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細胞番号 : 細胞名
RCB0523 : HFL-III  update : 2022/03/16
細胞特性(Comment:英)Human fibroblasts derived from fetal lung (11 weekly age).
細胞特性(日)ヒト線維芽細胞。日本人11週齢胎児肺由来。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ishiwata, Isamu
樹立者 Ishiwata, Isamu
寄託日 1990
動物種 human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Unknown
11 weeks embryo
採取組織 embryo/fetus, lung
細胞分類 normal
初代培養日 1990
細胞寿命 finite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_K103
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 HamF12 + 15% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 4 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 45-92(50) : 45(2),46(46),48(1),92(1)
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 23件
利用者成果(日) 0件

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Reference(英)

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利用者成果(英)
15414  Natsumi Saito, Akiko Kikuchi, Mutsuo Yamaya, Xue Deng, Mitsuru Sugawara, Shin Takayama, Ryoichi Nagatomi, Tadashi Ishii  Kakkonto Inhibits Cytokine Production Induced by Rhinovirus Infection in Primary Cultures of Human Nasal Epithelial Cells  Frontiers in Pharmacology  2021    PubMed ID: 34531740   DOI: 10.3389/fphar.2021.687818
10489  Yamaya M, Nomura K, Arakawa K, Sugawara M, Deng X, Lusamba Kalonji N, Nishimura H, Yamada M, Nagatomi R, Kawase T.  Clarithromycin decreases rhinovirus replication and cytokine production in nasal epithelial cells from subjects with bronchial asthma: effects on IL-6, IL-8 and IL-33.  Arch. Pharm. Res.  2020  43(5):526-539  PubMed ID: 28861755   DOI: 10.1007/s12272-017-0950-x
13860  Suzuki M, Yasuda N, Kitamura H.  Lethal and mutagenic bystander effects in human fibroblast cell cultures subjected to low-energy-carbon ions  Int J Radiat Biol  2020  96(2):179-186  PubMed ID: 31633439   DOI: 10.1080/09553002.2020.1683637
11514  Deng X, Yasuda H, Sasaki T, Yamaya M.  Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells.  Tohoku J. Exp. Med.  2019    PubMed ID: 30971638   DOI: 10.1620/tjem.247.215
12920  Yamaya M, Nomura K, Arakawa K, Nishimura H, Lusamba Kalonji N, Kubo H, Nagatomi R, Kawase T.  Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM-1 levels and endosomal acidification and is inhibited by L-carbocisteine  Immun Inflamm Dis  2016  4(2):166-181  PubMed ID: 27957326   DOI: 10.1002/iid3.102
7183  Lusamba Kalonji N, Nomura K, Kawase T, Ota C, Kubo H, Sato T, Yanagisawa T, Sunazuka T, ?mura S, Yamaya M.  The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells.  Physiol Rep  2015  3:  PubMed ID: 26462747   DOI: 10.14814/phy2.12557
7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8
15061  Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, Fujimori A, Okayasu R.  The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells  Cancer Med  2015  4(3):426-36  PubMed ID: 25582113   DOI: 10.1002/cam4.377
15076  Otomaru H, Kamigaki T, Tamaki R, Opinion J, Santo A, Daya E, Okamoto M, Saito M, Tallo V, Lupisan S, Suzuki A, Oshitani H.  Influenza and other respiratory viruses detected by influenza-like illness surveillance in Leyte Island, the Philippines, 2010-2013  PLoS One  2015  10(4):e0123755  PubMed ID: 25893441   DOI: 10.1371/journal.pone.0123755
15143  Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, Hirayama R, Matsumoto Y, Yajima H, Yu D.  The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function  DNA Repair (Amst)  2015  25:72-83  PubMed ID: 25497328   DOI: 10.1016/j.dnarep.2014.11.004
