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Cell No. : Cell Name
RCB0497 : KU812F  update : 2021/05/20
CommentChronic myelogeneous leukemia, subclone of RCB0495 KU812.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mizusawa, Hiroshi
Originator Kishi, Kenji
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Classification cancer
History JCRB0104.2
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0616
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 55-64(50) : /59(5),60(7),61(10),62(20),63(2),64(1)
STR(human) OK
Reference information Reference 9
User's Publication 4

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1777  Masuko M, Koike T, Toba K, Kishi K, Kuroha T, Furukawa T, Fuse I, Takahashi M, Kuwano R, Shibata A, Aizawa Y.  Expression of eosinophil peroxidase in the immature basophil cell line KU812-F.  Leuk Res  1999  23(2):99-104  PubMed ID: 10071125
1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127
1775  Valent P, Besemer J, Kishi K, Kaltenbrunner R, Kuhn B, Maurer D, Lechner K, Bettelheim P.  IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites.  J Immunol  1990  145(6):1885-9  PubMed ID: 2144011
1776  Valent P, Besemer J, Kishi K, Di Padova F, Geissler K, Lechner K, Bettelheim P.  Human basophils express interleukin-4 receptors.  Blood  1990  76(9):1734-8 
1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861
1774  Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.  Induction of differentiation of the human leukemia cell line, KU812.  Leuk Res  1987  11(12):1105-13  PubMed ID: 3695564
1765  Fukuda T, Kishi K, Ohnishi Y, Shibata A.  Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.  Blood   1987  70(3):612-9  PubMed ID: 3304457
1785  Kishi K.  A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.  Leuk Res  1985  9(3):381-90  PubMed ID: 3858609
1784  Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.  A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia  Nihon Ketsueki Gakkai Zasshi  1984  47(3):709-18  PubMed ID: 6591673

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User's Publication
15072  Tang Y, Abe N, Yoshimoto M, Zhu B, Murata Y, Nakamura Y.  Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells  Biosci Biotechnol Biochem  2015  79(1):159-63  PubMed ID: 25253661
14327  Caillet-Fauquet P, Draps ML, Di Giambattista M, de Launoit Y, Laub R.  Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line  J Virol Methods  2004  121(2):145-53  PubMed ID: 15381351
14328  Caillet-Fauquet P, Di Giambattista M, Draps ML, Sandras F, Branckaert T, de Launoit Y, Laub R.  Continuous-flow UVC irradiation: a new, effective, protein activity-preserving system for inactivating bacteria and viruses, including erythrovirus B19  J Virol Methods  2004  118(2):131-9  PubMed ID: 15081608
2050  Kawata N, Takata M, Suwaki T, Tanimoto Y, Soda R, Takahashi K, Kimura I  Signal transduction by IgG receptors induces calcium mobilization, but not histamine release, in the human basophilic cell line KU812F.  Int Arch Allergy Immunol  1996  109:27-34  PubMed ID: 8527947

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