Cell No. : Cell Name
RCB0520 : WR216
update : 2024/01/17
|
| Comment | APRT(-) EB transformed B cell line |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kamatani, Naoyuki
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
blood
|
| Disease name |
urolithiasis
|
| Genetics |
APRT^(*)Q0/APRT^(*)Q0
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_5606
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Virus (HTLV-1) |
|
(-)
|
| Chromosome mode |
|
39-80(50) : /42(1),43(1),44(2),45(7),346(34),80(1)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
1284
Kamatani N, Kuroshima S, Terai C, Hidaka Y, Palella, T D, Nishioka K
Detection of an amino acid substitution in the mutant enzyme for a special type of adenine phosphoribosyltransferase (APRT) deficiency by sequence-specific protein cleavage.
Am Jhum Genet
1989
45:325-331
PubMed ID: 2502918
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1283
Hidaka Y, Tarle, S A, Fujimori S, Kamatani N, Kelley, W N, Palella, T D
Human adenine phosphoribosyltransferase deficiency. Demonstration of a single mutant allele common to the Japanese.
J Clin Invest
1988
81:945-50
PubMed ID: 3343350
DOI: 10.1172/JCI113408
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1282
Nobori T, Kamatani N, Mikanagi K, Nishida Y, Nishioka K
Establishment and characterization of B cell lines from individuals with various types of adenine phosphoribosyltransferase deficiencies.
Biochem Biophys Res Commun
1986
137:998-1005
PubMed ID: 3488062
DOI: 10.1016/0006-291x(86)90324-4
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