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Cell No. : Cell Name
RCB0510 : ACL-15  update : 2023/11/30
CommentMetastasis to liver, lung
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kashima, Yuji
Originator Kashima, Yuji
Year of deposit 1990
Animal _rat < Mammals
Strain name F344
Genus Rattus
Species norvegicus
Gender Male
Tissue colon
Classification cancer
Year of origin 1988
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5491
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, MPI
Chromosome mode 88-117(50) : 88(6),89(29),90(10),91(4),117(1)
deposit info
lot info
Reference information Reference 3
User's Publication 5

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4707  Ohkawa A1, Sato N, Hatakeyama K.  Effect of medium-chain triglyceride emulsion in total parenteral nutrition on experimental hepatic metastasis in the rat.  JPEN J Parenter Enteral Nutr  1997  21(4):220-3  PubMed ID: 9252948   DOI: 10.1177/0148607197021004220
4706  Sugimoto F, Tsukada K, Hatakeyama K, Yoshida K, Muto T.  OK432 inhibits experimental hepatic metastasis of colon adenocarcinoma ACL-15 in F344 rats.  Surg Today  1994  24(8):694-700  PubMed ID: 7981540   DOI: 10.1007/bf01636774
1369  Kashima, Yuji  The Establishment of a Rat Colon Cancer Cell Line, ACL-15, with an Analysis of Its Metastatic Properties  Acta Medica et Biologica  1991  39:75-81   

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User's Publication
8675  Jin Y, Takegahara Y, Sugawara Y, Matsumura T, Fujinaga Y.  Disruption of the epithelial barrier by botulinum haemagglutinin (HA) proteins - differences in cell tropism and the mechanism of action between HA proteins of types A or B, and HA proteins of type C.  Microbiology (Reading, Engl.)  2009  155:35-45  PubMed ID: 19118344   DOI: 10.1099/mic.0.021246-0
15613  Kato T, Tsuchida T, Kawamoto K.  Blood tumor permeability of experimental brain tumor: an electron microscopic study using lanthanum  Neuropathology  2005  25(1):21-6  PubMed ID: 15822815   DOI: 10.1111/j.1440-1789.2004.00575.x
19091  Tohoru Takeda, Akio Yoneyama, Jin Wu, Thet Thet Lwin, Yoshinori Tsuchiya, Kazuyuki Hyodo  In-Vivo Imaging of Cancer Implanted in Nude Mice by Two-Crystal Interferometer-Based Phase-Contrast X-Ray Computed Tomography  Japanese Journal of Applied Physics  2004  43:L1144    DOI: 10.1143/jjap.43.l1144
17466  Momen MA, Monden Y, Houchi H, Umemoto A.  Effect of ursodeoxycholic acid on azoxymethane-induced aberrant crypt foci formation in rat colon: in vitro potential role of intracellular Ca2+  J Med Invest  2002  49(1-2):67-73  PubMed ID: 11901763  
3687  Masunaga R, Kohno H, Kumar Dhar D, Kotoh T, Tachibana M, Kubota H, Nagasue N.  Sulindac inhibits growth of rat colon carcinoma by inducing apoptosis  Eur Surg Res  2000  32(5):305-9  PubMed ID: 11111176   DOI: 10.1159/000008780

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