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Cell No. : Cell Name
RCB0523 : HFL-III  update : 2022/03/16
CommentHuman fibroblasts derived from fetal lung (11 weekly age).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 11 weeks embryo
Tissue embryo/fetus, lung
Classification normal
Year of origin 1990
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_K103
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(2),46(46),48(1),92(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 22


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Reference

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User's Publication
15414  Natsumi Saito, Akiko Kikuchi, Mutsuo Yamaya, Xue Deng, Mitsuru Sugawara, Shin Takayama, Ryoichi Nagatomi, Tadashi Ishii  Kakkonto Inhibits Cytokine Production Induced by Rhinovirus Infection in Primary Cultures of Human Nasal Epithelial Cells  Frontiers in Pharmacology  2021      DOI: 10.3389/fphar.2021.687818
10489  Yamaya M, Nomura K, Arakawa K, Sugawara M, Deng X, Lusamba Kalonji N, Nishimura H, Yamada M, Nagatomi R, Kawase T.  Clarithromycin decreases rhinovirus replication and cytokine production in nasal epithelial cells from subjects with bronchial asthma: effects on IL-6, IL-8 and IL-33.  Arch. Pharm. Res.  2020  43(5):526-539  PubMed ID: 28861755   DOI: 10.1007/s12272-017-0950-x
13860  Suzuki M, Yasuda N, Kitamura H.  Lethal and mutagenic bystander effects in human fibroblast cell cultures subjected to low-energy-carbon ions  Int J Radiat Biol  2020  96(2):179-186  PubMed ID: 31633439   DOI: 10.1080/09553002.2020.1683637
11514  Deng X, Yasuda H, Sasaki T, Yamaya M.  Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells.  Tohoku J. Exp. Med.  2019    PubMed ID: 30971638   DOI: 10.1620/tjem.247.215
12920  Yamaya M, Nomura K, Arakawa K, Nishimura H, Lusamba Kalonji N, Kubo H, Nagatomi R, Kawase T.  Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM-1 levels and endosomal acidification and is inhibited by L-carbocisteine  Immun Inflamm Dis  2016  4(2):166-181  PubMed ID: 27957326   DOI: 10.1002/iid3.102
7183  Lusamba Kalonji N, Nomura K, Kawase T, Ota C, Kubo H, Sato T, Yanagisawa T, Sunazuka T, ?mura S, Yamaya M.  The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells.  Physiol Rep  2015  3:  PubMed ID: 26462747   DOI: 10.14814/phy2.12557
7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8
15061  Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, Fujimori A, Okayasu R.  The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells  Cancer Med  2015  4(3):426-36  PubMed ID: 25582113   DOI: 10.1002/cam4.377
15076  Otomaru H, Kamigaki T, Tamaki R, Opinion J, Santo A, Daya E, Okamoto M, Saito M, Tallo V, Lupisan S, Suzuki A, Oshitani H.  Influenza and other respiratory viruses detected by influenza-like illness surveillance in Leyte Island, the Philippines, 2010-2013  PLoS One  2015  10(4):e0123755  PubMed ID: 25893441   DOI: 10.1371/journal.pone.0123755
15143  Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, Hirayama R, Matsumoto Y, Yajima H, Yu D.  The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function  DNA Repair (Amst)  2015  25:72-83  PubMed ID: 25497328   DOI: 10.1016/j.dnarep.2014.11.004
8094  Yamaya M, Nishimura H, Hatachi Y, Yoshida M, Fujiwara H, Asada M, Nakayama K, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.  Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells.  Eur. J. Pharmacol.  2011  650:431-44  PubMed ID: 20940011   DOI: 10.1016/j.ejphar.2010.09.056
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504
12267  Ryuichi Okayasu, Maki Okada, Atsushi Okabe, Miho Noguchi, Kaoru Takakura, Sentaro Takahashi  Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway  Radiat Res  2006  165(1):59-67.  PubMed ID: 16392963   DOI: 10.1667/rr3489.1
3368  Fujimori, Akira, Okayasu, Ryuichi, Ishihara, Hiroshi, Yoshida, Satoshi, Eguchi-Kasai, Kiyomi, Nojima, Kumie, Ebisawa, Satoru, Takahashi, Sentaro  Extremely low dose ionizing radiation up-regulates CXC chemokines in normal human fibroblasts.  Cancer Res  2005  65:10159-63  PubMed ID: 16287999   DOI: 10.1158/0008-5472.CAN-05-2015
3168  Mizuta K, Abiko C, Murata T, Matsuzaki Y, Itagaki T, Sanjoh K, Sakamoto M, Hongo S, Murayama S, Hayasaka K  Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003.  J Clin Microbiol  2005  43:6171-5  PubMed ID: 16333123   DOI: 10.1128/JCM.43.12.6171-6175.2005
3036  Mizuta K, Itagaki T, Abiko C, Murata T, Takahashi T, Murayama S  Epidemics of two Victoria and Yamagata influenza B lineages in Yamagata, Japan.  Epidemiol Infect  2004  132:721-6  PubMed ID: 15310174   DOI: 10.1017/s0950268804002237
3035  Mizuta, Katsumi, Katsushima, Noriko, Ito, Sueshi, Sanjoh, Kanako, Murata, Toshio, Abiko, Chieko, Murayama, Shoko  A rare appearance of influenza A(H1N2) as a reassortant in a community such as Yamagata where A(H1N1) and A(H3N2) co-circulate.  Microbiol Immunol  2003  47:359-61  PubMed ID: 12825897   DOI: 10.1111/j.1348-0421.2003.tb03407.x
2663  Mizuta, Katsumi, Abiko, Chieko, Goto, Hiroko, Murata, Toshio, Murayama, Shoko  Enterovirus isolation from children with acute respiratory infections and presumptive identification by a modified microplate method.  Int J Infect Dis  2003  7:138-42  PubMed ID: 12839716   DOI: 10.1016/s1201-9712(03)90010-5
3034  Mizuta, Katsumi, Abiko, Chieko, Murata, Toshio, Itagaki, Tsutomu, Katsushima, Noriko, Akiba, Tomoharu, Sakamoto, Michiyo, Ootani, Katsumi, Murayama, Shoko  Re-emergence of echovirus type 13 infections in 2002 in Yamagata, Japan.  J Infect  2003  47:243-7  PubMed ID: 12963387   DOI: 10.1016/s0163-4453(03)00081-1
2478  Suzuki M, Kase Y, Yamaguchi H, Kanai T, Ando K  Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in Chiba (HIMAC) carbon-ion beams.  Int J Radiat Oncol Biol Phys  2000  48:241-50  PubMed ID: 10924995   DOI: 10.1016/s0360-3016(00)00568-x
2477  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell killing and residual chromatin breaks measured by PCC in six human cell lines irradiated with different radiation types.  Int J Radiat Biol  2000  76:1189-96  PubMed ID: 10993630   DOI: 10.1080/09553000050134429
2357  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.  Adv Space Res  1998  22:561-8  PubMed ID: 11542786   DOI: 10.1016/s0273-1177(98)00078-7



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