Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0503 : HeLa.S3(Mer^(-))  update : 2022/03/16
CommentRepair deficient (mer-)
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishida, Ryoji
Originator Gey, G. O. et al
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U209
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 2% FBS + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, AST, MPI
Chromosome mode 58-63(50) : 58(1),59(3),60(10),61(20),62(11),63(5)
STR(human) OK
Reference information Reference 10
User's Publication 1


To topTop
Reference
4702  Ishida R1, Utsumi KR.  Differences in induction of sister chromatid exchanges in MNNG-resistant HeLa S3 Mer- cell lines.  Carcinogenesis  1990  11(7):1209-12  PubMed ID: 2372878   DOI: 10.1093/carcin/11.7.1209
1162  Ishida R, Utsumi, K R, Takahashi T  Sister-chromatid exchanges (SCEs), cell survival and mutation in HeLa s3 cells with different sensitivity to alkylating agents; evidence that SCE induction and cell survival or mutation induction are dissociable.  Mutat Res  1989  215:69-77  PubMed ID: 2811915  
1164  Ishida R, Takahashi T  Increased O6-methylguanine-DNA methyltransferase activity and reduced mutability in 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2- chloroethyl)-3-nitrosourea-resistant HeLa S3 cells.  Carcinogenesis  1988  9:1079-83  PubMed ID: 3163531  
1163  Ishida R, Takahashi T  N-methyl-N'-nitro-N-nitrosoguanidine-resistant HeLa S3 cells still have little O6-methylguanine-DNA methyltransferase activity and are hypermutable by alkylating agents.  Carcinogenesis  1987  8:1109-13  PubMed ID: 3608093  
4703  Day RS 3rd, Yarosh DB, Ziolkowski CH.  Relationship of methyl purines produced by MNNG in adenovirus 5 DNA to viral inactivation in repair-deficient (Mer-) human tumor cell strains.  Mutat Res  1984  131(2):45-52  PubMed ID: 6700617   DOI: 10.1016/0167-8817(84)90010-5
4704  Yarosh DB, Foote RS, Mitra S, Day RS 3rd.  Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.  Carcinogenesis  1983  4(2):199-205  PubMed ID: 6825208   DOI: 10.1093/carcin/4.2.199
4705  Foote RS, Pal BC, Mitra S.  Quantitation of O6-methylguanine-DNA methyltransferase in HeLa cells.  Mutat Res  1983  119(3):221-8  PubMed ID: 6828059   DOI: 10.1016/0165-7992(83)90164-1
350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348   DOI: 10.1016/0014-4827(73)90274-7
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432   DOI: 10.1084/jem.103.2.273
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

To topTop
User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x



Back Back Return Top Page