Cell No. : Cell Name RCB0523 : HFL-III  update : 2022/03/16
Comment			Human fibroblasts derived from fetal lung (11 weekly age).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ishiwata, Isamu
		Originator	Ishiwata, Isamu
		Year of deposit	1990
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Unknown
		Age at sampling	11 weeks embryo
		Tissue	embryo/fetus, lung
		Classification	normal
		Year of origin	1990
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_K103
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF12 + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		45-92(50) : 45(2),46(46),48(1),92(1)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	30

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Reference

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User's Publication

15414  Natsumi Saito, Akiko Kikuchi, Mutsuo Yamaya, Xue Deng, Mitsuru Sugawara, Shin Takayama, Ryoichi Nagatomi, Tadashi Ishii  Kakkonto Inhibits Cytokine Production Induced by Rhinovirus Infection in Primary Cultures of Human Nasal Epithelial Cells  Frontiers in Pharmacology  2021  12:687818  PubMed ID: 34531740   DOI: 10.3389/fphar.2021.687818

10489  Yamaya M, Nomura K, Arakawa K, Sugawara M, Deng X, Lusamba Kalonji N, Nishimura H, Yamada M, Nagatomi R, Kawase T.  Clarithromycin decreases rhinovirus replication and cytokine production in nasal epithelial cells from subjects with bronchial asthma: effects on IL-6, IL-8 and IL-33.  Arch. Pharm. Res.  2020  43(5):526-539  PubMed ID: 28861755   DOI: 10.1007/s12272-017-0950-x

13860  Suzuki M, Yasuda N, Kitamura H.  Lethal and mutagenic bystander effects in human fibroblast cell cultures subjected to low-energy-carbon ions  Int J Radiat Biol  2020  96(2):179-186  PubMed ID: 31633439   DOI: 10.1080/09553002.2020.1683637

11514  Deng X, Yasuda H, Sasaki T, Yamaya M.  Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells.  Tohoku J. Exp. Med.  2019    PubMed ID: 30971638   DOI: 10.1620/tjem.247.215

4344  Li HK, Hasegawa S, Nakajima NI, Morokoshi Y, Minegishi K, Nagatsu K.  Targeted cancer cell ablation in mice by an α-particle-emitting astatine-211-labeled antibody against major histocompatibility complex class I chain-related protein A and B.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 30409432   DOI: 10.1016/j.bbrc.2018.10.157

12920  Yamaya M, Nomura K, Arakawa K, Nishimura H, Lusamba Kalonji N, Kubo H, Nagatomi R, Kawase T.  Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM-1 levels and endosomal acidification and is inhibited by L-carbocisteine  Immun Inflamm Dis  2016  4(2):166-181  PubMed ID: 27957326   DOI: 10.1002/iid3.102

7183  Lusamba Kalonji N, Nomura K, Kawase T, Ota C, Kubo H, Sato T, Yanagisawa T, Sunazuka T, ?mura S, Yamaya M.  The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells.  Physiol Rep  2015  3:  PubMed ID: 26462747   DOI: 10.14814/phy2.12557

7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8

15061  Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, Fujimori A, Okayasu R.  The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells  Cancer Med  2015  4(3):426-36  PubMed ID: 25582113   DOI: 10.1002/cam4.377

15076  Otomaru H, Kamigaki T, Tamaki R, Opinion J, Santo A, Daya E, Okamoto M, Saito M, Tallo V, Lupisan S, Suzuki A, Oshitani H.  Influenza and other respiratory viruses detected by influenza-like illness surveillance in Leyte Island, the Philippines, 2010-2013  PLoS One  2015  10(4):e0123755  PubMed ID: 25893441   DOI: 10.1371/journal.pone.0123755

15143  Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, Hirayama R, Matsumoto Y, Yajima H, Yu D.  The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function  DNA Repair (Amst)  2015  25:72-83  PubMed ID: 25497328   DOI: 10.1016/j.dnarep.2014.11.004

19327  Yamaya M, Nishimura H, Nadine L, Kubo H, Ryoichi N.  Tulobuterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells  Physiol Rep  2013  1(3):e00041  PubMed ID: 24303127   DOI: 10.1002/phy2.41

18727  Yamaya M, Nishimura H, Hatachi Y, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.  Inhibitory effects of tiotropium on rhinovirus infection in human airway epithelial cells  Eur Respir J  2012  40(1):122-32.  PubMed ID: 22362848   DOI: 10.1183/09031936.00065111

19096  Yamaya M, Nishimura H, Hatachi Y, Yasuda H, Deng X, Sasaki T, Mizuta K, Kubo H, Nagatomi R.  Levofloxacin inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells  Antimicrob Agents Chemother  2012  56(8):4052-61.  PubMed ID: 22585227   DOI: 10.1128/AAC.00259-12

8094  Yamaya M, Nishimura H, Hatachi Y, Yoshida M, Fujiwara H, Asada M, Nakayama K, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.  Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells.  Eur. J. Pharmacol.  2011  650:431-44  PubMed ID: 20940011   DOI: 10.1016/j.ejphar.2010.09.056

