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Cell No. : Cell Name
RCB0521 : HFL-I  update : 2020/09/24
CommentNormal embryonic lung fibroblast
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 11 weeks embryo
Tissue embryo/fetus, lung
Classification normal
Year of origin 1990
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(1),45(1),46(47),92(1)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 18


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Reference

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User's Publication
6904  Kuse N, Kamio K, Azuma A, Matsuda K, Inomata M, Usuki J, Morinaga A, Tanaka T, Kashiwada T, Atsumi K, Hayashi H, Saito Y, Seike M, Gemma A.  Exosome-derived microRNA-22 ameliorates pulmonary fibrosis by regulating fibroblast-to-myofibroblast differentiation both in vitro and in vivo.  J Nippon Med Sch.  2020  87(3):118-128  PubMed ID: 31776321
10800  Watanabe K, Otabe K, Shimizu N, Komori K, Mizuno M, Katano H, Koga H, Sekiya I.  High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow.  Stem Cell Res Ther  2018  9:80  PubMed ID: 29587847
4485  Kuwabara Y, Kobayashi T, D'Alessandro-Gabazza CN, Toda M, Yasuma T, Nishihama K, Takeshita A, Fujimoto H, Nagao M, Fujisawa T, Gabazza EC.  Role of Matrix Metalloproteinase-2 in Eosinophil-Mediated Airway Remodeling.  Front Immunol  2018  9:2163  PubMed ID: 30294331
12226  Koichiro Kamio, Arata Azuma, Jiro Usuki, Kuniko Matsuda, Minoru Inomata, Nobuhiko Nishijima, Shioto Itakura, Hiroki Hayashi, Takeru Kashiwada, Nariaki Kokuho, Kenichiro Atsumi, Tomoyoshi Yamaguchi, Kazue Fujita, Yoshinobu Saito, Shinji Abe, Kaoru Kubota, Akihiko Gemma  XPLN is modulated by HDAC inhibitors and negatively regulates SPARC expression by targeting mTORC2 in human lung fibroblasts  Pulm Pharmacol Ther  2017  44:61-69  PubMed ID: 28315487
7308  Hiratsuka M, Ueda K, Uno N, Uno K, Fukuhara S, Kurosaki H, Takehara S, Osaki M, Kazuki Y, Kurosawa Y, Nakamura T, Katoh M, Oshimura M.  Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome.  BMC Biotechnol.  2015  15:58  PubMed ID: 26088202
5630  Wada M, Suzuki M, Liu C, Kaneko Y, Fukuda S, Ando K, Matsufuji N.  Modeling the biological response of normal human cells, including repair processes, to fractionated carbon beam irradiation.  J Radiat Res  2013  54(5):798-807  PubMed ID: 23449640
8275  Katoh M, Kazuki Y, Kazuki K, Kajitani N, Takiguchi M, Nakayama Y, Nakamura T, Oshimura M.  Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer.  BMC Biotechnol.  2010  10:37  PubMed ID: 20444293
8764  Shitara S, Kakeda M, Nagata K, Hiratsuka M, Sano A, Osawa K, Okazaki A, Katoh M, Kazuki Y, Oshimura M, Tomizuka K.  Telomerase-mediated life-span extension of human primary fibroblasts by human artificial chromosome (HAC) vector.  Biochem. Biophys. Res. Commun.  2008  369:807-11  PubMed ID: 18328257
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773
3168  Mizuta K, Abiko C, Murata T, Matsuzaki Y, Itagaki T, Sanjoh K, Sakamoto M, Hongo S, Murayama S, Hayasaka K  Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003.  J Clin Microbiol  2005  43:6171-5  PubMed ID: 16333123
2824  Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K  Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.  Gene Ther  2005  12:852-6  PubMed ID: 15750614
2722  Matsui S, Matsumoto H, Sonoda Y, Ando K, Aizu-Yokota E, Sato T, Kasahara T  Glycyrrhizin and related compounds down-regulate production of inflammatory chemokines IL-8 and eotaxin 1 in a human lung fibroblast cell line  Int Immunopharmacol  2004  15:1633-1644  PubMed ID: 15454116
3036  Mizuta K, Itagaki T, Abiko C, Murata T, Takahashi T, Murayama S  Epidemics of two Victoria and Yamagata influenza B lineages in Yamagata, Japan.  Epidemiol Infect  2004  132:721-6  PubMed ID: 15310174
3035  Mizuta, Katsumi, Katsushima, Noriko, Ito, Sueshi, Sanjoh, Kanako, Murata, Toshio, Abiko, Chieko, Murayama, Shoko  A rare appearance of influenza A(H1N2) as a reassortant in a community such as Yamagata where A(H1N1) and A(H3N2) co-circulate.  Microbiol Immunol  2003  47:359-61  PubMed ID: 12825897
2663  Mizuta, Katsumi, Abiko, Chieko, Goto, Hiroko, Murata, Toshio, Murayama, Shoko  Enterovirus isolation from children with acute respiratory infections and presumptive identification by a modified microplate method.  Int J Infect Dis  2003  7:138-42  PubMed ID: 12839716
3034  Mizuta, Katsumi, Abiko, Chieko, Murata, Toshio, Itagaki, Tsutomu, Katsushima, Noriko, Akiba, Tomoharu, Sakamoto, Michiyo, Ootani, Katsumi, Murayama, Shoko  Re-emergence of echovirus type 13 infections in 2002 in Yamagata, Japan.  J Infect  2003  47:243-7  PubMed ID: 12963387
5553  Kobayashi H, Higashiyama M, Minamigawa K, Tanisaka K, Takano T, Yokouchi H, Kodama K, Hata T.  Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification.  Jpn J Cancer Res  2001  92(2):203-10  PubMed ID: 11223550
5537  Takaoka AS, Yamada T, Gotoh M, Kanai Y, Imai K, Hirohashi S.  Cloning and characterization of the human beta4-integrin gene promoter and enhancers.  J Biol Chem  1998  273(50):33848-55  PubMed ID: 9837976



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