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Cell No. : Cell Name
RCB0520 : WR216  update : 2022/03/16
CommentAPRT(-) EB transformed B cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kamatani, Naoyuki
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue blood
Disease name urolithiasis
Genetics APRT^(*)Q0/APRT^(*)Q0
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_5606
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Chromosome mode 39-80(50) : /42(1),43(1),44(2),45(7),346(34),80(1)
STR(human) OK
Reference information Reference 3
User's Publication 0


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Reference
1284  Kamatani N, Kuroshima S, Terai C, Hidaka Y, Palella, T D, Nishioka K  Detection of an amino acid substitution in the mutant enzyme for a special type of adenine phosphoribosyltransferase (APRT) deficiency by sequence-specific protein cleavage.  Am Jhum Genet  1989  45:325-331  PubMed ID: 2502918
1283  Hidaka Y, Tarle, S A, Fujimori S, Kamatani N, Kelley, W N, Palella, T D  Human adenine phosphoribosyltransferase deficiency. Demonstration of a single mutant allele common to the Japanese.  J Clin Invest  1988  81:945-50  PubMed ID: 3343350
1282  Nobori T, Kamatani N, Mikanagi K, Nishida Y, Nishioka K  Establishment and characterization of B cell lines from individuals with various types of adenine phosphoribosyltransferase deficiencies.  Biochem Biophys Res Commun  1986  137:998-1005  PubMed ID: 3488062

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