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細胞番号 : 細胞名
RCB0511 : RCN-9  update : 2022/03/16
細胞特性(Comment:英)Metastasis to liver, lung
細胞特性(日)転移モデルに利用されるラット大腸癌。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Inoue, Yuichiro
樹立者 Inoue, Yuichiro
寄託日 1990
動物種 _rat < Mammals
動物系統名 F344
性別 Male
20 weeks
採取組織 colon
細胞分類 cancer
初代培養日 1988
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_5349
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
アイソザイム検査 LD, NP
染色体検査 43-86(50) : 43(48),44(1),86(1)
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 1件
Reference(日) 3件
利用者成果(英) 36件
利用者成果(日) 2件

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Reference(英)
1323  Inoue Y, Kashima Y, Aizawa K, Hatakeyama K  A New Rat Colon Cancer Cell Line Metastasizes Spontaneously : Biologic Characteristics and Chemotherapeutic Response  Jpn J Cancer Res  1991  82:90-97  PubMed ID: 1900274   DOI: 10.1111/j.1349-7006.1991.tb01751.x

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Reference(日)
1205  鹿嶋雄治,井上雄一朗,藍沢喜久雄,畠山勝義,武藤輝一  DMH誘発ラット大腸癌細胞培養株を用いた肝転移モデルとそのin vitro浸潤能    1990     
1203  鹿嶋雄治,井上雄一朗,藍沢喜久雄,片柳憲雄,畠山勝義,武藤輝一  DMH誘発ラット大腸癌培養細胞株RCN-9より得られた肝高転移株の生物学的特性-親株との比較検討-    1990     
1204  井上雄一朗,鹿嶋雄治,須田武保,畠山勝義,武藤輝一  DMH誘発F344ラット大腸癌細胞培養株(RCN-9)の樹立とその性状    1988     

