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Cell No. : Cell Name
RCB0508 : H-EMC-SS  update : 2022/03/16
CommentExtraskeletal myxoid chondrosarcoma
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Saotome, Kouichi
Originator Saotome, K. & Sibukawa, A.
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 77 years
Tissue muscle
Disease name chondrosarcoma
Metastatic ability Yes
Metastatic tissue lymph node,lung
Classification cancer
Year of origin 1987
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1238
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA (or DM-160AU) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 45-47(50) : 45(5),46(43),47(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


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Reference
1727  Oohima,Fumio  Establishment of A New Cell Line from Human Extraskeletal Myxoid Chondrosarcoma  Dokkyo Journal of Medical Sciences  1992  19:43109 

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User's Publication
10779  Goto H, Nishio M, To Y, Oishi T, Miyachi Y, Maehama T, Nishina H, Akiyama H, Mak TW, Makii Y, Saito T, Yasoda A, Tsumaki N, Suzuki A.  Loss of Mob1a/b in mice results in chondrodysplasia due to YAP1/TAZ-TEAD-dependent repression of SOX9.  Development  2018  145:  PubMed ID: 29511023
7810  Akiyama K, Tone J, Yamauchi S, Sugahara T, Maruyama M, Kakinuma Y.  Disruption of ion homeostasis by verrucosin and a related compound.  Biosci. Biotechnol. Biochem.  2011  75:1000-2  PubMed ID: 21597167
6475  Osaki M, Osaki M, Yamashita H, Shomori K, Yoshida H, Ito H.  Expression of minichromosome maintenance-2 in human malignant fibrous histiocytomas: Correlations with Ki-67 and P53 expression, and apoptosis.  Int J Mol Med  2002  10(2):161-8  PubMed ID: 12119552
1902  Shu Qin Liu, Kaoru Saijo, Makoto Kodama, Tadao Ohno  Intelligent matrices for tumor-specific cytotoxic T lymphocytes  Materials Science and Engineering: C  1994  2:61-65 



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