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Cell No. : Cell Name
RCB0512 : RCN-H-4  update : 2022/03/16
CommentMetastasis to liver
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Inoue, Yuichiro
Originator Inoue, Yuichiro
Year of deposit 1990
Original cell RCN-9
Animal _rat < Mammals
Strain name F344
Gender Male
Tissue colon
Classification cancer
Year of origin 1988
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5575
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 76-89(50) : /84(10),85(8),86(4),87(10),88(9),89(7)
deposit info
lot info
Reference information Reference 1
User's Publication 4

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1369  Kashima, Yuji  The Establishment of a Rat Colon Cancer Cell Line, ACL-15, with an Analysis of Its Metastatic Properties  Acta Medica et Biologica  1991  39:75-81   

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User's Publication
5369  Yamaki M, Shinozaki K, Sakaguchi T, Meseck M, Ebert O, Ohdan H, Woo SL.  The potential of recombinant vesicular stomatitis virus-mediated virotherapy against metastatic colon cancer.  Int J Mol Med  2013  31(2):299-306  PubMed ID: 23232984   DOI: 10.3892/ijmm.2012.1205
4729  Tamagawa K, Horiuchi T, Wada T, Bannai K, Ando T.  Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer.  Langenbecks Arch Surg  2012  397(3):475-80  PubMed ID: 22207390   DOI: 10.1007/s00423-011-0896-2
8263  Ohsawa I, Murakami T, Guo L, Enosawa S, Ishibashi N, Isaji S, Uemoto S, Kobayashi E.  Chemopreventive anti-cancer agent acyclic retinoid suppresses allogeneic immune responses in rats.  Int. Immunopharmacol.  2010  10:985-9  PubMed ID: 20460176   DOI: 10.1016/j.intimp.2010.04.017
18764  Iwase N, Higuchi T, Gonda T, Kobayashi H, Uetake H, Enomoto M, Sugihara K.  The effect of meloxicam, a selective COX-2 inhibitor, on the microvasculature of small metastatic liver tumors in rats  Jpn J Clin Oncol  2007  37(9):673-8  PubMed ID: 17725981   DOI: 10.1093/jjco/hym081

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