Cell No. : Cell Name
RCB0515 : HSKTC
update : 2022/09/30
|
| Comment | Human cell line derived from Krukenberg tumor. Metastasis to ovary of gastric signet ring cell carcinoma. Producing mucin. Expressing CA19-9, CEA, and TPA. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishiwata, Isamu
|
| Originator |
Ishiwata, Isamu
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
46 years
|
| Tissue |
ovary
|
| Disease name |
Krukenberg tumor
|
| Classification |
cancer
|
| Year of origin |
1987
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8234
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF12 + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
87-96(50) : /90(4),91(10),92(12),93(9),94(6),95(2)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
2
|
| User's Publication |
|
17515
Dai Y, Kawaguchi T, Nishio M, Otani J, Tashiro H, Terai Y, Sasaki R, Maehama T, Suzuki A.
The TIGD5 gene located in 8q24 and frequently amplified in ovarian cancers is a tumor suppressor
Genes Cells
2022
27(11):633-642
PubMed ID: 36054307
DOI: 10.1111/gtc.12980
|
|
5888
Kuwabara Y, Yamada T, Yamazaki K, Du WL, Banno K, Aoki D, Sakamoto M.
Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis.
Cancer Sci
2008
99(10):1933-9
PubMed ID: 19016752
DOI: 10.1111/j.1349-7006.2008.00946.x
|
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