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利用者成果(英)
20089  Taiji R, Nishiofuku H, Tanaka T, Minamiguchi K, Fukuoka Y, Saito N, Taguchi H, Matsumoto T, Marugami N, Hirai T, Kichikawa K.  Useful Parameters in Dynamic Contrast-enhanced Ultrasonography for Identifying Early Response to Chemotherapy in a Rat Liver Tumor Model.  J Clin Imaging Sci  2021  11:15  PubMed ID: 33767907   DOI: 10.25259/JCIS_6_2020
4735  Nishiofuku H, Cortes AC, Ensor JE, Minhaj AA, Polak U, Lopez MS, Kiefer R, Hunt SJ, Kichikawa K, Hicks ME, Gade TP, Avritscher R.  Factors impacting technical success rate of image-guided intra-arterial therapy in rat orthotopic liver tumor model.  Am J Transl Res  2019  11(6):3761-3770  PubMed ID: 31312386  
4715  Imano M, Itoh T, Satou T, Kido A, Tsubaki M, Yasuda A, Kato H, Imamoto H, Nishida S, Furukawa H, Takeyama Y, Okuno K, Shiozaki H.  Establishment of a novel model of peritoneal carcinomatosis of the peritoneal extension type.  Anticancer Res  2013  33(4):1439-46  PubMed ID: 23564784  
7655  Takeda S, Chinda J, Murakami T, Numata A, Iwazu Y, Akimoto T, Hamano Y, Muto S, Takahashi M, Kusano E.  Development of features of glomerulopathy in tumor-bearing rats: a potential model for paraneoplastic glomerulopathy.  Nephrol. Dial. Transplant.  2012  27:1786-92  PubMed ID: 22025112   DOI: 10.1093/ndt/gfr565
8309  Nozaki M, Yoshikawa M, Ishitani K, Kobayashi H, Houkin K, Imai K, Ito Y, Muraki T.  Cysteinyl leukotriene receptor antagonists inhibit tumor metastasis by inhibiting capillary permeability.  Keio J Med  2010  59:10-8  PubMed ID: 20375653   DOI: 10.2302/kjm.59.10
14411  Iida T, Shiba H, Misawa T, Ohashi T, Eto Y, Yanaga K.  Immunogene therapy against colon cancer metastasis using an adenovirus vector expressing CD40 ligand  Surgery  2010  148(5):925-35  PubMed ID: 20378141   DOI: 10.1016/j.surg.2010.02.004
8586  Wang Y, Iguchi K, Ito H, Ookawa K, Kobayashi N, Nakamura R, Goto Y, Sakai M, Ishikawa S, Onizuka M.  Blood flow velocity is reduced in a tumor micro-dissemination in the visceral pleura in anesthetized open-chest rat lung.  In Vivo  2009  23:291-5  PubMed ID: 19414417  
8675  Jin Y, Takegahara Y, Sugawara Y, Matsumura T, Fujinaga Y.  Disruption of the epithelial barrier by botulinum haemagglutinin (HA) proteins - differences in cell tropism and the mechanism of action between HA proteins of types A or B, and HA proteins of type C.  Microbiology (Reading, Engl.)  2009  155:35-45  PubMed ID: 19118344   DOI: 10.1099/mic.0.021246-0
8707  Iida T, Shiba H, Misawa T, Ohashi T, Eto Y, Yanaga K.  Adenovirus-mediated CD40L gene therapy induced both humoral and cellular immunity against rat model of hepatocellular carcinoma.  Cancer Sci.  2008  99:2097-103  PubMed ID: 19016771   DOI: 10.1111/j.1349-7006.2008.00953.x
4713  Taguchi E, Nakamura K, Miura T, Shibuya M, Isoe T.  Anti-tumor activity and tumor vessel normalization by the vascular endothelial growth factor receptor tyrosine kinase inhibitor KRN951 in a rat peritoneal disseminated tumor model.  Cancer Sci  2008  99(3):623-30  PubMed ID: 18201272   DOI: 10.1111/j.1349-7006.2007.00724.x
4714  Sakurai M, Misawa T, Shiba H, Iida T, Ohashi T, Yanaga K.  A novel approach for gene transduction with adenovirus vector and the fibrin glue system.  Anticancer Res  2008  28(6A):3809-13  PubMed ID: 19189668  
4720  Hanyu K, Iida T, Shiba H, Ohashi T, Eto Y, Yanaga K.  Immunogene therapy by adenovirus vector expressing CD40 ligand for metastatic liver cancer in rats.  Anticancer Res  2008  28(5A):2785-9  PubMed ID: 19035311  
4721  Mutoh M, Niho N, Komiya M, Takahashi M, Ohtsubo R, Nakatogawa K, Ueda K, Sugimura T, Wakabayashi K.  Plasminogen activator inhibitor-1 (Pai-1) blockers suppress intestinal polyp formation in Min mice.  Carcinogenesis  2008  29(4):824-9  PubMed ID: 18258607   DOI: 10.1093/carcin/bgn028
4726  Tamagawa K1, Horiuchi T, Uchinami M, Doi K, Yoshida M, Nakamura T, Sasaki H, Taniguchi M, Tanaka K.  Hepatic ischemia-reperfusion increases vascular endothelial growth factor and cancer growth in rats.  J Surg Res  2008  148(2):158-63  PubMed ID: 18468635   DOI: 10.1016/j.jss.2007.12.787
4731  Teye K, Okamoto K, Tanaka Y, Umata T, Ohnuma M, Moroi M, Kimura H, Tsuneoka M.  Expression of the TAF4b gene is induced by MYC through a non-canonical, but not canonical, E-box which contributes to its specific response to MYC.  Int J Oncol  2008  33(6):1271-80  PubMed ID: 19020761  
4722  Iguchi K, Oh G, Ookawa K, Yanagi K, Sakai M, Yamamoto T, Ishikawa S, Onizuka M.  In vivo observation of pulmonary micrometastasis of colon cancer in normal rats.  Microvasc Res  2007  73(3):206-13  PubMed ID: 17382972   DOI: 10.1016/j.mvr.2007.02.003
4724  Hamaguchi T, Matsuoka Y, Bechberger J, Ohnishi T, Fujita K, Naus CC, Kusunoki M, Tsubura A, Tsuda H.  Establishment of an apoptosis-sensitive rat mammary carcinoma cell line with a mutation in the DNA-binding region of p53.  Cancer Lett  2006  232(2):279-88  PubMed ID: 15876486   DOI: 10.1016/j.canlet.2005.02.043
17379  Ohsawa I, Murakami T, Uemoto S, Kobayashi E.  In vivo luminescent imaging of cyclosporin A-mediated cancer progression in rats  Transplantation  2006  81(11):1558-67  PubMed ID: 16770245   DOI: 10.1097/01.tp.0000209448.50238.de
