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Cell No. : Cell Name
RCB0504 : MR1-3  update : 2022/03/16
CommentMNNG-reistant HeLa.S3(Mer-) cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishida, Ryoji
Originator Ishida, Ryoji
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U180
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 2% FBS + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 58-63(50) : 58(1),59(3),60(10),61(20),62(11),63(5)
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 1

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4702  Ishida R1, Utsumi KR.  Differences in induction of sister chromatid exchanges in MNNG-resistant HeLa S3 Mer- cell lines.  Carcinogenesis  1990  11(7):1209-12  PubMed ID: 2372878   DOI: 10.1093/carcin/11.7.1209
1162  Ishida R, Utsumi, K R, Takahashi T  Sister-chromatid exchanges (SCEs), cell survival and mutation in HeLa s3 cells with different sensitivity to alkylating agents; evidence that SCE induction and cell survival or mutation induction are dissociable.  Mutat Res  1989  215:69-77  PubMed ID: 2811915   DOI: 10.1016/0027-5107(89)90219-4
1164  Ishida R, Takahashi T  Increased O6-methylguanine-DNA methyltransferase activity and reduced mutability in 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2- chloroethyl)-3-nitrosourea-resistant HeLa S3 cells.  Carcinogenesis  1988  9:1079-83  PubMed ID: 3163531   DOI: 10.1093/carcin/9.6.1079
1163  Ishida R, Takahashi T  N-methyl-N'-nitro-N-nitrosoguanidine-resistant HeLa S3 cells still have little O6-methylguanine-DNA methyltransferase activity and are hypermutable by alkylating agents.  Carcinogenesis  1987  8:1109-13  PubMed ID: 3608093   DOI: 10.1093/carcin/8.8.1109
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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