19135  Yao M, Yao D, Yamaguchi M, Chida J, Yao D, Kido H.  Bezafibrate upregulates carnitine palmitoyltransferase II expression and promotes mitochondrial energy crisis dissipation in fibroblasts of patients with influenza-associated encephalopathy  Mol Genet Metab  2011  104(3):265-72  PubMed ID: 21816645   DOI: 10.1016/j.ymgme.2011.07.009

18375  Sekine E, Okada M, Matsufuji N, Yu D, Furusawa Y, Okayasu R.  High LET heavy ion radiation induces lower numbers of initial chromosome breaks with minimal repair than low LET radiation in normal human cells  Mutat Res  2008  652(1):95-101  PubMed ID: 18314380   DOI: 10.1016/j.mrgentox.2008.01.003

14891  Fujimori A, Yaoi T, Ogi H, Wang B, Suetomi K, Sekine E, Yu D, Kato T, Takahashi S, Okayasu R, Itoh K, Fushiki S.  Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans  Biochem Biophys Res Commun  2008  369(3):953-7  PubMed ID: 18331833   DOI: 10.1016/j.bbrc.2008.02.149

3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

12267  Ryuichi Okayasu, Maki Okada, Atsushi Okabe, Miho Noguchi, Kaoru Takakura, Sentaro Takahashi  Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway  Radiat Res  2006  165(1):59-67.  PubMed ID: 16392963   DOI: 10.1667/rr3489.1

3368  Fujimori, Akira, Okayasu, Ryuichi, Ishihara, Hiroshi, Yoshida, Satoshi, Eguchi-Kasai, Kiyomi, Nojima, Kumie, Ebisawa, Satoru, Takahashi, Sentaro  Extremely low dose ionizing radiation up-regulates CXC chemokines in normal human fibroblasts.  Cancer Res  2005  65:10159-63  PubMed ID: 16287999   DOI: 10.1158/0008-5472.CAN-05-2015

3168  Mizuta K, Abiko C, Murata T, Matsuzaki Y, Itagaki T, Sanjoh K, Sakamoto M, Hongo S, Murayama S, Hayasaka K  Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003.  J Clin Microbiol  2005  43:6171-5  PubMed ID: 16333123   DOI: 10.1128/JCM.43.12.6171-6175.2005

3036  Mizuta K, Itagaki T, Abiko C, Murata T, Takahashi T, Murayama S  Epidemics of two Victoria and Yamagata influenza B lineages in Yamagata, Japan.  Epidemiol Infect  2004  132:721-6  PubMed ID: 15310174   DOI: 10.1017/s0950268804002237

17340  Okada M, Saito S, Okayasu R.  Facilitated detection of chromosome break and repair at low levels of ionizing radiation by addition of wortmannin to G1-type PCC fusion incubation  Mutat Res  2004  562(1-2):11-7  PubMed ID: 15279826   DOI: 10.1016/j.mrgentox.2004.04.004

3035  Mizuta, Katsumi, Katsushima, Noriko, Ito, Sueshi, Sanjoh, Kanako, Murata, Toshio, Abiko, Chieko, Murayama, Shoko  A rare appearance of influenza A(H1N2) as a reassortant in a community such as Yamagata where A(H1N1) and A(H3N2) co-circulate.  Microbiol Immunol  2003  47:359-61  PubMed ID: 12825897   DOI: 10.1111/j.1348-0421.2003.tb03407.x

2663  Mizuta, Katsumi, Abiko, Chieko, Goto, Hiroko, Murata, Toshio, Murayama, Shoko  Enterovirus isolation from children with acute respiratory infections and presumptive identification by a modified microplate method.  Int J Infect Dis  2003  7:138-42  PubMed ID: 12839716   DOI: 10.1016/s1201-9712(03)90010-5

3034  Mizuta, Katsumi, Abiko, Chieko, Murata, Toshio, Itagaki, Tsutomu, Katsushima, Noriko, Akiba, Tomoharu, Sakamoto, Michiyo, Ootani, Katsumi, Murayama, Shoko  Re-emergence of echovirus type 13 infections in 2002 in Yamagata, Japan.  J Infect  2003  47:243-7  PubMed ID: 12963387   DOI: 10.1016/s0163-4453(03)00081-1

2478  Suzuki M, Kase Y, Yamaguchi H, Kanai T, Ando K  Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in Chiba (HIMAC) carbon-ion beams.  Int J Radiat Oncol Biol Phys  2000  48:241-50  PubMed ID: 10924995   DOI: 10.1016/s0360-3016(00)00568-x

2477  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell killing and residual chromatin breaks measured by PCC in six human cell lines irradiated with different radiation types.  Int J Radiat Biol  2000  76:1189-96  PubMed ID: 10993630   DOI: 10.1080/09553000050134429

2357  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.  Adv Space Res  1998  22:561-8  PubMed ID: 11542786   DOI: 10.1016/s0273-1177(98)00078-7