3029  Kobayashi H, Uetake H, Higuchi T, Enomoto M, Sugihara K  JTE-522, a selective COX-2 inhibitor, inhibits growth of pulmonary metastases of colorectal cancer in rats.  BMC Cancer  2005  5:26  PubMed ID: 15743541   DOI: 10.1186/1471-2407-5-26
4712  Sako T, Nakayama Y, Minagawa N, Inoue Y, Onitsuka K, Katsuki T, Tsurudome Y, Shibao K, Hirata K, Nagata N, Ohie S, Kohno K, Itoh H.  4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid (TAC-101) induces apoptosis in colon cancer partially through the induction of Fas expression.  In Vivo  2005  19(1):125-32  PubMed ID: 15796164  
2688  Takahashi, Tetsuyuki, Takasuka, Nobuo, Iigo, Masaaki, Baba, Masaki, Nishino, Hoyoku, Tsuda, Hiroyuki, Okuyama, Toru  Isoliquiritigenin, a flavonoid from licorice, reduces prostaglandin E2 and nitric oxide, causes apoptosis, and suppresses aberrant crypt foci development.  Cancer Sci  2004  95:448-53  PubMed ID: 15132774   DOI: 10.1111/j.1349-7006.2004.tb03230.x
3030  Kobayashi H, Gonda T, Uetake H, Higuchi T, Enomoto M, Sugihara K.  JTE-522, a selective COX-2 inhibitor, interferes with the growth of lung metastases from colorectal cancer in rats due to inhibition of neovascularization: a vascular cast model study.  Int. J. Cancer  2004  112:920-6  PubMed ID: 15386343   DOI: 10.1002/ijc.20523
4734  Ishikawa E1, Tsuboi K, Takano S, Uchimura E, Nose T, Ohno T.  Intratumoral injection of IL-2-activated NK cells enhances the antitumor effect of intradermally injected paraformaldehyde-fixed tumor vaccine in a rat intracranial brain tumor model.  Cancer Sci  2004  95(1):98-103  PubMed ID: 14720334   DOI: 10.1111/j.1349-7006.2004.tb03177.x
4732  Takahashi M, Mutoh M, Shoji Y, Kamanaka Y, Naka M, Maruyama T, Sugimura T, Wakabayashi K.  Transfection of K-rasAsp12 cDNA markedly elevates IL-1beta- and lipopolysaccharide-mediated inducible nitric oxide synthase expression in rat intestinal epithelial cells.  Oncogene  2003  22(48):7667-76  PubMed ID: 14576830   DOI: 10.1038/sj.onc.1207051
4733  Miyoshi C, Taguchi E, Ohshima N.  Leukocyte behavior in angiogenic vessels is affected by tumor-derived nitric oxide.  Jpn J Physiol  2003  53(5):343-50  PubMed ID: 14975180   DOI: 10.2170/jjphysiol.53.343
4710  Karube H1, Masuda H, Ishii Y, Takayama T.  E-cadherin expression is inversely proportional to tumor size in experimental liver metastases.  J Surg Res  2002  106(1):173-8  PubMed ID: 12127823   DOI: 10.1006/jsre.2002.6447
4711  Tanji H, Yahata H, Hayamizu K, Shinozaki K, Okimoto T, Sakaguchi T, Asahara T.  Augmentation of local antitumor immunity in liver by interleukin-2 gene transfer via portal vein.  Cancer Gene Ther  2002  9(8):655-64  PubMed ID: 12136426   DOI: 10.1038/sj.cgt.7700483
4717  Doi K, Horiuchi T, Uchinami M, Tabo T, Kimura N, Yokomachi J, Yoshida M, Tanaka K.  Neutrophil elastase inhibitor reduces hepatic metastases induced by ischaemia-reperfusion in rats.  Eur J Surg  2002  168(8-9):507-10  PubMed ID: 12549694   DOI: 10.1080/110241502321116541
4719  Doi K, Horiuchi T, Uchinami M, Tabo T, Kimura N, Yokomachi J, Yoshida M, Tanaka K.  Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer.  J Surg Res  2002  105(2):243-7  PubMed ID: 12121713   DOI: 10.1006/jsre.2002.6356
4728  Tsuneoka M, Koda Y, Soejima M, Teye K, Kimura H.  A novel myc target gene, mina53, that is involved in cell proliferation.  J Biol Chem  2002  277(38):35450-9  PubMed ID: 12091391   DOI: 10.1074/jbc.M204458200
4718  Ohkame H, Masuda H, Ishii Y, Kanai Y.  Expression of L-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in liver tumor lesions of rat models.  J Surg Oncol  2001  78(4):265-71  PubMed ID: 11745822   DOI: 10.1002/jso.1165
17467  Yang W, Arii S, Mori A, Furumoto K, Nakao T, Isobe N, Murata T, Onodera H, Imamura M.  sFlt-1 gene-transfected fibroblasts: a wound-specific gene therapy inhibits local cancer recurrence  Cancer Res  2001  61(21):7840-5  PubMed ID: 11691801  
4709  Soejima M, Koda Y, Wang B, Kimura H.  Functional analysis of the 5'-flanking region of FTA for expression of rat GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase.  Eur J Biochem  1999  266(1):274-81  PubMed ID: 10542075   DOI: 10.1046/j.1432-1327.1999.00865.x
4727  Suzuki T, Yanagi K, Ookawa K, Hatakeyama K, Ohshima N.  Blood flow and leukocyte adhesiveness are reduced in the microcirculation of a peritoneal disseminated colon carcinoma.  Ann Biomed Eng  1998  26(5):803-11  PubMed ID: 9779953   DOI: 10.1114/1.67
4708  Ohsawa K1, Watanabe Y, Takakura A, Itoh T, Sato H.  Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.  Exp Anim  1996  45(4):389-93  PubMed ID: 8902504   DOI: 10.1538/expanim.45.389
4716  Yanagi K, Ohshima N.  Angiogenic vascular growth in the rat peritoneal disseminated tumor model.  Microvasc Res  1996  51(1):15-28  PubMed ID: 8812750   DOI: 10.1006/mvre.1996.0003

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利用者成果(日)
4730  張 仁俊  大腸癌術後のimplantationによる器械吻合部における局所再発とpovidone-iodine液によるその予防に関する実験的研究  日本消化器外科学会雑誌  1997  30:1847-1855    DOI: 10.5833/jjgs.30.1847
2185  陣内浩喜、奥野清隆  消化器癌肝転移における癌由来免疫抑制物質prostaglandin E_2の影響  近畿大学医学雑誌  1996  21(1):49-63   



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