Cell No. : Cell Name
RCB0505 : MR6
update : 2022/03/16
|
| Comment | MNNG-resistant HeLa.S3(Mer-) cells |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishida, Ryoji
|
| Originator |
Ishida, Ryoji
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_U183
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 2% FBS + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP, AST, MPI
|
| Chromosome mode |
|
57-61(50) : 57(2),58(4),59(16),60(26),61(2)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
|
| User's Publication |
1
|
| Reference |
|
4702
Ishida R1, Utsumi KR.
Differences in induction of sister chromatid exchanges in MNNG-resistant HeLa S3 Mer- cell lines.
Carcinogenesis
1990
11(7):1209-12
PubMed ID: 2372878
DOI: 10.1093/carcin/11.7.1209
|
|
1162
Ishida R, Utsumi, K R, Takahashi T
Sister-chromatid exchanges (SCEs), cell survival and mutation in HeLa s3 cells with different sensitivity to alkylating agents; evidence that SCE induction and cell survival or mutation induction are dissociable.
Mutat Res
1989
215:69-77
PubMed ID: 2811915
DOI: 10.1016/0027-5107(89)90219-4
|
|
1164
Ishida R, Takahashi T
Increased O6-methylguanine-DNA methyltransferase activity and reduced mutability in 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2- chloroethyl)-3-nitrosourea-resistant HeLa S3 cells.
Carcinogenesis
1988
9:1079-83
PubMed ID: 3163531
DOI: 10.1093/carcin/9.6.1079
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|
1163
Ishida R, Takahashi T
N-methyl-N'-nitro-N-nitrosoguanidine-resistant HeLa S3 cells still have little O6-methylguanine-DNA methyltransferase activity and are hypermutable by alkylating agents.
Carcinogenesis
1987
8:1109-13
PubMed ID: 3608093
DOI: 10.1093/carcin/8.8.1109
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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