8094  Yamaya M, Nishimura H, Hatachi Y, Yoshida M, Fujiwara H, Asada M, Nakayama K, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.  Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells.  Eur. J. Pharmacol.  2011  650:431-44  PubMed ID: 20940011   DOI: 10.1016/j.ejphar.2010.09.056
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504
12267  Ryuichi Okayasu, Maki Okada, Atsushi Okabe, Miho Noguchi, Kaoru Takakura, Sentaro Takahashi  Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway  Radiat Res  2006  165(1):59-67.  PubMed ID: 16392963   DOI: 10.1667/rr3489.1
3368  Fujimori, Akira, Okayasu, Ryuichi, Ishihara, Hiroshi, Yoshida, Satoshi, Eguchi-Kasai, Kiyomi, Nojima, Kumie, Ebisawa, Satoru, Takahashi, Sentaro  Extremely low dose ionizing radiation up-regulates CXC chemokines in normal human fibroblasts.  Cancer Res  2005  65:10159-63  PubMed ID: 16287999   DOI: 10.1158/0008-5472.CAN-05-2015
3168  Mizuta K, Abiko C, Murata T, Matsuzaki Y, Itagaki T, Sanjoh K, Sakamoto M, Hongo S, Murayama S, Hayasaka K  Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003.  J Clin Microbiol  2005  43:6171-5  PubMed ID: 16333123   DOI: 10.1128/JCM.43.12.6171-6175.2005
3036  Mizuta K, Itagaki T, Abiko C, Murata T, Takahashi T, Murayama S  Epidemics of two Victoria and Yamagata influenza B lineages in Yamagata, Japan.  Epidemiol Infect  2004  132:721-6  PubMed ID: 15310174   DOI: 10.1017/s0950268804002237
17340  Okada M, Saito S, Okayasu R.  Facilitated detection of chromosome break and repair at low levels of ionizing radiation by addition of wortmannin to G1-type PCC fusion incubation  Mutat Res  2004  562(1-2):11-7  PubMed ID: 15279826   DOI: 10.1016/j.mrgentox.2004.04.004
3035  Mizuta, Katsumi, Katsushima, Noriko, Ito, Sueshi, Sanjoh, Kanako, Murata, Toshio, Abiko, Chieko, Murayama, Shoko  A rare appearance of influenza A(H1N2) as a reassortant in a community such as Yamagata where A(H1N1) and A(H3N2) co-circulate.  Microbiol Immunol  2003  47:359-61  PubMed ID: 12825897   DOI: 10.1111/j.1348-0421.2003.tb03407.x
2663  Mizuta, Katsumi, Abiko, Chieko, Goto, Hiroko, Murata, Toshio, Murayama, Shoko  Enterovirus isolation from children with acute respiratory infections and presumptive identification by a modified microplate method.  Int J Infect Dis  2003  7:138-42  PubMed ID: 12839716   DOI: 10.1016/s1201-9712(03)90010-5
3034  Mizuta, Katsumi, Abiko, Chieko, Murata, Toshio, Itagaki, Tsutomu, Katsushima, Noriko, Akiba, Tomoharu, Sakamoto, Michiyo, Ootani, Katsumi, Murayama, Shoko  Re-emergence of echovirus type 13 infections in 2002 in Yamagata, Japan.  J Infect  2003  47:243-7  PubMed ID: 12963387   DOI: 10.1016/s0163-4453(03)00081-1
2478  Suzuki M, Kase Y, Yamaguchi H, Kanai T, Ando K  Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in Chiba (HIMAC) carbon-ion beams.  Int J Radiat Oncol Biol Phys  2000  48:241-50  PubMed ID: 10924995   DOI: 10.1016/s0360-3016(00)00568-x
2477  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell killing and residual chromatin breaks measured by PCC in six human cell lines irradiated with different radiation types.  Int J Radiat Biol  2000  76:1189-96  PubMed ID: 10993630   DOI: 10.1080/09553000050134429
2357  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.  Adv Space Res  1998  22:561-8  PubMed ID: 11542786   DOI: 10.1016/s0273-1177(98)00078-7

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