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Cell No. : Cell Name
RCB0524 : HFL-AE-I  update : 2022/03/16
CommentHuman fibroblasts derived from lung of anencephalic fetus. 46XY,17p+ or 17ph+
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 15 weeks embryo
Tissue embryo/fetus, lung
Disease name anencephalia
Classification normal
Year of origin 1990
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4636
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(2),46(44),47(1),91(1),92(2)
STR(human) OK
Reference information Reference 0
User's Publication 0


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