Cell No. : Cell Name
RCB0496 : KU812E
update : 2024/01/17
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Comment | Chronic myelogenous leukemia , subclone of RCB0495 KU812. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Mizusawa, Hiroshi
|
Originator |
Kishi, Kenji
|
Year of deposit |
1990
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Gender |
Male
|
Tissue |
blood
|
Classification |
cancer
|
History |
JCRB0104.1
|
|
Lifespan |
infinite
|
Morphology |
lymphocyte-like
|
Cellosaurus(Expasy) |
CVCL_0615
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
Culture medium |
|
RPMI1640 + 10% FBS
|
Culture method |
|
|
Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma |
|
(-)
|
Virus (HIV) |
|
(-)
|
Virus (HTLV-1) |
|
(-)
|
Isozyme |
|
LD, NP, MPI
|
Chromosome mode |
|
57-63(50) : /58(2),59(6),60(7),61(14),62(17),63(3)
|
STR(human) |
|
OK
|
Reference information |
Reference |
7
|
User's Publication |
0
|
Reference |
2293
Emi IIMURA, Tadao OHNO
EXAMINATION OF 254 PRIMATE CELL LINES BY A PCR PROTOCOL USEFUL FOR ROUTINE DETECTION OF LATENT HIV-I DNA
TISSUE CULTURE RESEARCH COMMUNICATIONS
1998
17:81-86
|
1796
Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.
Failure to detect IL-3-binding sites on human mast cells.
J Immunol
1990
145:3432-7
PubMed ID: 2230127
|
256
Valent P, Besemer J, Kishi K, Kaltenbrunner R, Kuhn B, Maurer D, Lechner K, Bettelheim P.
IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites
J Immunol
1990
145(6):1885-9
PubMed ID: 2144011
|
1795
Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.
KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.
Blood
1989
73:2003-13
PubMed ID: 2540861
|
1774
Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.
Induction of differentiation of the human leukemia cell line, KU812.
Leuk Res
1987
11(12):1105-13
PubMed ID: 3695564
DOI: 10.1016/0145-2126(87)90164-0
|
1785
Kishi K.
A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Leuk Res
1985
9(3):381-90
PubMed ID: 3858609
DOI: 10.1016/0145-2126(85)90060-8
|
1784
Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.
A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia
Nihon Ketsueki Gakkai Zasshi
1984
47(3):709-18
PubMed ID: 6591673
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