Cell No. : Cell Name
RCB0503 : HeLa.S3(Mer^(-))
update : 2023/06/30
|
| Comment | Repair deficient (mer-) |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishida, Ryoji
|
| Originator |
Gey, G. O. et al
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_U209
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 2% FBS + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP, AST, MPI
|
| Chromosome mode |
|
58-63(50) : 58(1),59(3),60(10),61(20),62(11),63(5)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
11
|
| User's Publication |
1
|
| Reference |
|
4702
Ishida R1, Utsumi KR.
Differences in induction of sister chromatid exchanges in MNNG-resistant HeLa S3 Mer- cell lines.
Carcinogenesis
1990
11(7):1209-12
PubMed ID: 2372878
DOI: 10.1093/carcin/11.7.1209
|
|
1162
Ishida R, Utsumi, K R, Takahashi T
Sister-chromatid exchanges (SCEs), cell survival and mutation in HeLa s3 cells with different sensitivity to alkylating agents; evidence that SCE induction and cell survival or mutation induction are dissociable.
Mutat Res
1989
215:69-77
PubMed ID: 2811915
DOI: 10.1016/0027-5107(89)90219-4
|
|
1164
Ishida R, Takahashi T
Increased O6-methylguanine-DNA methyltransferase activity and reduced mutability in 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2- chloroethyl)-3-nitrosourea-resistant HeLa S3 cells.
Carcinogenesis
1988
9:1079-83
PubMed ID: 3163531
DOI: 10.1093/carcin/9.6.1079
|
|
1163
Ishida R, Takahashi T
N-methyl-N'-nitro-N-nitrosoguanidine-resistant HeLa S3 cells still have little O6-methylguanine-DNA methyltransferase activity and are hypermutable by alkylating agents.
Carcinogenesis
1987
8:1109-13
PubMed ID: 3608093
DOI: 10.1093/carcin/8.8.1109
|
|
4703
Day RS 3rd, Yarosh DB, Ziolkowski CH.
Relationship of methyl purines produced by MNNG in adenovirus 5 DNA to viral inactivation in repair-deficient (Mer-) human tumor cell strains.
Mutat Res
1984
131(2):45-52
PubMed ID: 6700617
DOI: 10.1016/0167-8817(84)90010-5
|
|
4704
Yarosh DB, Foote RS, Mitra S, Day RS 3rd.
Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.
Carcinogenesis
1983
4(2):199-205
PubMed ID: 6825208
DOI: 10.1093/carcin/4.2.199
|
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4705
Foote RS, Pal BC, Mitra S.
Quantitation of O6-methylguanine-DNA methyltransferase in HeLa cells.
Mutat Res
1983
119(3):221-8
PubMed ID: 6828059
DOI: 10.1016/0165-7992(83)90164-1
|
|
350
Kuroki T.
Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Exp Cell Res
1973
80(1):55-62
PubMed ID: 4361348
DOI: 10.1016/0014-4827(73)90274-7
|
|
1446
J E DARNELL Jr, T K SAWYERc
Variation in plaque-forming ability among parental and clonal strains of HeLa cells
Virology
1959
223-9
PubMed ID: 13669339
DOI: 10.1016/0042-6822(59)90006-6
|
|
206
PUCK TT, MARCUS PI, CIECIURA SJ.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J Exp Med
1956
103(2):273-83
PubMed ID: 13286432
DOI: 10.1084/jem.103.2.273
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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