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Cell No. : Cell Name
RCB0515 : HSKTC  update : 2022/09/30
CommentHuman cell line derived from Krukenberg tumor. Metastasis to ovary of gastric signet ring cell carcinoma. Producing mucin. Expressing CA19-9, CEA, and TPA.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 46 years
Tissue ovary
Disease name Krukenberg tumor
Classification cancer
Year of origin 1987
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8234
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 87-96(50) : /90(4),91(10),92(12),93(9),94(6),95(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
1199  Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Nozawa S, Ishikawa H  Characterization of Krukenberg tumor cell line, especially the biological relationship between cancer and stromal cells.  Exp Pathol  1990  38:97-108  PubMed ID: 2160890  

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User's Publication
5888  Kuwabara Y, Yamada T, Yamazaki K, Du WL, Banno K, Aoki D, Sakamoto M.  Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis.  Cancer Sci  2008  99(10):1933-9  PubMed ID: 19016752   DOI: 10.1111/j.1349-7006.2008.00946.x



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