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Cell No. : Cell Name
RCB0523 : HFL-III  update : 2018/07/06
CommentNormal embryonic lung fibroblast
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 11 weeks embryo
Tissue embryo/fetus, lung
Classification normal
Year of origin 1990
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(2),46(46),48(1),92(1)
deposit info
lot info
Reference information Reference 0
User's Publication 13

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User's Publication
11514  Deng X, Yasuda H, Sasaki T, Yamaya M.  Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells.  Tohoku J. Exp. Med.  2019    PubMed ID: 30971638
10489  Yamaya M, Nomura K, Arakawa K, Sugawara M, Deng X, Lusamba Kalonji N, Nishimura H, Yamada M, Nagatomi R, Kawase T.  Clarithromycin decreases rhinovirus replication and cytokine production in nasal epithelial cells from subjects with bronchial asthma: effects on IL-6, IL-8 and IL-33.  Arch. Pharm. Res.  2017    PubMed ID: 28861755
7183  Lusamba Kalonji N, Nomura K, Kawase T, Ota C, Kubo H, Sato T, Yanagisawa T, Sunazuka T, ?mura S, Yamaya M.  The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells.  Physiol Rep  2015  3:  PubMed ID: 26462747
8094  Yamaya M, Nishimura H, Hatachi Y, Yoshida M, Fujiwara H, Asada M, Nakayama K, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.  Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells.  Eur. J. Pharmacol.  2010  650:431-44  PubMed ID: 20940011
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773
3168  Mizuta K, Abiko C, Murata T, Matsuzaki Y, Itagaki T, Sanjoh K, Sakamoto M, Hongo S, Murayama S, Hayasaka K  Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003.  J Clin Microbiol  2005  43:6171-5  PubMed ID: 16333123
3036  Mizuta K, Itagaki T, Abiko C, Murata T, Takahashi T, Murayama S  Epidemics of two Victoria and Yamagata influenza B lineages in Yamagata, Japan.  Epidemiol Infect  2004  132:721-6  PubMed ID: 15310174
3035  Mizuta, Katsumi, Katsushima, Noriko, Ito, Sueshi, Sanjoh, Kanako, Murata, Toshio, Abiko, Chieko, Murayama, Shoko  A rare appearance of influenza A(H1N2) as a reassortant in a community such as Yamagata where A(H1N1) and A(H3N2) co-circulate.  Microbiol Immunol  2003  47:359-61  PubMed ID: 12825897
2663  Mizuta, Katsumi, Abiko, Chieko, Goto, Hiroko, Murata, Toshio, Murayama, Shoko  Enterovirus isolation from children with acute respiratory infections and presumptive identification by a modified microplate method.  Int J Infect Dis  2003  7:138-42  PubMed ID: 12839716
3034  Mizuta, Katsumi, Abiko, Chieko, Murata, Toshio, Itagaki, Tsutomu, Katsushima, Noriko, Akiba, Tomoharu, Sakamoto, Michiyo, Ootani, Katsumi, Murayama, Shoko  Re-emergence of echovirus type 13 infections in 2002 in Yamagata, Japan.  J Infect  2003  47:243-7  PubMed ID: 12963387
2478  Suzuki M, Kase Y, Yamaguchi H, Kanai T, Ando K  Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in Chiba (HIMAC) carbon-ion beams.  Int J Radiat Oncol Biol Phys  2000  48:241-50  PubMed ID: 10924995
2477  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell killing and residual chromatin breaks measured by PCC in six human cell lines irradiated with different radiation types.  Int J Radiat Biol  2000  76:1189-96  PubMed ID: 10993630
2357  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.  Adv Space Res  1998  22:561-8  PubMed ID: 11542786

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