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Cell No. : Cell Name
RCB0497 : KU812F  update : 2024/01/17
CommentChronic myelogeneous leukemia, subclone of RCB0495 KU812.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mizusawa, Hiroshi
Originator Kishi, Kenji
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Classification cancer
History JCRB0104.2
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0616
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 55-64(50) : /59(5),60(7),61(10),62(20),63(2),64(1)
STR(human) OK
Reference information Reference 14
User's Publication 4

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1777  Masuko M, Koike T, Toba K, Kishi K, Kuroha T, Furukawa T, Fuse I, Takahashi M, Kuwano R, Shibata A, Aizawa Y.  Expression of eosinophil peroxidase in the immature basophil cell line KU812-F.  Leuk Res  1999  23(2):99-104  PubMed ID: 10071125   DOI: 10.1016/s0145-2126(98)00140-4
21  Fukuda T, Kamishima T, Tsuura Y, Suzuki T, Kakihara T, Naito M, Kishi K, Matsumoto K, Shibata A, Seito T.  Expression of the c-kit gene product in normal and neoplastic mast cells but not in neoplastic basophil/mast cell precursors from chronic myelogenous leukaemia  J Pathol  1995  177(2):139-46  PubMed ID: 7490680   DOI: 10.1002/path.1711770207
26  Fukuda T, Kakihara T, Kamishima T, Ohnishi Y, Naito M, Kishi K, Shibata A.  Leukemic cell membrane from acute myelogenous leukemias with massive mast cell infiltration has a mast cell differentiation activity under culture condition containing interleukin 3  Leuk Res  1994  18(10):749-59  PubMed ID: 7934132   DOI: 10.1016/0145-2126(94)90056-6
23  Fukuda T, Kakihara T, Ohnishi Y, Sato K, Hasegawa T, Momozaki S, Kishi K, Shibata A, Abe A, Kurokawa I.  Abnormal granulopoiesis of leukemic cells with basophil/mast cell features. Cytochemical and ultrastructural observations  Acta Pathol Jpn  1991  41(7):531-9  PubMed ID: 1755319   DOI: 10.1111/j.1440-1827.1991.tb02518.x
24  Fukuda T, Kakihara T, Ohnishi Y, Kishi K, Shibata A, Narita M, Kurokawa I, Abe A, Shimizu S.  Heterogeneity of Leukemic Cells with Basophilic Features: Cytochemical, Ultrastructural and Immunophonotypic Analysis of 8 Cases  Leuk Lymphoma  1991  5(5-6):371-8  PubMed ID: 27463348   DOI: 10.3109/10428199109067631
1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127  
256  Valent P, Besemer J, Kishi K, Kaltenbrunner R, Kuhn B, Maurer D, Lechner K, Bettelheim P.  IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites  J Immunol  1990  145(6):1885-9  PubMed ID: 2144011  
1776  Valent P, Besemer J, Kishi K, Di Padova F, Geissler K, Lechner K, Bettelheim P.  Human basophils express interleukin-4 receptors.  Blood  1990  76(9):1734-8   
22  Mamune-Sato R, Tanno Y, Maeyama K, Miura Y, Takishima T, Kishi K, Fukuda T, Watanabe T.  Histidine decarboxylase in human basophilic leukemia (KU-812-F) cells. Characterization and induction by phorbol myristate acetate  Biochem Pharmacol  1990  40(5):1125-9  PubMed ID: 2117926   DOI: 10.1016/0006-2952(90)90502-c
1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861  
1774  Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.  Induction of differentiation of the human leukemia cell line, KU812.  Leuk Res  1987  11(12):1105-13  PubMed ID: 3695564   DOI: 10.1016/0145-2126(87)90164-0
1765  Fukuda T, Kishi K, Ohnishi Y, Shibata A.  Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.  Blood   1987  70(3):612-9  PubMed ID: 3304457  
1785  Kishi K.  A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.  Leuk Res  1985  9(3):381-90  PubMed ID: 3858609   DOI: 10.1016/0145-2126(85)90060-8
1784  Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.  A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia  Nihon Ketsueki Gakkai Zasshi  1984  47(3):709-18  PubMed ID: 6591673  

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User's Publication
14327  Caillet-Fauquet P, Draps ML, Di Giambattista M, de Launoit Y, Laub R.  Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line  J Virol Methods  2004  121(2):145-53  PubMed ID: 15381351   DOI: 10.1016/j.jviromet.2004.06.010
14328  Caillet-Fauquet P, Di Giambattista M, Draps ML, Sandras F, Branckaert T, de Launoit Y, Laub R.  Continuous-flow UVC irradiation: a new, effective, protein activity-preserving system for inactivating bacteria and viruses, including erythrovirus B19  J Virol Methods  2004  118(2):131-9  PubMed ID: 15081608   DOI: 10.1016/j.jviromet.2004.02.002
15565  Makoto Fujii, Yasushi Tanimoto, Minoru Takata, Kazushi Takao, Noboru Hamada, Toshimitsu Suwaki, Noriko Kawata, Kiyoshi Takahashi, Mine Harada, Mitsune Tanimoto  Association of IgG Fc receptor II with tyrosine kinases in the human basophilic leukemia cell line KU812F  Allergology International  2003  52:149-154    DOI: 10.1046/j.1440-1592.2003.00291.x
2050  Kawata N, Takata M, Suwaki T, Tanimoto Y, Soda R, Takahashi K, Kimura I  Signal transduction by IgG receptors induces calcium mobilization, but not histamine release, in the human basophilic cell line KU812F.  Int Arch Allergy Immunol  1996  109:27-34  PubMed ID: 8527947   DOI: 10.1159/000237228

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