Cell No. : Cell Name RCB0496 : KU812E  update : 2024/01/17
Comment			Chronic myelogenous leukemia , subclone of RCB0495 KU812.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Mizusawa, Hiroshi
		Originator	Kishi, Kenji
		Year of deposit	1990
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Tissue	blood
		Classification	cancer
		History	JCRB0104.1
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0615
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP, MPI
		Chromosome mode		57-63(50) : /58(2),59(6),60(7),61(14),62(17),63(3)
		STR(human)		OK
Reference information		Reference	7
		User's Publication	1

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Reference

2293  Emi IIMURA, Tadao OHNO  EXAMINATION OF 254 PRIMATE CELL LINES BY A PCR PROTOCOL USEFUL FOR ROUTINE DETECTION OF LATENT HIV-I DNA  TISSUE CULTURE RESEARCH COMMUNICATIONS  1998  17:81-86

1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127

256  Valent P, Besemer J, Kishi K, Kaltenbrunner R, Kuhn B, Maurer D, Lechner K, Bettelheim P.  IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites  J Immunol  1990  145(6):1885-9  PubMed ID: 2144011

1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861

1774  Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.  Induction of differentiation of the human leukemia cell line, KU812.  Leuk Res  1987  11(12):1105-13  PubMed ID: 3695564   DOI: 10.1016/0145-2126(87)90164-0

1785  Kishi K.  A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.  Leuk Res  1985  9(3):381-90  PubMed ID: 3858609   DOI: 10.1016/0145-2126(85)90060-8

1784  Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.  A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia  Nihon Ketsueki Gakkai Zasshi  1984  47(3):709-18  PubMed ID: 6591673

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User's Publication

2316  Emi IIMURA, Tadao OHNO  EXAMINATION OF 254 PRIMATE CELL LINES BY A PCR PROTOCOL USEFUL FOR ROUTINE DETECTION OF LATENT HIV-I DNA  TISSUE CULTURE RESEARCH COMMUNICATIONS  1998  17:81-86    DOI: 10.11418/jtca1981.17.2_81