| Cell No. : Cell Name RCB0510 : ACL-15
						
						 update : 2023/11/30
 | 
						
						
						
							
								| Comment | Metastasis to liver, lung | 
							
								| Comment from the depositor |  | 
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
								| Remarks |  | 
							
							
								| Order Form | Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										  | 
							
								| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | 
						
						
					
						
						
							
							|  | Basic information | Depositor | Kashima, Yuji | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Originator | Kashima, Yuji | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 1990 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | _rat
								
								
								
							
								 
								
								
								 	
								 		< Mammals | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Strain name | F344 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Genus | Rattus | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Species | norvegicus | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Gender | Male | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Tissue | colon | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | cancer | 
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of origin | 1988 | 
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							 | Lifespan | infinite | 
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | epithelial-like | 
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | CVCL_5491 | 
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								|  |  | deposit info | lot info | 
											
												|  | Medium | Medium List | 
										
									
								
						
						
						
						
						
					
						
						
						
						
							 | Culture type |  | Adherent cells | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Culture medium |  | RPMI1640 + 10% FBS | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Antibiotics |  | Free | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							 | Passage method |  | 0.25% Trypsin | Culture information | Passage ratio |  | 1 : 8 split | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| SC frequency |  | Subculture : 2 times/week | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Temperature |  | 37
							
							
						
						
							℃ | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| CO2 concentration |  | 5
							
							
						
						
							% | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freeze medium |  | Medium + 10% DMSO | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method |  | Slow freezing | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Mycoplasma/Acholeplasma |  | (-) | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Animal PCR |  | OK | 
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Isozyme |  | LD, NP, AST, MPI | 
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Chromosome mode |  | 88-117(50) : 88(6),89(29),90(10),91(4),117(1) | 
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
						
							
							
							
								| Images | deposit info | lot info | 
							
							
							|  |  | 
					
			
					
			
						
						
						
							
								| Reference information | Reference | 3 | 
							
								| User's Publication | 6 | 
						
						
				
			
				
				
				
				
				
					
						| Reference | 
						
							
							| 4707 
								Ohkawa A1, Sato N, Hatakeyama K. 
								Effect of medium-chain triglyceride emulsion in total parenteral nutrition on experimental hepatic metastasis in the rat. 
								JPEN J Parenter Enteral Nutr 
								1997 
								21(4):220-3 
								
									
										PubMed ID: 9252948
									
								 
								
									
										DOI: 10.1177/0148607197021004220 | 
						
							
							| 4706 
								Sugimoto F, Tsukada K, Hatakeyama K, Yoshida K, Muto T. 
								OK432 inhibits experimental hepatic metastasis of colon adenocarcinoma ACL-15 in F344 rats. 
								Surg Today 
								1994 
								24(8):694-700 
								
									
										PubMed ID: 7981540
									
								 
								
									
										DOI: 10.1007/bf01636774 | 
						
							
							| 1369 
								Kashima, Yuji 
								The Establishment of a Rat Colon Cancer Cell Line, ACL-15, with an Analysis of Its Metastatic Properties 
								Acta Medica et Biologica 
								1991 
								39:75-81 | 
						
					
				
					
						| User's Publication | 
						
							
							| 8675 
								Jin Y, Takegahara Y, Sugawara Y, Matsumura T, Fujinaga Y. 
								Disruption of the epithelial barrier by botulinum haemagglutinin (HA) proteins - differences in cell tropism and the mechanism of action between HA proteins of types A or B, and HA proteins of type C. 
								Microbiology (Reading, Engl.) 
								2009 
								155:35-45 
								
									
										PubMed ID: 19118344
									
								 
								
									
										DOI: 10.1099/mic.0.021246-0 | 
						
							
							| 15613 
								Kato T, Tsuchida T, Kawamoto K. 
								Blood tumor permeability of experimental brain tumor: an electron microscopic study using lanthanum 
								Neuropathology 
								2005 
								25(1):21-6 
								
									
										PubMed ID: 15822815
									
								 
								
									
										DOI: 10.1111/j.1440-1789.2004.00575.x | 
						
							
							| 19091 
								Tohoru Takeda, Akio Yoneyama, Jin Wu, Thet Thet Lwin, Yoshinori Tsuchiya, Kazuyuki Hyodo 
								In-Vivo Imaging of Cancer Implanted in Nude Mice by Two-Crystal Interferometer-Based Phase-Contrast X-Ray Computed Tomography 
								Japanese Journal of Applied Physics 
								2004 
								43:L1144 
								 
								
									
										DOI: 10.1143/jjap.43.l1144 | 
						
							
							| 17466 
								Momen MA, Monden Y, Houchi H, Umemoto A. 
								Effect of ursodeoxycholic acid on azoxymethane-induced aberrant crypt foci formation in rat colon: in vitro potential role of intracellular Ca2+ 
								J Med Invest 
								2002 
								49(1-2):67-73 
								
									
										PubMed ID: 11901763 | 
						
							
							| 3687 
								Masunaga R, Kohno H, Kumar Dhar D, Kotoh T, Tachibana M, Kubota H, Nagasue N. 
								Sulindac inhibits growth of rat colon carcinoma by inducing apoptosis 
								Eur Surg Res 
								2000 
								32(5):305-9 
								
									
										PubMed ID: 11111176
									
								 
								
									
										DOI: 10.1159/000008780 | 
						
							
							| 1400 
								Iwamoto S, Senzaki H, Kiyozuka Y, Ogura E, Takada H, Hioki K, Tsubura A. 
								Effects of fatty acids on liver metastasis of ACL-15 rat colon cancer cells 
								Nutr Cancer 
								1998 
								31(2):143-50 
								
									
										PubMed ID:  9770727
									
								 
								
									
										DOI: 10.1080/01635589809514694 |