細胞番号 : 細胞名
RCB0497 : KU812F
update : 2024/01/17
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| 細胞特性(Comment:英) | Chronic myelogeneous leukemia, subclone of RCB0495 KU812. |
| 細胞特性(日) | 幼若好塩基球と考えられる慢性骨髄性白血病細胞。赤芽球への分化も見られる。RCB0495 KU812のクローン。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Mizusawa, Hiroshi
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| 樹立者 |
Kishi, Kenji
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| 寄託日 |
1990
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Male
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| 採取組織 |
blood
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| 細胞分類 |
cancer
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| 入手歴 |
JCRB0104.2
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細胞寿命 |
infinite
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| 細胞形態 |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_0616
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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| 培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| 培地 |
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RPMI1640 + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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dilution
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継代密度 |
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1 : 10 split
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| 継代・培地交換頻度 |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| ウィルス（HIV) |
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(-)
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| ウィルス（HTLV-1) |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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55-64(50) : /59(5),60(7),61(10),62(20),63(2),64(1)
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| 個体識別検査 |
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OK
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| 文献情報 |
Reference(英) |
14件
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| Reference(日) |
1件
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| 利用者成果(英) |
4件
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| 利用者成果(日) |
0件
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| Reference(英) |
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1777
Masuko M, Koike T, Toba K, Kishi K, Kuroha T, Furukawa T, Fuse I, Takahashi M, Kuwano R, Shibata A, Aizawa Y.
Expression of eosinophil peroxidase in the immature basophil cell line KU812-F.
Leuk Res
1999
23(2):99-104
PubMed ID: 10071125
DOI: 10.1016/s0145-2126(98)00140-4
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21
Fukuda T, Kamishima T, Tsuura Y, Suzuki T, Kakihara T, Naito M, Kishi K, Matsumoto K, Shibata A, Seito T.
Expression of the c-kit gene product in normal and neoplastic mast cells but not in neoplastic basophil/mast cell precursors from chronic myelogenous leukaemia
J Pathol
1995
177(2):139-46
PubMed ID: 7490680
DOI: 10.1002/path.1711770207
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26
Fukuda T, Kakihara T, Kamishima T, Ohnishi Y, Naito M, Kishi K, Shibata A.
Leukemic cell membrane from acute myelogenous leukemias with massive mast cell infiltration has a mast cell differentiation activity under culture condition containing interleukin 3
Leuk Res
1994
18(10):749-59
PubMed ID: 7934132
DOI: 10.1016/0145-2126(94)90056-6
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23
Fukuda T, Kakihara T, Ohnishi Y, Sato K, Hasegawa T, Momozaki S, Kishi K, Shibata A, Abe A, Kurokawa I.
Abnormal granulopoiesis of leukemic cells with basophil/mast cell features. Cytochemical and ultrastructural observations
Acta Pathol Jpn
1991
41(7):531-9
PubMed ID: 1755319
DOI: 10.1111/j.1440-1827.1991.tb02518.x
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24
Fukuda T, Kakihara T, Ohnishi Y, Kishi K, Shibata A, Narita M, Kurokawa I, Abe A, Shimizu S.
Heterogeneity of Leukemic Cells with Basophilic Features: Cytochemical, Ultrastructural and Immunophonotypic Analysis of 8 Cases
Leuk Lymphoma
1991
5(5-6):371-8
PubMed ID: 27463348
DOI: 10.3109/10428199109067631
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1796
Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.
Failure to detect IL-3-binding sites on human mast cells.
J Immunol
1990
145:3432-7
PubMed ID: 2230127
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256
Valent P, Besemer J, Kishi K, Kaltenbrunner R, Kuhn B, Maurer D, Lechner K, Bettelheim P.
IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites
J Immunol
1990
145(6):1885-9
PubMed ID: 2144011
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1776
Valent P, Besemer J, Kishi K, Di Padova F, Geissler K, Lechner K, Bettelheim P.
Human basophils express interleukin-4 receptors.
Blood
1990
76(9):1734-8
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22
Mamune-Sato R, Tanno Y, Maeyama K, Miura Y, Takishima T, Kishi K, Fukuda T, Watanabe T.
Histidine decarboxylase in human basophilic leukemia (KU-812-F) cells. Characterization and induction by phorbol myristate acetate
Biochem Pharmacol
1990
40(5):1125-9
PubMed ID: 2117926
DOI: 10.1016/0006-2952(90)90502-c
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1795
Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.
KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.
Blood
1989
73:2003-13
PubMed ID: 2540861
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1774
Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.
Induction of differentiation of the human leukemia cell line, KU812.
Leuk Res
1987
11(12):1105-13
PubMed ID: 3695564
DOI: 10.1016/0145-2126(87)90164-0
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1765
Fukuda T, Kishi K, Ohnishi Y, Shibata A.
Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.
Blood
1987
70(3):612-9
PubMed ID: 3304457
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1785
Kishi K.
A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Leuk Res
1985
9(3):381-90
PubMed ID: 3858609
DOI: 10.1016/0145-2126(85)90060-8
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1784
Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.
A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia
Nihon Ketsueki Gakkai Zasshi
1984
47(3):709-18
PubMed ID: 6591673
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| 利用者成果(英) |
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14327
Caillet-Fauquet P, Draps ML, Di Giambattista M, de Launoit Y, Laub R.
Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line
J Virol Methods
2004
121(2):145-53
PubMed ID: 15381351
DOI: 10.1016/j.jviromet.2004.06.010
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14328
Caillet-Fauquet P, Di Giambattista M, Draps ML, Sandras F, Branckaert T, de Launoit Y, Laub R.
Continuous-flow UVC irradiation: a new, effective, protein activity-preserving system for inactivating bacteria and viruses, including erythrovirus B19
J Virol Methods
2004
118(2):131-9
PubMed ID: 15081608
DOI: 10.1016/j.jviromet.2004.02.002
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15565
Makoto Fujii, Yasushi Tanimoto, Minoru Takata, Kazushi Takao, Noboru Hamada, Toshimitsu Suwaki, Noriko Kawata, Kiyoshi Takahashi, Mine Harada, Mitsune Tanimoto
Association of IgG Fc receptor II with tyrosine kinases in the human basophilic leukemia cell line KU812F
Allergology International
2003
52:149-154
DOI: 10.1046/j.1440-1592.2003.00291.x
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2050
Kawata N, Takata M, Suwaki T, Tanimoto Y, Soda R, Takahashi K, Kimura I
Signal transduction by IgG receptors induces calcium mobilization, but not histamine release, in the human basophilic cell line KU812F.
Int Arch Allergy Immunol
1996
109:27-34
PubMed ID: 8527947
DOI: 10.1159/000237228
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