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Cell No. : Cell Name
RCB0496 : KU812E  update : 2022/11/04
CommentChronic myelogenous leukemia , subclone of RCB0495 KU812.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mizusawa, Hiroshi
Originator Kishi, Kenji
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Classification cancer
History JCRB0104.1
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0615
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP, MPI
Chromosome mode 57-63(50) : /58(2),59(6),60(7),61(14),62(17),63(3)
STR(human) OK
Reference information Reference 5
User's Publication 0


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Reference
1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127  
1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861  
1774  Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.  Induction of differentiation of the human leukemia cell line, KU812.  Leuk Res  1987  11(12):1105-13  PubMed ID: 3695564   DOI: 10.1016/0145-2126(87)90164-0
1785  Kishi K.  A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.  Leuk Res  1985  9(3):381-90  PubMed ID: 3858609   DOI: 10.1016/0145-2126(85)90060-8
1784  Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.  A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia  Nihon Ketsueki Gakkai Zasshi  1984  47(3):709-18  PubMed ID: 6591673  

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