubMed ID: 17096028   DOI: 10.1038/sj.cgt.7701009
1004  Shibata MA, Morimoto J, Ito Y, Kusakabe K, Otsuki Y.  Experimental gene therapy in mammary and urinary bladder cancer using electrogene transfer  Med Electron Microsc  2004  37(4):216-24  PubMed ID: 15614446   DOI: 10.1007/s00795-004-0247-2
1001  Shibata MA, Morimoto J, Otsuki Y.  Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation  Cancer Gene Ther  2002  9(1):16-27  PubMed ID: 11916241   DOI: 10.1038/sj.cgt.7700415
1432  Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L  New murine mammary tumor cell lines.  In Vitro Cell Dev Biol  1991  27A:349-51  PubMed ID: 2071538   DOI: 10.1007/BF02630952
1003  Kiyozuka Y, Asai A, Senzaki H, Uemura Y, Nakashima A, Morimoto J, Matsuzawa A, Tsubura A.  Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression  Int J Mol Med  1988  2(4):437-44  PubMed ID: 9857231   DOI: 10.3892/ijmm.2.4.437

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User's Publication
12491  Seo I, Cho JH, Lee MH, Park WJ, Kwon SY, Lee JH.  Clinical and Prognostic Value of Human Mammary Tumor Virus in Korean Patients with Breast Carcinoma  Ann Clin Lab Sci  2019  49(2):171-174  PubMed ID: 31028060  
10266  San TH, Fujisawa M, Fushimi S, Yoshimura T, Ohara T, Soe L, Min NW, Kyaw O, Yang X, Matsukawa A.  Low prevalence of human mammary tumor virus (HMTV) in breast cancer patients from Myanmar.  Infect. Agents Cancer  2017  12:20  PubMed ID: 28413435   DOI: 10.1186/s13027-017-0130-0
14650  akano N, Tsuchiya Y, Kako K, Umezaki K, Sano K, Ikeno S, Otsuka E, Shigeta M, Nakagawa A, Sakata N, Itoh F, Nakano Y, Iemura SI, van Dinther M, Natsume T, Ten Dijke P, Itoh S.  TMED10 Protein Interferes with Transforming Growth Factor (TGF)-β Signaling by Disrupting TGF-β Receptor Complex Formation  J Biol Chem  2017  292(10):4099-4112.  PubMed ID: 28115518   DOI: 10.1074/jbc.M116.769109
14659  Okita Y, Kimura M, Xie R, Chen C, Shen LT, Kojima Y, Suzuki H, Muratani M, Saitoh M, Semba K, Heldin CH, Kato M.  The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB  Sci Signal  2017  10(474):eaak9397  PubMed ID: 28400538   DOI: 10.1126/scisignal.aak9397
7147  Hiraga T, Ito S, Nakamura H.  EpCAM expression in breast cancer cells is associated with enhanced bone metastasis formation.  Int. J. Cancer  2016  138:1698-708  PubMed ID: 26576938   DOI: 10.1002/ijc.29921
8619  Lee TL, Li Y, Alba D, Vong QP, Wu SM, Baxendale V, Rennert OM, Lau YF, Chan WY.  Developmental staging of male murine embryonic gonad by SAGE analysis.  J Genet Genomics  2009  36:215-27  PubMed ID: 19376482   DOI: 10.1016/S1673-8527(08)60109-5
3952  Akatsu T, Ono K, Katayama Y, Tamura T, Nishikawa M, Kugai N, Yamamoto M, Nagata N.  The mouse mammary tumor cell line, MMT060562, produces prostaglandin E2 and leukemia inhibitory factor and supports osteoclast formation in vitro via a stromal cell-dependent pathway.  J Bone Miner Res  1998  13(3):400-8  PubMed ID: 9525340   DOI: 10.1359/jbmr.1998.13.3.400
18041  Ding Y, Tan W, Hu R, Chen W, Hou Y.  Construction of a novel fusion protein harboring mouse interferon gamma and epidermal growth factor receptor binding domain and enhancement of its antitumor activity  Sci China C Life Sci  1997  40(3):293-300  PubMed ID: 18726330   DOI: 10.1007/BF02879090



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